Intestinal Osteopontin Protects From Alcohol-induced Liver Injury by Preserving the Gut Microbiome and the Intestinal Barrier Function

Das, Subodh K.; Song, Zhuolun; Han, Hui; Ge, Xiaodong; Désert, Romain; Athavale, Dipti; Komakula, Sai Santosh Babu; Magdaleno, Fernando; Chen, Wei; Lantvit, Daniel D.; Guzman, Grace; Nieto, Natalia

doi:10.1016/j.jcmgh.2022.06.012

Cited by 15 publications

(7 citation statements)

References 58 publications

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“…SPP1, which is also termed osteopontin, is a secretory molecule expressed by a variety of immune cells which has functions including immunomodulation, chemotaxis, and cell adhesion ( Lin et al, 2022 ). SPP1 plays a role in alcohol liver disease ( Apte et al, 2005 ; Seth et al, 2006 ; Lebel et al, 2008 ; Das et al, 2022 ). In the presence of ethanol, SPP1 binds to integrins and CD44 and activates transcription factors AP-1 and NF-κB ( Das et al, 2005 ; Bellahcène et al, 2008 ).…”

Section: Discussionmentioning

confidence: 99%

Ethanol-induced cerebellar transcriptomic changes in a postnatal model of fetal alcohol spectrum disorders: Focus on disease onset

et al. 2023

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Fetal alcohol spectrum disorders (FASD) are a group of neurodevelopmental disorders caused by ethanol exposure in utero, which can result in neurocognitive and behavioral impairments, growth defects, and craniofacial anomalies. FASD affects up to 1-5% of school-aged children in the United States, and there is currently no cure. The underlying mechanisms involved in ethanol teratogenesis remain elusive and need greater understanding to develop and implement effective therapies. Using a third trimester human equivalent postnatal mouse model of FASD, we evaluate the transcriptomic changes induced by ethanol exposure in the cerebellum on P5 and P6, after only 1 or 2 days of ethanol exposure, with the goal of shedding light on the transcriptomic changes induced early during the onset and development of FASD. We have highlighted key pathways and cellular functions altered by ethanol exposure, which include pathways related to immune function and cytokine signaling as well as the cell cycle. Additionally, we found that ethanol exposure resulted in an increase in transcripts associated with a neurodegenerative microglia phenotype, and acute- and pan-injury reactive astrocyte phenotypes. Mixed effects on oligodendrocyte lineage cell associated transcripts and cell cycle associated transcripts were observed. These studies help to elucidate the underlying mechanisms that may be involved with the onset of FASD and provide further insights that may aid in identifying novel targets for interventions and therapeutics.

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Section: Discussionmentioning

confidence: 99%

Ethanol-induced cerebellar transcriptomic changes in a postnatal model of fetal alcohol spectrum disorders: Focus on disease onset

et al. 2023

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“…However, pre-intake of L. plantarum J26 maintained normal mucosal structure and restored the gene expression of Claudin-1, Occludin, and ZO-1 to some extent. Das et al [ 10 ] found that fed mice osteopontin increased gene expression of intestinal tight junction proteins and maintained intestinal barrier function, thereby alleviating ALD. Wang et al [ 38 ] used chitosan to promote the expression of tight junction proteins Claudin-1, Occludin, and ZO-1 to maintain intestinal barrier function, which is consistent with the results of the present study.…”

Section: Discussionmentioning

confidence: 99%

“…In recent years, more and more studies have confirmed the role of probiotics in regulating the gut microbiota and maintaining the intestinal barrier [ 9 , 10 , 11 ]. At the same time, probiotics regulation of gut microbiota to alleviate alcohol-induced liver inflammation is a promising potential therapeutic approach [ 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

Lactobacillus plantarum J26 Alleviating Alcohol-Induced Liver Inflammation by Maintaining the Intestinal Barrier and Regulating MAPK Signaling Pathways

Cheng

Huo

et al. 2022

Nutrients

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Alcoholic liver disease (ALD), as a global health problem, is mainly caused by liver inflammation. Meanwhile, probiotics have been considered as a potential and promising strategy to prevent and alleviate ALD. This study aimed to investigate the ameliorative effect of pre-intaking with Lactobacillus plantarum J26 (L. plantarum J26) on alcohol-induced liver inflammation, with emphasis on the underlying mechanism for alleviating ALD. The results indicated that L. plantarum J26 could reduce the abundance of Gram-negative pathogenic bacteria by regulating the gut microbiota in mice with alcoholic liver injury, thereby reducing the lipopolysaccharide (LPS) content in the intestine. In addition, L. plantarum J26 could also maintain the intestinal barrier, prevent LPS from crossing the intestinal barrier to correct disorders of the gut–liver axis and then inhibit the activation of Toll-like receptor 4 (TLR4)-mediated MAPK signaling pathway, reducing liver inflammation and restoring liver functions. In conclusion, pre-intake of L. plantarum J26 could alleviate alcohol-induced liver inflammation, which may be closely related to the role of intestinal microbiota in regulating and maintaining the intestinal barrier and then regulating the MAPK signaling pathway.

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“…Milk OPN has been shown to bind bacterial lipopolysaccharide in mice treated with an alcoholic diet, thereby lowering tumor necrosis factor-α expression and the subsequent development of alcohol-induced liver damage [94,107]. An investigation of the underlying mechanism showed that milk OPN counteracts the alcohol-induced reduction in the expression of the antimicrobial peptides Reg3b and Reg3g, which regulates bacterial growth in the intestine [108]. It was shown that OPN stimulates the growth of tryptophan-metabolizing and short-chain fatty acid (SCFA)-synthesizing bacteria, including Bifidobacterium, Eubacterium, Prevotella, Allloprevotella, Desulfovibrio, Butyricicoccus, Butyricimonas and Roseburia.…”

Section: Milk Osteopontin and The Gut Microbiomementioning

confidence: 99%

Milk Osteopontin and Human Health

Sørensen

Christensen

2023

Nutrients

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Osteopontin (OPN) is a multifunctional protein found in all vertebrates. OPN is expressed in many different cell types, and is consequently found in most tissues and physiological secretions. OPN is involved in a multitude of biological processes, such as activation and regulation of the immune system; biomineralization; tissue-transformative processes, including growth and development of the gut and brain; interaction with bacteria; and many more. OPN is found in the highest concentrations in milk, where it is believed to initiate and regulate developmental, immunological and physiological processes in infants who consume milk. Processes for the isolation of bovine OPN for use in infant formula have been developed, and in recent years, many studies have investigated the effects of the intake of milk OPN. The purpose of this article is to review and compare existing knowledge about the structure and function of milk OPN, with a particular focus on the effects of milk OPN on human health and disease.

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Intestinal Osteopontin Protects From Alcohol-induced Liver Injury by Preserving the Gut Microbiome and the Intestinal Barrier Function

Cited by 15 publications

References 58 publications

Ethanol-induced cerebellar transcriptomic changes in a postnatal model of fetal alcohol spectrum disorders: Focus on disease onset

Ethanol-induced cerebellar transcriptomic changes in a postnatal model of fetal alcohol spectrum disorders: Focus on disease onset

Lactobacillus plantarum J26 Alleviating Alcohol-Induced Liver Inflammation by Maintaining the Intestinal Barrier and Regulating MAPK Signaling Pathways

Milk Osteopontin and Human Health

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