Intestinal Microflora in Asiatic Cholera. III. Studies in Pediatric Cholera

Gorbach, Sherwood L.; Mahalanabis, Dilip; Brayton, James B.; Jacobs, Bart C.; Chatterjee, B. D.; Neogy, K. N.

doi:10.1093/infdis/121.1.46

Cited by 15 publications

(23 citation statements)

References 2 publications

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“…Presumably the proportion of pathogenic bacteria in the faecal flora varies with the stage of the disease. Gorbach et al (1970a) found that in the acute phase of cholera, Vibrio cholerae was the predominant organism in the stool and that it decreased in numbers during convalescence.…”

Section: Discussionmentioning

confidence: 98%

Faecal flora of south indian infants and young children in health and with acute gastroenteritis

Albert¹,

Rajan

et al. 1978

Journal of Medical Microbiology

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Section: Discussionmentioning

confidence: 98%

Faecal flora of south indian infants and young children in health and with acute gastroenteritis

Albert¹,

Rajan

et al. 1978

Journal of Medical Microbiology

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“…A few faecal emulsions from AAD patients were not inhibitory, and it is possible that if these patients were exposed to the organism they would have become colonised. Although an antibiotic-mediated alteration of the gut flora is the most likely explanation for this lack of inhibition, it could also be a secondary effect of the induced diarrhoea which can cause changes in the intestinal flora (Levison and Kaye, 1969;Gorbach et al, 1970). When monitoring the growth of C. dzficile in faecal emulsions derived from patients with diarrhoea unrelated to antibiotics, we found that some of them were non-inhibitory; thus these changes could predispose to C. dzficile infection.…”

mentioning

confidence: 84%

An in-vitro model of colonisation resistance to Clostridium difficile infection

Borriello¹,

Barclay²

1986

Journal of Medical Microbiology

122

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Summary.To investigate the importance of the normal gut flora in preventing the establishment of Clostridium dzficile in vivo we have developed an in-vitro test system based on growth in faecal emulsions. Growth of C. dzficile and cytotoxin production are inhibited in faecal emulsions from healthy adults, but not in sterilised emulsions; the importance of viable bacteria in the inhibitory system is evident. Generally, faecal emulsions derived from infants, children and geriatric patients were less inhibitory than those from healthy adults. Those from bottle-fed infants were significantly less inhibitory than those from breast-fed infants. Decreased levels of cytotoxin in the latter group were attributed to the acidic pH of the stools. With the different patient groups studied, faecal samples not inhibitory to C. dzficile in vitro were obtained from 21% of patients with antibiotic-associated diarrhoea, 33% of those taking antibiotics but who did not have diarrhoea, 18.7% of those with diarrhoea unassociated with antibiotics, and 79% of those with C. dzficile-mediated diarrhoea. In some cases inhibition was due to low faecalpH, as in some infants, and in others to other filterable substances. The degree of inhibition could not be linked to specific volatile fatty acids or enzymes.

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“…During convalescence, generally 7 to 10 days after admission, intubation for bacteriology and perfusion was repeated. The details of sampling in the small bowel and collection of fecal specimens have been outlined in previous publications (6,7,10,11).…”

Section: Methodsmentioning

confidence: 99%

“…Quantitative and qualitative studies of intestinal samples were performed in eight media under aerobic and anaerobic conditions (6). To search for enteric pathogens, additional selective media were employed: SS agar, bismuth sulfate agar, MacConkey agar, bile salt agar and TCBS agar (thiosulfate-citrate-bile salt-sucrose, Difco Laboratories, Inc., De-troit, Mich.).…”

Section: Methodsmentioning

confidence: 99%