Intestinal Permeability and Bacterial Translocation Following Small Bowel Transplantation in the Rat

Grant, David; Hurlbut, David; Zhong, Robert; Wang, Pengzhi; Chen, Huifang; García, Bertha; Behme, R. J.; Stiller, C. R.; Duff, John

doi:10.1097/00007890-199108000-00007

Cited by 83 publications

(26 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Grant et al [40] demon strated increased permeability in rat isografts and in non rejecting allografts early postoperatively and even greater increases in the rejecting allograft [41], Sigalet et al [42] found similar increases in both isografts and allografts. While the full implications of such an increase in perme ability on net absorption of water and electrolytes are not known, the concomitant foreign antigen load entering the mucosa may also induce various immune system me diated effects on unidirectional absorptive and secretory fluxes mediated by the enteric nervous system.…”

Section: Absorption O F Water and Electrolytesmentioning

confidence: 99%

See 1 more Smart Citation

Enteric Physiology of the Transplanted Gut: Absorption and Motility

Sarr¹

1996

Dig Surg

View full text Add to dashboard Cite

Tremendous progress has been made recently in small bowel transplantation. However, our understanding of the enteric function of the transplanted gut remains in its infancy. Unlike many of the other organs commonly transplanted, the function of the transplanted gut appears to be markedly affected by the consequences of the transplantation procedure (denervation, lymphatic disruption, and immune phenomena). This review discusses the effects of gut transplantation on segmental enteric function, concentrating on absorption and motility. Once many of the problems related to the current means of immunosuppression have been better solved, gut transplantation may prove to be an important addition for gastrointestinal surgeons to the care of patients with intestinal insufficiency.

show abstract

Section: Absorption O F Water and Electrolytesmentioning

confidence: 99%

“…Grant et al [40,41] demonstrated a marked increase in uptake of 51Cr-EDTA (and in bacterial translocation) in the rat with onset of rejection.…”

Section: Nutrient Absorptionmentioning

confidence: 99%

Enteric Physiology of the Transplanted Gut: Absorption and Motility

Sarr¹

1996

Dig Surg

View full text Add to dashboard Cite

show abstract

“…Within minutes of ischemia onset, morphologic changes begin at the mucosal level, the layer most sensitive to hypoxic insult [6]. With advancing storage duration (4–6 h), progressive compromise and eventual loss of the mucosa occurs, resulting in decreased barrier function and absorptive capacity (6–9 h) [9]. The intestinal graft simultaneously becomes susceptible to bacterial translocation and increased antigenic stimulus (>9 h) [7,10,11,12,13].…”

Section: Introductionmentioning

confidence: 99%

Different Ischemic Preconditioning Regimens Affecting Preservation Injury of Intestines

et al. 2011

View full text Add to dashboard Cite

Decreasing ischemia-reperfusion injury in intestinal transplantation is of paramount importance for improving graft recovery and function. This study explores the ability of two ischemic preconditioning (IPC) regimens to reduce preservation injury. Sprague-Dawley rats were divided into 3 groups (n = 11 each). In the controls (group C), intestinal grafts were harvested and preserved. IPC was performed either through 4 cycles of mesenteric ischemia of 4 min each followed by 10 min of reperfusion (group BIPC) or 2 ischemic cycles of 12 min each followed by 10 min of reperfusion (group LIPC). Grafts were stored in histidine-tryptophan-ketoglutarate, and samples were taken 0, 3, 6, 9, 12, 18, and 24 h after preservation. Preservation injury was scored using the Park/Chiu scale. Goblet cells (GC), enteroendocrine cells (EEC) and serotonin-producing EEC (SPEEC) were studied for evaluation of the graft conditions. Group C had the most advanced preservation injury followed by group BIPC. GC count was lowest in group C, followed by BIPC. Comparison between groups BIPC and LIPC showed superior parameters (preservation injury, GC, EEC, and SPEEC) in LIPC. In conclusion, an IPC regimen of 2 ischemic cycles of 12 min each followed by 10 min of reperfusion distinctly decreased the preservation injury of intestinal grafts compared with non-manipulated grafts.

show abstract

“…Phase III histology detected in Phase II histology lead to bacterial translocation, explaining the septic complications and graft death. 9 Therefore, the early detection of graft rejection is important to ensure the success of small bowel transplantation, and a number of studies have reported various methods for this. Mucosal biopsy of the graft has been evaluated as the most precise method of detecting graft rejection.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Acetaminophen Absorption Test for Early Detection of Orthotopic Small Bowel Transplant Rejection

2001

View full text Add to dashboard Cite

We evaluated the efficiency of the acetaminophen (AC) absorption test as a marker of graft rejection in orthotopic small bowel transplantation (SBTX) in rats. Brown Norway (BN) rats were used as donors and Lewis (LEW) rats as recipients. Orthotopic allogenic SBTX was accomplished using a cuff technique for vessel anastomosis. Animals were divided into: group A (n = 9), untreated; group B (n = 15), extended small bowel resection; group C (n = 12), syngeneic SBTX without immunosuppressants; group D (n = 15), allogenic SBTX with tacrolimus (0.1 mg/kg per day); group E (n = 15), allogenic SBTX with tacrolimus (0.5mg/kg per day). Serum AC was measured 15 min following the instillation of 0.15 g/kg AC into the stomach on postoperative days (POD) 1, 3, and 7. Grafts were examined histologically. The group D grafts showed progressive acute rejection histologically, from normal on POD 1, to moderate on POD 3, and severe on POD 7. Serum AC in group D decreased significantly from 53.1 +/- 3.9 microg/ml on POD 1, to 35.0 +/- 12.0 microg/ml on POD 3, and 10.9 +/- 5.6 microg/ml on POD 7. No remarkable change was observed in the other groups. Serum AC correlated well with histological changes in rats subjected to SBTX, resulting in acute rejection. The AC absorption test could be useful for detection of progressive graft rejection in clinical SBTX.

show abstract

Intestinal Permeability and Bacterial Translocation Following Small Bowel Transplantation in the Rat

Cited by 83 publications

References 0 publications

Enteric Physiology of the Transplanted Gut: Absorption and Motility

Enteric Physiology of the Transplanted Gut: Absorption and Motility

Different Ischemic Preconditioning Regimens Affecting Preservation Injury of Intestines

Evaluation of the Acetaminophen Absorption Test for Early Detection of Orthotopic Small Bowel Transplant Rejection

Contact Info

Product

Resources

About