Intestinal microbial ecology and hematological parameters of broiler fed cassava waste pulp fermented with Acremonium charticola

Abstract

Aim:The aim of this study was to evaluate the effect of feeding Acremonium charticola-fermented cassava pulp (AC-FCP) on the intestinal microbial ecology and hematological indices of broiler chickens.Materials and Methods:A total of 240 male Lohman day-old-chicks were randomly allotted to one of the four experimental diets including control diet, control diet + antimicrobials (neomycin; 300 mg/kg diet), diet containing AC-FCP (16 g/100 g diet) or diet containing AC-FCP + antimicrobials. At day 28, the birds from each pen were blood sampled, sacrificed and immediately the internal organs were removed and weighed. Digesta were obtained from the ileum and cecum.Results:Birds fed AC-FCP had lower (p<0.05) coliform bacteria count in the ileal digesta than birds fed control diet or control diet + antimicrobials. Butiric acid was higher (p<0.05) in the cecal content of birds fed AC-FCP than in other birds. Propionic acid was also higher in AC-FCP fed birds than in other birds although statistically not significant. The percentages of lymphocytes and heterophils were higher (p<0.05) and tended (p=0.07) to be lower, respectively, in broilers fed control diet than in other birds. The birds provided control diet had lower (p<0.05) heterophils to lymphocytes ratio compared to those receiving AC-FCP or AC-FCP + antimicrobials. Serum total protein and globulin were higher (p<0.01) in birds fed control diet or control diet + antimicrobials compared to AC-FCP or AC-FCP + antimicrobials fed birds. Serum albumin was lower (p<0.01) in AC-FCP birds than that in other birds. There was a tendency (p=0.09) that birds fed AC-FCP diet had lower total serum cholesterol than other birds.Conclusion:Feeding AC-FCP has potential to improve the intestinal health and protect the birds from acute infections.

Effect of decocted turmeric on performance, hematological parameters and carcass traits of broiler chickens

“…This present finding was in agreement with that of previously reported by Hosseini-Vashan et al (2012) when feeding turmeric rhizome powder to broilers under heat stress conditions. The H/L ratio has commonly been used as an indicator of stress and infections in broilers (Sugiharto et al, 2016a;2016b;2017b). In this basis, decocted turmeric seemed beneficial in alleviating the stress and infections in broiler chickens.…”

“…After centrifugation at 2,000 rpm for 15 min, the serum was obtained and frozen until serum biochemistry analysis. The enumeration of heterophils and lymphocytes, and the analysis of serum biochemistry were determined according to Sugiharto et al (2017b). At day 32, the birds were slaughtered, de-feathered and eviscerated.…”

Effect of feeding duration of <i>Spirulina platensis</i> on growth performance, haematological parameters, intestinal microbial population and carcass traits of broiler chicks

“…The rest of the blood was collected in the vacutainers with no anticoagulant, let to clot at room temperature, and centrifuged at 2000 rpm for 15 minutes to produce serum. The serum was frozen until analyses of antibody titres and serum biochemistry (Sugiharto et al, 2017a). At day 35, a total of 24 chicks were slaughtered, de-feathered and eviscerated.…”

Hematological parameters and selected intestinal microbiota populations in the Indonesian indigenous crossbred chickens fed basal diet supplemented with multi-strain probiotic preparation in combination with vitamins and minerals

“…The selected bacterial populations in the ileal digesta of chicks were determined based on Sugiharto et al . [ 18 ]. Coliform bacteria and lactose-negative Enterobacteriaceae were counted on MacConkey agar (Merck KGaA, Darmstadt, Germany) after aerobic incubation at 38°C for 24 h as red and colorless colonies, respectively.…”