Intestinal microbiota profiling and predicted metabolic dysregulation in psoriasis patients

Abstract

Objectives The intestinal microbiota has been known to involve in obesity and host immune response. We aimed to investigate the intestinal microbiota and potential genetic function in relation to clinical presentation in psoriasis patients. Methods Faecal microbiota and predicted genetic function inferred from high‐throughput 16S ribosomal RNA sequencing were analysed between psoriasis (n = 32) and age‐, gender‐ and body mass index (BMI)‐matched non‐psoriasis subjects (n = 64), from a referral medical centre. The correlation between altered microbiota and disease activity, arthritis and systemic anti‐psoriatic drugs was also investigated. Results We observed a distinct faecal microbial community structure in psoriasis patients, with an increased abundance of phylum Firmicutes and decreased abundance of phylum Bacteroidetes, across different subgroup of subjects. Ruminococcus and Megasphaera, of the phylum Firmicutes, were the top‐two genera of discriminant abundance in psoriasis. A number of functional genes and metabolic pathways involving bacterial chemotaxis and carbohydrate transport were predicted over‐represented, whereas genes related to cobalamin and iron transport were predicted under‐represented in faecal microbiota of psoriasis patients. Conclusions The distinct faecal microbial composition in psoriasis might be associated with altered transport of carbohydrate, cobalamin and iron, as well as chemotaxis.

The gut microbiome alterations in allergic and inflammatory skin diseases – an update

“…Another study inspecting psoriasis patients faecal microflora composition showed an increased abundance of phylum Firmicutes and decreased abundance of phylum Bacteroidetes . The observations also suggested that the characteristic psoriatic microbial composition might be associated with altered transport of carbohydrate, cobalamin and iron, as well as chemotaxis . In accordance with these reports remains the term ‘psoriatic core intestinal microbiome’, which has been proved to show distinct changes of gut microbiome pattern in comparison with healthy individuals .…”

“…The observations also suggested that the characteristic psoriatic microbial composition might be associated with altered transport of carbohydrate, cobalamin and iron, as well as chemotaxis. 53 In accordance with these reports remains the term 'psoriatic core intestinal microbiome', which has been proved to show distinct changes of gut microbiome pattern in comparison with healthy individuals. 54 As already mentioned, Zanvit et al have demonstrated that mice treated with antibiotics in neonatal life developed exacerbated imiquimod-induced psoriasis in the adulthood and provoked gut and skin microbiota dysfunction in adults, which was associated with increased susceptibility to experimental psoriasis.…”

Claudin‐3 – a new intestinal integrity marker in patients with psoriasis: association with disease severity

“…Patients with psoriasis demonstrate abnormal faecal fat excretion, altered D‐xylose absorption and reduced lactase activity . Simultaneously with intestinal wall inflammation, patients with psoriasis present also an altered gut microbiome termed dysbiosis . Intestinal microbiome continuously interact with enterocytes by direct adhesion and by the release of metabolites .…”

Differences in Gut Microbiota Profiles and Functions Between End-stage Renal Disease and Healthy Populations 

“…Bioinformatics analysis of the 16S rRNA amplicon was conducted as previously described [73]. Brie y, on a per-sample basis, pair-end reads were merged using USEARCH (v8.0.1623), with 8 bp minimum overlap of reading pairs [74].…”