Intestinal methane production is associated with decreased weight loss following bariatric surgery

Mathur, Ruchi; Mundi, Manpreet S.; Chua, Kathleen Shari; Lorentz, Paul A.; Lin, Eugenia; Burch, Miguel; Youdim, Adrienne; Pimentel, Mark

doi:10.1016/j.orcp.2016.06.006

Cited by 17 publications

(18 citation statements)

References 18 publications

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“…Breath methane levels show a good correlation with qPCR of methanogens in stool [ 1 , 2 ] and are established as relevant in the diagnosis of small intestinal bacterial overgrowth (SIBO) [ 3 ]. In addition, increased methane production by intestinal methanogens has also been associated with constipation [ 4 ], constipation-predominant irritable bowel syndrome (IBS-C) [ 5 ], obesity [ 6 ], decreased weight loss after bariatric surgery [ 7 ], multiple sclerosis [ 8 ], and other conditions [ 3 ]. Furthermore, data from both academic and commercial trials are now becoming available, which show an association with the suppression of methane production and improvements in biomarkers or clinical symptoms [ 2 , 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

Selection of a cut-off for high- and low-methane producers using a spot-methane breath test: results from a large north American dataset of hydrogen, methane and carbon dioxide measurements in breath

Gottlieb

Wacher

et al. 2017

Gastroenterol. Rep.

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Background: Levels of breath methane, together with breath hydrogen, are determined by means of repeated collections of both, following ingestion of a carbohydrate substrate, at 15–20 minutes intervals, until 10 samples have been obtained. The frequent sampling is required to capture a rise of hydrogen emissions, which typically occur later in the test: in contrast, methane levels are typically elevated at baseline. If methane emissions represent the principal objective of the test, a spot methane test (i.e. a single-time-point sample taken after an overnight fast without administration of substrate) may be sufficient. Methods: We analysed 10-sample lactulose breath test data from 11 674 consecutive unique subjects who submitted samples to Commonwealth Laboratories (Salem, MA, USA) from sites in all of the states of the USA over a one-year period. The North American Consensus (NAC) guidelines criteria for breath testing served as a reference standard. Results: The overall prevalence of methane-positive subjects (by NAC criteria) was 20.4%, based on corrected methane results, and 18.9% based on raw data. In our USA dataset, the optimal cut-off level to maximize sensitivity and specificity was ≥4 ppm CH4, 94.5% [confidence interval (CI): 93.5–95.4%] and 95.0% (CI: 94.6–95.5%), respectively. The use of a correction factor (CF) (5% CO2 as numerator) led to reclassifications CH4-high to CH4-low in 0.7 % and CH4-low to CH4-high in 2.1%. Conclusions: A cut-off value for methane at baseline of either ≥4 ppm, as in our USA dataset, or ≥ 5 ppm, as described in a single institution study, are both highly accurate in identifying subjects at baseline that would be diagnosed as ‘methane-positive’ in a 10-sample lactulose breath test for small intestinal bacterial overgrowth.

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Section: Introductionmentioning

confidence: 99%

Selection of a cut-off for high- and low-methane producers using a spot-methane breath test: results from a large north American dataset of hydrogen, methane and carbon dioxide measurements in breath

Gottlieb

Wacher

et al. 2017

Gastroenterol. Rep.

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“…An increase or decrease in methane metabolism, as in this study (Fig. S5), always happens in anaerobic bacteria, including Bacteroides spp., which induces its increase or decrease 94 . Moreover, butanoate metabolism plays a critical role in the prevention and treatment of intestinal diseases 95 .…”

Section: Discussionmentioning

confidence: 99%

Effects of enrofloxacin treatment on the bacterial microbiota of milk from goats with persistent mastitis

Polveiro

Vidigal

Mendes

et al. 2020

Sci Rep

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Antibiotic resistance has become a major concern for human and animal health. As fluoroquinolones have been extensively used in human and veterinary medicine, there has also been the rapid emergence and spread of antimicrobial resistance around the world. Here, we analysed the microbiome of goat milk using samples from healthy goats and those diagnosed with persistent mastitis and treated using the antibiotic enrofloxacin with 16S rRNA amplicon sequencing. We selected a group of 11 goats and 22 samples of milk that did not respond clinically to enrofloxacin treatment. Milk samples were evaluated before and after treatment to verify changes of the microbiota; the three first lactating goats were selected from the healthy control group. The milk samples from the healthy control animals presented a larger abundance of different species of bacteria of the Staphylococcus genus, but a smaller number of different genera, which indicated a more specific niche of resident bacteria. The Firmicutes phylum was predominantly different between the studied groups. Samples from before-treatment animals had a higher number of new species than those from the control group, and after being treated again. These microbiota received new bacteria, increasing the differences in bacteria even more in relation to the control group. Genotypes such as Trueperella and Mannheimia, between other genera, had a high abundance in the samples from animals with persistent mastitis. The dysbiosis in this study, with marked evidence of a complex microbiota in activity in cases of the failure of antimicrobial treatment for persistent chronic mastitis, demonstrates a need to improve the accuracy of pathogen identification and increases concern regarding antibiotic treatments in milk production herds. Antimicrobial therapies have been shown to be increasingly problematic due to the development multiple types of antimicrobial resistance (AMR) mechanisms, and for that reason, therapeutic alternatives to treat multidrug-resistant microorganisms are rapidly dwindling. Fluoroquinolones have been extensively used in human and veterinary medicine as they are considered among the most effective drugs for the treatment of bacterial infections 1,2. Enrofloxacin, is a fluoroquinolone exclusively developed for use in veterinary medicine 1,3,4. This drug is a potent inhibitor of bacterial DNA Topoisomerase II (Gyrase) and the DNA Topoisomerase IV (Topo IV), which are essential enzymes involved in key cellular processes including DNA replication 5-10. The drug has a broad spectrum of activity, being active against major pathogenic bacteria (both Gram-positive and Gram-negative), mycoplasmas 11 , and also mycobacteria 12 , but is ineffective against obligate anaerobes 13. Furthermore, in both mammalian and non-mammalian species, enrofloxacin is partially metabolised in the liver to ciprofloxacin, a primary metabolite of which is cyclopropyl, a potent antimicrobial agent itself 14. The active substance is characterised by a low host toxicity, being non-mutagenic with a...

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“…Methane metabolism is always happened in anaerobic bacteria, including the species of Bacteroides spp. and Clostridium spp., and reported to affect the gastrointestinal neuromuscular function of hosts (Mathur et al 2016). The increase of Clostridiales and Bacteroidales probably contribute to the induction of methane metabolism and one carbon pool by folate.…”

Section: Discussionmentioning

confidence: 99%

Coupled changes of bacterial community and function in the gut of mud crab (Scylla Paramamosain) in response to Baimang disease

et al. 2019

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Increasing evidence has revealed a close association between intestinal bacterial community and hosts health. However, it is unclear whether and what extend Baimang disease alters the intestinal microbiota in mud crab ( Scylla paramamosain ). Here, we conducted intestinal contents Illumina sequencing of healthy and Baimang diseased mud crab ( S. paramamosain ) to understand bacterial community variations among health status. In addition, bacterial functional predication was used to investigate whether and how the bacteria variations further change their functions? The phyla of Proteobacteria , Fusobacteria , Cyanobacteria , Tenericutes , Firmicutes , Bacteroidetes , and Spirochaetae constituted over 96.44% of the total intestinal bacteria, with being the dominant taxa. The 7 most significantly different orders, including the increased four orders of Clostridiales , Entomoplasmatales , Bacteroidales , and Mycoplasmatales and the decreased three orders of Vibrionales , Campylobacterales , and Fusobacteriales , accounted for 61.14% dissimilarity, probably being the indicator taxa of Baimang disease. Accordingly, 12 Kyoto Encyclopedia of Genes and Genomes orthologies in level 3 shifted significantly at the diseased crabs. Especially, bacterial secretion system, secretion system, lipopolysaccharide biosynthesis proteins and Vibrio cholerae pathogenic cycle, being related to bacterial virulence, were reduced. In addition, the reduced butanoate metabolism, and induced methane metabolism and one carbon pool by folate were important metabolic processes of probiotic, such as Bacteroides spp. and Clostridium spp., with playing critical roles in host health. This study suggests that Baimang disease coupled altered the intestinal bacterial communities and functions, providing timely information for further analysis the influencing mechanism of Baimang disease in mud crab ( S. paramamosain ). Electronic supplementary material The online version of this article (10.1186/s13568-019-0745-1) contains supplementary material, which is available to authorized users.

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Intestinal methane production is associated with decreased weight loss following bariatric surgery

Cited by 17 publications

References 18 publications

Selection of a cut-off for high- and low-methane producers using a spot-methane breath test: results from a large north American dataset of hydrogen, methane and carbon dioxide measurements in breath

Selection of a cut-off for high- and low-methane producers using a spot-methane breath test: results from a large north American dataset of hydrogen, methane and carbon dioxide measurements in breath

Effects of enrofloxacin treatment on the bacterial microbiota of milk from goats with persistent mastitis

Coupled changes of bacterial community and function in the gut of mud crab (Scylla Paramamosain) in response to Baimang disease

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