Intestinal permeability is reduced and IL-10 levels are increased in septic IL-6 knockout mice

Abstract

Sepsis is associated with increased intestinal permeability, but mediators and mechanisms are not fully understood. We examined the role of interleukin (IL)-6 and IL-10 in sepsis-induced increase in intestinal permeability. Intestinal permeability was measured in IL-6 knockout (IL-6 -/-) and wild-type (IL-6 +/+) mice 16 h after induction of sepsis by cecal ligation and puncture or sham operation. In other experiments, mice or intestinal segments incubated in Ussing chambers were treated with IL-6 or IL-10. Intestinal permeability was assessed by determining the transmucosal transport of the 4.4-kDa marker fluorescein isothiocyanate conjugated dextran and the 40-kDa horseradish peroxidase. Intestinal permeability for both markers was increased in septic IL-6 +/+ mice but not in septic IL-6 -/- mice. Treatment of nonseptic mice or of intestinal segments in Ussing chambers with IL-6 did not influence intestinal permeability. Plasma IL-10 levels were increased in septic IL-6 -/- mice, and treatment of septic mice with IL-10 resulted in reduced intestinal permeability. Increased intestinal permeability during sepsis may be regulated by an interaction between IL-6 and IL-10. Treatment with IL-10 may prevent the increase in mucosal permeability during sepsis.

Enzyme-Modified Cheese Exerts Inhibitory Effects on Allergen Permeation in Rats Suffering from Indomethacin-Induced Intestinal Inflammation

“…We have reported that NPWDQ (aa 107-111 of s 2 -casein), which occurred in the EMC, inhibited OVA permeation in a Caco-2 cell model. 10) In this study, first we examined to determine whether NPWDQ would inhibit the permeation of FITC-dextran (a marker of paracellular transport 18) ) and HRP (that of transcellular transport 18) ) in vitro. As shown in Fig.…”

Mast cells are required for experimental oral allergen–induced diarrhea

“…In vitro permeability was measured in Ussing chambers as described previously (15). Briefly, two jejunum segments per mouse were opened along the mesenteric border, rinsed, and pinned as intact sheets between siliconized Ussing half chambers.…”

Epithelial Integrity Is Maintained by a Matriptase-Dependent Proteolytic Pathway

“…31 In brief, mice were gavaged with 10 ml/kg of a solution of 22 mg/ml fluorescein isothiocyanate (FITC)-dextran (molecular mass 4 kDa, Sigma) in PBS, pH 7.4. Blood was collected from the inferior vena cava 3 hours after gavage, and plasma was collected by centrifugation at 3000 rpm for 20 minutes at 4°C.…”