Intestinal injury can be reduced by intra-arterial postischemic perfusion with hypertonic saline

Kornyushin, O. V.; Galagudza, M. M.; Kotslova, Anna; Нутфуллина, Г. М.; Shved, Nina; Nevorotin, A. I.; Седов, В. К.; Власов, Т. Д.

doi:10.3748/wjg.v19.i2.209

Cited by 4 publications

(3 citation statements)

References 32 publications

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“…In contrast, gut mucosa of the SMAO-VNS group exhibited only sporadic ischemic changes. In addition, the proportion of the ischemic area was evaluated, as described previously 16 . The difference in the proportion of ischemic areas in the SMAO-VNS group tended to decrease relative to that in the SMAO group (Fig.…”

Section: Resultsmentioning

confidence: 97%

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Vagus nerve stimulation modulates arachidonic acid production in the mesenteric lymph following intestinal ischemia-reperfusion injury

Nakatsutsumi

Yagi

Doki

et al. 2021

J Trauma Acute Care Surg

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BACKGROUND: Inflammatory lipid mediators in mesenteric lymph (ML), including arachidonic acid (AA), are considered to play an important role in the pathogenesis of multiple-organ dysfunction after hemorrhagic shock. A previous study suggested that vagus nerve stimulation (VNS) could relieve shock-induced gut injury and abrogate ML toxicity, resulting in the prevention of multiple-organ dysfunction. However, the detailed mechanism of VNS in lymph toxicity remains unclear. The study aimed to investigate the relationship between VNS and inflammatory lipid mediators in ML. METHODS:Male Sprague-Dawley rats underwent laparotomy and superior mesenteric artery obstruction (SMAO) for 60 minutes to induce intestinal ischemia followed by reperfusion and observation. The ML duct was cannulated, and ML samples were obtained both before and after SMAO. The distal ileum was removed at the end of the observation period. In one group of animals, VNS was performed from 10 minutes before 10 minutes after SMAO (5 V, 0.5 Hz). Liquid chromatography-electrospray ionization-tandem mass spectrometry analysis of AA was performed for each ML sample. The biological activity of ML was examined using a monocyte nuclear factor κ-light-chainenhancer of activated B cells activation assay. Western blotting of phospholipase A 2 group IIA (PLA 2 -IIA) was also performed for ML and ileum samples. RESULTS:Vagus nerve stimulation relieved the SMAO-induced histological gut injury. The concentration of AA and level of nuclear factor κ-lightchain-enhancer of activated B cells activation in ML increased significantly after SMAO, whereas VNS prevented these responses. Western blotting showed PLA 2 -IIA expression in the ML and ileum after SMAO; however, the appearance of PLA2-IIA band was remarkably decreased in the samples from VNS-treated animals. CONCLUSION: The results suggested that VNS could relieve gut injury induced by SMAO and decrease the production of AA in ML by altering PLA 2 -IIA expression in the gut and ML.

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Section: Resultsmentioning

confidence: 97%

“…In addition, the proportion of the ischemic area was evaluated, as described previously. 16 The difference in the proportion of ischemic areas in the SMAO-VNS group tended to decrease relative to that in the SMAO group (Fig. 5, upper panel) (83.9% ± 21.1% in SMAO-VNS group vs. 59.7 ± 42.4% in SMAO group) (Fig.…”

Section: Histological Evaluation Of the Gutmentioning

confidence: 89%

Vagus nerve stimulation modulates arachidonic acid production in the mesenteric lymph following intestinal ischemia-reperfusion injury

Nakatsutsumi

Yagi

Doki

et al. 2021

J Trauma Acute Care Surg

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“…In this way, the findings disorders of mesenteric lymph flow and in vivo intestinal lymphocytes migration and the accompanying increase of endotoxin and TNF-alpha levels in mesenteric lymph, and the lymph drainage could block the "gutlymph" pathway, improve intestinal barrier function, and attenuate distant organ injury in the early stage of gut I/R injury 34,35 . The intestinal post ischemic perfusion with hypertonic saline through the superior mesenteric artery followed by blood flow restoration is a protective procedure that could be used for the prevention of intestinal ischemia and reperfusion injury 36 .…”

Section: Discussionmentioning

confidence: 99%

Hystopathological study of the intestinal epithelium submitted to different times of programmed ischemia and twenty four hours reperfusion

Guillaumon

Couto²

2013

Acta Cir. Bras.

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ABSTRACT:PURPOSE: To investigate the changes in the intestinal epithelium of rats, when submitted to the ischemia of varied time and scheduled reperfusion. METHODS:For this purpose, the following groups were established six groups: control, 10, 20, 30, 40, 60 minutes of ischemia and reperfusion time (24 hours). Their intestines being resected for histopathological analysis: the length and width of the villus, and mitotic index. The nonparametric test Kruskal-Wallis and multiple comparisons test were applied. RESULTS:The qualitatively the intestinal epithelium is regenerated when submitted to different ischemia, and 24 hours reperfusion time. It was observed that morphometry analyzing taking villus length and width and mitotic index as parameter, meaningful alterations were detected. CONCLUSION:The epithelium is regenerated, when subjected to different time planned of ischemia and reperfusion. It was observed:decrease the length and increase the width of the villi, when compared Group VI with I and II, and Group VI with I; increased mitotic index when compared Groups III with I. Every analysis was statistically significant.

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200 mM hypertonic saline resuscitation attenuates intestinal injury and inhibits p38 signaling in rats after severe burn trauma

Sun

Han

Zhi

et al. 2017

Burns

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Intestinal injury can be reduced by intra-arterial postischemic perfusion with hypertonic saline

Abstract: Brief intestinal postischemic perfusion with HTS(365mOsm) through the SMA followed by blood flow restoration is a protective procedure that could be used for the prevention of intestinal IRI.

Cited by 4 publications

References 32 publications

Vagus nerve stimulation modulates arachidonic acid production in the mesenteric lymph following intestinal ischemia-reperfusion injury

Vagus nerve stimulation modulates arachidonic acid production in the mesenteric lymph following intestinal ischemia-reperfusion injury

Hystopathological study of the intestinal epithelium submitted to different times of programmed ischemia and twenty four hours reperfusion

200 mM hypertonic saline resuscitation attenuates intestinal injury and inhibits p38 signaling in rats after severe burn trauma

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