Intestinal mucosal immune response in ducklings following oral immunisation with an attenuated Duck enteritis virus vaccine

Yang, Xiaoyan; Qi, Xuefeng; Cheng, Anchun; Wang, Mingshu; Zhu, Dekang; Jia, Renyong; Chen, Xiaoyue

doi:10.1016/j.tvjl.2009.04.011

Cited by 26 publications

(20 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hence, the diagnostic assays based on serology are of not much significance, as well as are not useful in acute infection, serological surveillance and while measuring post-vaccination immune response (Toth 1972;Toth & Norcross 1981;Vickery et al 1999a, Vickery et al 1999a, 1999bTang et al 2001;Qi, Yang, Cheng, Wang, Zhu, et al 2009;Tohidi-Esfahani et al 2010;Yang et al 2010). Although cell-mediated immune response has been shown to play a more important role during DEV infection (Vickery et al 1999a(Vickery et al , 1999b, it is possible to detect the anti-DEV neutralizing antibodies (Saade et al 2008;Aravind et al 2012).…”

Section: Immunological / Serological Testsmentioning

confidence: 99%

Duck virus enteritis (duck plague) – a comprehensive update

Dhama

Kumar

Saminathan

et al. 2017

Veterinary Quarterly

View full text Add to dashboard Cite

Duck virus enteritis (DVE), also called duck plague, is one of the major contagious and fatal diseases of ducks, geese and swan. It is caused by duck enteritis virus (DEV)/Anatid herpesvirus-1 of the genus Mardivirus, family Herpesviridae, and subfamily Alpha-herpesvirinae. Of note, DVE has worldwide distribution, wherein migratory waterfowl plays a crucial role in its transmission within and between continents. Furthermore, horizontal and/ or vertical transmission plays a significant role in disease spread through oral-fecal discharges. Either of sexes from varying age groups of ducks is vulnerable to DVE. The disease is characterized by sudden death, vascular damage and subsequent internal hemorrhage, lesions in lymphoid organs, digestive mucosal eruptions, severe diarrhea and degenerative lesions in parenchymatous organs. Huge economic losses are connected with acute nature of the disease, increased morbidity and mortality (5%-100%), condemnations of carcasses, decreased egg production and hatchability. Although clinical manifestations and histopathology can provide preliminary diagnosis, the confirmatory diagnosis involves virus isolation and detection using serological and molecular tests. For prophylaxis, both live-attenuated and killed vaccines are being used in broiler and breeder ducks above 2 weeks of age. Since DEV is capable of becoming latent as well as shed intermittently, recombinant subunit and DNA vaccines either alone or in combination (polyvalent) are being targeted for its benign prevention. This review describes DEV, epidemiology, transmission, the disease (DVE), pathogenesis, and advances in diagnosis, vaccination and antiviral agents/therapies along with appropriate prevention and control strategies.

show abstract

Section: Immunological / Serological Testsmentioning

confidence: 99%

Duck virus enteritis (duck plague) – a comprehensive update

Dhama

Kumar

Saminathan

et al. 2017

Veterinary Quarterly

View full text Add to dashboard Cite

show abstract

“…The virion of DPV is composed of an envelope and a spherical nucleocapsid which contains a double-stranded DNA. DPV can be transmitted among birds by direct contact with infected birds or indirect contact with a contaminated environment (2,(5)(6)(7). Ducks infected with DPV may die without any detectable symptoms or be observed with signs of photophobia, ataxia, and watery diarrhea and a marked reduction in egg production.…”

mentioning

confidence: 99%

An Attenuated Duck Plague Virus (DPV) Vaccine Induces both Systemic and Mucosal Immune Responses To Protect Ducks against Virulent DPV Infection

Huang

Jia

Wang

et al. 2014

Clin Vaccine Immunol

Self Cite

View full text Add to dashboard Cite

c Duck plague (DP) is a severe disease caused by DP virus (DPV). Control of the disease is recognized as one of the biggest challenges in avian medicine. Vaccination is an efficient way to control DPV, and an attenuated vaccine is the main routine vaccine. The attenuated DPV vaccine strain CHa is a modified live vaccine, but the systemic and mucosal immune responses induced by this vaccine have been poorly understood. In this study, the immunogenicity and efficacy of the vaccine were evaluated after subcutaneous immunization of ducks. CD4؉ and CD8 ؉ T cells were counted by flow cytometry, and humoral and mucosal Ig antibodies were analyzed by enzyme-linked immunosorbent assay (ELISA). The results showed that high levels of T cells and Ig antibodies were present postimmunization and that there were more CD4 ؉ T cells than CD8 ؉ T cells. Titers of humoral IgG were higher than those of humoral IgA. Local IgA was found in each sample, whereas local IgG was found only in the spleen, thymus, bursa of Fabricius, harderian gland, liver, bile, and lung. In a protection assay, the attenuated DPV vaccine completely protected ducks against 1,000 50% lethal doses (LD 50 ) of the lethal DPV strain CHv via oral infection. These data suggest that this subcutaneous vaccine elicits sufficient systemic and mucosal immune responses against lethal DPV challenge to be protective in ducks. This study provides broad insights into understanding the immune responses to the attenuated DPV vaccine strain CHa through subcutaneous immunization in ducks.

show abstract

“…Although typically an acute or sometimes chronic and highly contagious disease, DP is characterized by high mortality rates (up to 100%) among domestic (12) and wild ducks, swans, geese, and other waterfowl of different ages. To prevent DP outbreaks on duck farms, attenuated DPV vaccines have been widely used; in China, use of these vaccines is compulsory, with billions of doses administered annually (13,14).…”

Section: Introductionmentioning

confidence: 99%

Isolation and Selection of Duck Primary Cells as Pathogenic and Innate Immunologic Cell Models for Duck Plague Virus

Tian

Cai

et al. 2020

Front. Immunol.

Self Cite

View full text Add to dashboard Cite

Duck plague virus (DPV) is a representative pathogen transmitted among aquatic animals that causes gross lesions and immune inhibition in geese and ducks. The mechanism of organ tropism and innate immune evasion of DPV has not been completely deciphered due to a lack of cell models to study the innate immune manipulation and pathogenicity of aquatic viruses. In the present study, we isolated five types of duck primary cells [duck embryo fibroblasts (DEFs), neurons, astrocytes, peripheral blood mononuclear cells (PBMCs), and monocytes/macrophages] to identify appropriate cell models for DPV, using tropism infection and innate immunologic assays. Cells responded differently to stimulation with DNA viruses or RNA virus analogs. DPV infection exhibited broad tropism, as the recombinant virulent strain (CHv-GFP) infected DEFs, neurons, astrocytes, and monocytes/macrophages, but not the PBMCs, as the expression of EGFP was negligible. The basal levels of innate immunity molecules were highest in monocytes/macrophages and lower in DEFs and astrocytes. Conversely, the titer and genomic copy number of the attenuated virus strain was higher in DEFs and astrocytes than in neurons and monocytes/macrophages. The titer and genomic copy number of the attenuated virus strain were higher compared with the virulent strain in DEFs, neurons, and astrocytes. The innate immune response was not significantly induced by either DPV strain in DEFs, neurons, or astrocytes. The virulent strain persistently infected monocytes/macrophages, but the attenuated strain did so abortively, and this was accompanied by the phenomenon of innate immune inhibition and activation by the virulent and attenuated strains, respectively. Blockage of IFNAR signaling promoted replication of the attenuated strain. Pre-activation of IFNAR signaling inhibited infection by the virulent strain. The selection assay results indicated that induction of innate immunity plays an essential role in controlling DPV infection, and monocytes/macrophages are an Tian et al.Duck Primary Cells Infection Models important cell model for further investigations. Our study provided practical methods for isolating and culturing duck primary cells, and our results will facilitate further investigations of organ tropism, innate immune responses, latent infection, and the effectiveness of antiviral drugs for treating DPV and potentially other aerial bird pathogens.

show abstract

Intestinal mucosal immune response in ducklings following oral immunisation with an attenuated Duck enteritis virus vaccine

Cited by 26 publications

References 24 publications

Duck virus enteritis (duck plague) – a comprehensive update

Duck virus enteritis (duck plague) – a comprehensive update

An Attenuated Duck Plague Virus (DPV) Vaccine Induces both Systemic and Mucosal Immune Responses To Protect Ducks against Virulent DPV Infection

Isolation and Selection of Duck Primary Cells as Pathogenic and Innate Immunologic Cell Models for Duck Plague Virus

Contact Info

Product

Resources

About