Intestinal hephaestin potentiates iron absorption in weanling, adult, and pregnant mice under physiological conditions

Abstract

Key Points Intestinal Heph may be required to potentiate iron absorption during rapid growth and pregnancy, or when hypoxia stimulates erythropoiesis. Here, the physiological conditions in which Heph is required are defined and other, complementary, intestinal ferroxidases are identified.

A Wearable Iron-Based Implant as an Intramedullary Nail in Tibial Shaft Fracture of Sheep

“…Figure 2 also shows that the cell viability of iron-based implant was lower than the cell viability of stainless steel implant using hemocytometer ( Figure 2(a) ) and MTT assay ( Figure 2(b) ). Iron is a chemical element that is active in the fundamental physiologic processes [ 12 ], but a higher concentration of iron could induce cellular apoptosis [ 19 ]. Therefore, the cell motility rate in iron-based implant was higher.…”

“…Iron has attractive mechanical properties, approaching those of stainless steel. Due to the formation of a complex iron phosphate layer, iron has a low degradation rate [ 11 ] and is required in little quantity [ 12 ]. A lot of iron-based implants research has been conducted, but just a few of them discussed the potential of the iron-based implant in a clinical study chronically.…”

Oral Administration of Ginger-Derived Lipid Nanoparticles and Dmt1 siRNA Potentiates the Effect of Dietary Iron Restriction and Mitigates Pre-Existing Iron Overload in Hamp KO Mice

“…Mice were anesthetized by CO 2 exposure and killed by cervical dislocation. Hemoglobin (Hb) and hematocrit (Hct) levels in whole blood were determined by standard methods [ 18 ]. Plasma non-heme iron levels were measured using a colorimetric method [ 19 ].…”

“…Intestinal iron absorption and body iron distribution in experimental mice were determined after oral administration of 59 Fe, as previously described [ 18 ]. Briefly, mice were provided their final dose of saline, or FA-GDLVs/siRNA, and then three hours later, food was removed from cages (but mice were allowed free access to water during this time).…”

“…The control diet was utilized here as we sought to assess iron absorption when dietary intake was normal (i.e., to increase physiological relevance). The 24-h time point was consistent with our previous studies [ 18 , 20 ], and was chosen as a time when all enteral iron would be absorbed (including the slower phase of iron transport that occurs 12 to ~20 h after dosing) [ 21 ] and before significant non-specific iron losses had occurred (e.g., via desquamation of epidermal cells, exfoliation of intestinal epithelial cells, and in urine and sweat). Whole-carcass, blood, and tissue radioactivity was measured with a WIZARD2 automatic gamma counter (Perkin Elmer).…”