Intestinal injury after thoracic aortic cross-clamping in the pig

Juel, Ingebjørg S.; Solligård, Erik; Lyng, Oddveig; Strømholm, Tonje; Tvedt, Kåre E.; Johnsen, Harald; Jynge, Per; Sæther, Oddbjørn; Aadahl, Petter; Grönbech, Jon Erik

doi:10.1016/j.jss.2003.10.025

Cited by 33 publications

(27 citation statements)

References 41 publications

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“…2 ), which most likely is due to rapid extravasation of fluid from the venous system to the interstitial tissue and peritoneal cavity. Apart from extravasation of blood to the lamina propria, microscopic examination revealed a pattern of intestinal injury in this model that is much alike that found after impeded arterial blood supply to the intestine, without venous obstruction [13,21,25] . As shown previously and confirmed in this study, the surface epithelium of the villi tips was particularly susceptible to hypoxia and ischemia [13,21,25] .…”

Section: Discussionsupporting

confidence: 51%

“…The animals were deprived of food, but not water, for 24 h before the experiment. Anesthesia was induced and maintained as previously described in detail [13,21] . The animals were tracheotomized and mechanically ventilated with 40% oxygen at a rate of 20 breaths/min.…”

Section: Surgical Preparationmentioning

confidence: 99%

“…Tissue blood flow and cardiac output were determined by means of the distribution of color-labeled microspheres according to procedures described in recent studies from our laboratory [21] .…”

Section: Blood Flowmentioning

confidence: 99%

“…In each slide, 10 randomly chosen fields were examined. The epithelial cells within each observation grid square (containing approximately 10 epithelial cells) were classified into four categories as previously described in detail from our laboratory [21] . The first category consisted of the normal columnar epithelium.…”

Section: Morphologymentioning

confidence: 99%

“…Lumen to blood permeability was determined as previously described [21,22] . Briefly, a jejunal loop of 30 cm was ligated at both ends, and 100 ml (10.0 Ci) 14 C-polyethylene glycol (PEG-4000) (Amersham Bioscience, Buckinghamshire, UK) was injected into the lumen.…”

Section: Permeabilitymentioning

confidence: 99%

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Lactate and Glycerol Released to the Intestinal Lumen Reflect Mucosal Injury and Permeability Changes Caused by Strangulation Obstruction

Juel¹,

Solligård

Skogvoll

et al. 2007

Eur Surg Res

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Background: The present study evaluates whether microdialysis of glycerol and lactate reflects mucosal injury and permeability changes after strangulation obstruction of the pig small intestine. Methods: Strangulation obstruction was induced by tightening a rubber band around a small bowel loop until its venous pressure increased to a level just below diastolic aortic pressure (partial strangulation), or further until cessation of flow in the main feeding artery (total strangulation). Mucosal injury and permeability of marker molecules from blood to lumen and vice versa was compared to release of glycerol and lactate to the intestinal lumen. Results: Mucosal injury, hyperpermeability, and release of glycerol were more pronounced after total than after partial strangulation. In animals with partial strangulation there was a complete restitution of the surface epithelium, and luminal glycerol and lumen-to-blood permeability of polyethylene glycol 4000 remained low. Such animals showed a sustained elevation of lactate and blood-to-lumen permeability of fluorescein isothiocyanate dextran after 2 h of partial strangulation, but a decline to baseline levels of these parameters in animals with 1 h partial strangulation. Conclusion: Microdialysis of lactate and glycerol in the intestinal lumen may be used to assess structural and functional changes of the intestinal mucosa after strangulation obstruction.

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Section: Discussionsupporting

confidence: 51%

Section: Surgical Preparationmentioning

confidence: 99%

Section: Blood Flowmentioning

confidence: 99%

Section: Morphologymentioning

confidence: 99%

Section: Permeabilitymentioning

confidence: 99%

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Lactate and Glycerol Released to the Intestinal Lumen Reflect Mucosal Injury and Permeability Changes Caused by Strangulation Obstruction

Juel¹,

Solligård

Skogvoll

et al. 2007

Eur Surg Res

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Effects of a cantaloupe melon extract/wheat gliadin biopolymer during aortic cross-clamping

et al. 2007

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Validation of IC-VIEW fluorescence videography in a rabbit model of mesenteric ischaemia and reperfusion

Toens

Krones

Blum

et al. 2005

Int J Colorectal Dis

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Computer-assisted laser-fluorescence videography is a feasible, reliable, and valid experimental method for the detection of mesenteric blood supply and intestinal microcirculation. Clinical application is conceivable in mesenteric ischaemia and infarction as well as the operative transposition of intestine. As limiting values to identify the irreversible necrosis are not yet defined, further studies have to analyse the clinical impact more precisely.

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Intestinal injury after thoracic aortic cross-clamping in the pig

Cited by 33 publications

References 41 publications

Lactate and Glycerol Released to the Intestinal Lumen Reflect Mucosal Injury and Permeability Changes Caused by Strangulation Obstruction

Lactate and Glycerol Released to the Intestinal Lumen Reflect Mucosal Injury and Permeability Changes Caused by Strangulation Obstruction

Effects of a cantaloupe melon extract/wheat gliadin biopolymer during aortic cross-clamping

Validation of IC-VIEW fluorescence videography in a rabbit model of mesenteric ischaemia and reperfusion

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