Intestinal Infection Due to Enteroaggregative<i>Escherichia coli</i>among Human Immunodeficiency Virus–Infected Persons

Durrer, Petra; Zbinden, Reinhard; Fleisch, Felix; Altwegg, Martin; Ledergerber, Bruno; Karch, Helge; Weber, Rainer

doi:10.1086/315885

Cited by 42 publications

(25 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The discrimination factor used in the initial detection of the EAEC isolates was the PCR assay with primers derived from a probe for AA. Some reports have shown that the correlation of results obtained by the PCR-and probe-based assay and assays for AA may vary by location (11,37,52,55). However, compared to the assay for AA, the PCR-and probe-based detection of EAEC has been found to be simple and specific in many epidemiological studies (12,46).…”

Section: Discussionmentioning

confidence: 99%

Virulence Characteristics and Molecular Epidemiology of Enteroaggregative Escherichia coli Isolates from Hospitalized Diarrheal Patients in Kolkata, India

et al. 2004

View full text Add to dashboard Cite

Enteroaggregative Escherichia coli (EAEC) is an important diarrheal enteropathogen defined by aggregative adherence to cultured epithelial cells. We have detected EAEC from 121 (6.6%) of 1,826 hospitalized patients admitted with diarrhea to the Infectious Diseases Hospital in Kolkata, India. Watery diarrhea was recorded significantly (P ‫؍‬ 0.0142) more often in children. The majority of the EAEC isolates were not serotypeable (62%) and showed resistance to five or more antibiotics (76%). We studied different virulence genes and the molecular epidemiology of 121 EAEC isolates recovered from diarrheal patients. A PCR assay for detection of virulence genes, an assay for determination of clump formation in liquid culture, and a HeLa cell adherence assay were carried out to characterize the EAEC isolates. Investigations were also conducted to correlate the virulence gene profiles with diarrheal symptoms and molecular epidemiology by pulsed-field gel electrophoresis (PFGE). Two or more virulence genes were detected in 109 (90.1%) EAEC isolates. In the cluster analysis, some isolates with specific gene profiles and phenotypes formed a group or subcluster. This study highlights the comparative distributions of three fimbrial adhesins and other virulence genes among EAEC isolates. The diverse virulence gene and PFGE profiles, along with the existence of diverse serotypes and antibiograms, suggests that the EAEC isolates are genetically heterogeneous in Kolkata.

show abstract

Section: Discussionmentioning

confidence: 99%

Virulence Characteristics and Molecular Epidemiology of Enteroaggregative Escherichia coli Isolates from Hospitalized Diarrheal Patients in Kolkata, India

et al. 2004

View full text Add to dashboard Cite

show abstract

“…In a study conducted in Calcutta, India, the same PCR assay performed with similar sensitivity and specificity (7). In a Swiss study investigating the pathogenic role of EAEC among human immunodeficiency virus-infected persons, the pCVD432 PCR assay was demonstrated to better correlate with clinical findings than the cell adherence assay (6). Other studies, however, have provided evidence that pCVD432-probe-negative EAEC strains may also be associated with diarrheal illness (15,20).…”

Section: Discussionmentioning

confidence: 99%

Prevalence of Enteroaggregative Escherichia coli among Children with and without Diarrhea in Switzerland

et al. 2003

Self Cite

View full text Add to dashboard Cite

In a prospective study between July 1999 and September 2000, stool specimens of children below the age of 16 years with (n ‫؍‬ 187) and without (n ‫؍‬ 137) diarrhea were tested for the presence of enterovirulent bacteria by standard culture methods and by PCR. Targets for the PCR were the plasmid pCVD432 for enteroaggregative Escherichia coli (EAEC), the verotoxin 1 and verotoxin 2 genes for enterohemorrhagic E. coli, ipaH for enteroinvasive E. coli (EIEC) and Shigella spp., genes coding for heat-stable and heat-labile toxins for enterotoxigenic E. coli (ETEC), and the eaeA gene for enteropathogenic E. coli. The following bacteria could be associated with diarrhea: Salmonella enterica (P ‫؍‬ 0.001), Campylobacter spp. (P ‫؍‬ 0.036), ETEC (P ‫؍‬ 0.012), and EAEC (P ‫؍‬ 0.006). The detection of EAEC, ETEC, and S. enterica was strongly associated with a history of recent travel outside of Switzerland. EAEC isolates were found in the specimens of 19 (10.2%) of 187 children with diarrhea and in those of 3 (2.2%) of 137 children without diarrhea (P ‫؍‬ 0.006) and were the most frequently detected bacteria associated with diarrhea. Among the children below the age of 5 years, the specimens of 18 (11.9%) of 151 with diarrhea were positive for EAEC, while this agent was found in the specimens of 2 (2.2%) of 91 controls (P ‫؍‬ 0.007). Enteropathogenic E. coli isolates were found in the specimens of 30 (16.4%) of the patients and in those of 15 (10.9%) of the controls, with similar frequencies in all age groups (P > 0.05). We conclude that EAEC bacteria are involved in a significant proportion of diarrhea cases among children. Children younger than 5 years of age are more often affected by EAEC than older children.

show abstract

“…[12][13][14][15] However, there are few studies on the prevalence of diarrheagenic E. coli in pediatric HIV patients. [16][17][18][19][20] The aims of this study were 1) to determine the prevalence of diarrheagenic E. coli in Peruvian children infected with HIV with and without diarrhea, 2) to determine its association with viral load and immunosuppression, and 3) to determine the antimicrobial resistance patterns of diarrheagenic E. coli isolated from these patients.…”

Section: Introductionmentioning

confidence: 99%

Diarrheagenic Escherichia coli in Human Immunodeficiency Virus (HIV) Pediatric Patients in Lima, Perú

Medina¹,

Rivera²,

Romero³

et al. 2010

The American Society of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

Abstract. We conducted a prospective study in three hospitals in Lima in human immunodeficiency virus (HIV) children to determine the frequency of diarrheagenic Escherichia coli. Five E. coli colonies/patients were studied by a multiplex real-time polymerase chain reaction to identify the six currently recognized groups of diarrhea-associated E. coli . We have analyzed 70 HIV-associated diarrheal and 70 control samples from HIV-infected children without diarrhea. Among the diarrheal episodes 19% were persistent, 3% dysenteric, and 33% were associated with moderate or severe dehydration. The diarrheagenic E. coli were the most commonly isolated pathogens in diarrhea (19%) and control samples (26%) ( P = 0.42), including enteroaggregative (6% versus 10%), enteropathogenic (6% versus 10%), and enterotoxigenic E. coli (4% versus 3%), respectively. The HIV-infected children with diarrhea had the worse age-related immunosuppression, higher viral loads, and were on highly active antiretroviral treatment (HAART) less often than HIV-infected children without diarrhea . Diarrheagenic E. coli were highly resistant to ampicillin (74%) and cotrimoxazole (70%).

show abstract

Intestinal Infection Due to EnteroaggregativeEscherichia coliamong Human Immunodeficiency Virus–Infected Persons

Cited by 42 publications

References 16 publications

Virulence Characteristics and Molecular Epidemiology of Enteroaggregative Escherichia coli Isolates from Hospitalized Diarrheal Patients in Kolkata, India

Virulence Characteristics and Molecular Epidemiology of Enteroaggregative Escherichia coli Isolates from Hospitalized Diarrheal Patients in Kolkata, India

Prevalence of Enteroaggregative Escherichia coli among Children with and without Diarrhea in Switzerland

Diarrheagenic Escherichia coli in Human Immunodeficiency Virus (HIV) Pediatric Patients in Lima, Perú

Contact Info

Product

Resources

About