Intestinal fatty acid binding protein (fabp2) in Atlantic salmon (Salmo salar): Localization and alteration of expression during development of diet induced enteritis

Venold, Fredrik F.; Penn, Michael; Thorsen, Jim; Gu, Jinni; Kortner, Trond M.; Krogdahl, Åshild; Bakke, Anne Marie

doi:10.1016/j.cbpa.2012.09.009

Cited by 49 publications

(35 citation statements)

References 60 publications

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“…FABP2 staining was clearly higher at AI than PI. This is in accordance with the significantly higher gene expression levels at AI in T1 and previous observations in fish [30, 50, 51], which is related to the major absorption of nutrients at AI. ALP has been extensively documented to be modulated by dietary intervention in mammalian models [52].…”

Section: Discussionsupporting

confidence: 92%

Dietary Butyrate Helps to Restore the Intestinal Status of a Marine Teleost (Sparus aurata) Fed Extreme Diets Low in Fish Meal and Fish Oil

et al. 2016

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There is a constant need to find feed additives that improve health and nutrition of farmed fish and lessen the intestinal inflammation induced by plant-based ingredients. The objective of this study was to evaluate the effects of adding an organic acid salt to alleviate some of the detrimental effects of extreme plant-ingredient substitution of fish meal (FM) and fish oil (FO) in gilthead sea bream diet. Three experiments were conducted. In a first trial (T1), the best dose (0.4%) of sodium butyrate (BP-70 ®NOREL) was chosen after a short (9-weeks) feeding period. In a second longer trial (T2) (8 months), four diets were used: a control diet containing 25% FM (T2-D1) and three experimental diets containing 5% FM (T2-D2, T2-D3, T2-D4). FO was the only added oil in D1, while a blend of plant oils replaced 58% and 84% of FO in T2-D2, and T2-D3 and T2-D4, respectively. The latter was supplemented with 0.4% BP-70. In a third trial (T3), two groups of fish were fed for 12 and 38 months with D1, D3 and D4 diets of T2. The effects of dietary changes were studied using histochemical, immunohistochemical, molecular and electrophysiological tools. The extreme diet (T2-D3) modified significantly the transcriptomic profile, especially at the anterior intestine, up-regulating the expression of inflammatory markers, in coincidence with a higher presence of granulocytes and lymphocytes in the submucosa, and changing genes involved in antioxidant defences, epithelial permeability and mucus production. Trans-epithelial electrical resistance (Rt) was also decreased (T3-D3). Most of these modifications were returned to control values with the addition of BP-70. None of the experimental diets modified the staining pattern of PCNA, FABP2 or ALPI. These results further confirm the potential of this additive to improve or reverse the detrimental effects of extreme fish diet formulations.

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Section: Discussionsupporting

confidence: 92%

Dietary Butyrate Helps to Restore the Intestinal Status of a Marine Teleost (Sparus aurata) Fed Extreme Diets Low in Fish Meal and Fish Oil

et al. 2016

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“…Although numerous experiments have provided evidence for several biological functions of transcripts were mainly found at AI, in agreement with the previous studies that showed a proximal to distal decrease in the intestine of several fish species [67][68][69], which is related to the major absorption of nutrients at the anterior segment. By contrast, FABP6 transcripts were detected exclusively at PI, the tissue homologous to the mammalian ileum, as well as in the distal region of zebrafish intestine [70], which suggests that FABP6 may have the same role in the uptake of bile salts, which are absorbed in the ileal epithelium of mammals [71].…”

Section: Discussionsupporting

confidence: 91%

Effects of dietary NEXT ENHANCE®150 on growth performance and expression of immune and intestinal integrity related genes in gilthead sea bream (Sparus aurata L.)

Pérez‐Sánchez

Benedito-Palos

Estensoro

et al. 2015

Fish & Shellfish Immunology

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Gilthead sea bream juveniles were fed different doses (0, 50, 100, 200, 300 ppm) of NEXT ENHANCE®150 (NE) for 9 weeks. Feed gain ratio (FGR) was improved by a 10% with all the doses, but feed intake decreased in a dose dependent manner. The optimum inclusion level to achieve maximum growth was set at 100 ppm. The hepatosomatic index did not vary and only at the highest dose, viscerosomatic and splenosomatic indexes were significantly decreased. No significant changes were found in haematological parameters, plasma biochemistry, total antioxidant capacity and respiratory burst. In a second trial, NE was given at 100 ppm alone (D1) or in combination with the prebiotic PREVIDA® (0.5%) (PRE) (D2) for 17 weeks. There were no differences in the growth rates, and FGR was equally improved for D1 and D2. No significant changes in haematology and plasma antioxidant capacity were detected. The histological examination of the liver and the intestine showed no outstanding differences in the liver, but the number of mucosal foldings appeared to be higher in D1 and D2 vs CTRL diet and the density of enterocytes and goblet cells also appeared higher, particularly in the anterior intestine. A 87-gene PCR-array was constructed based on our transcriptomic database (www.nutrigroup-iats.org/seabreamdb) and applied to samples of anterior (AI) and posterior (PI) intestine. It included 54 new gene sequences and other sequences as markers of cell differentiation and proliferation, intestinal architecture and permeability, enterocyte mass and epithelial damage, interleukins and cytokines, pattern recognition receptors (PRR), and mitochondrial function and biogenesis. More than half of the studied genes had significantly different expression between AI and PI segments. The functional significance of this differential tissue expression is discussed. The experimental diets induced significant changes in the expression of 26 genes. The intensity of these changes and the number of genes that were significantly regulated were higher at PI than at AI. At PI, both diets invoked a clear down-regulation of genes involved in cell differentiation and proliferation, some involved in cell to cell communication, cytokines and several PRR. By contrast, up-regulation was mostly found for genes related to enterocyte mass, cell epithelial damage and mitochondrial activity at AI. The changes were of the same order for D1 and D2, except for fatty acid-binding proteins 2 and 6 and the PRR fucolectin, which were higher in D2 and D1 fed fish, respectively. Thus, NE alone or in combination with PRE seems to induce an anti-inflammatory and anti-proliferative transcriptomic profile with probable improvement in the absorptive capacity of the intestine that would explain the improved FGR.

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“…; Venold et al . ), and its nutritional control has also been demonstrated (Jordal et al . ; Torstensen et al .…”

Section: Discussionmentioning

confidence: 92%

Effects of fish oil replacement by a vegetable oil blend on digestibility, postprandial serum metabolite profile, lipid and glucose metabolism of European sea bass (Dicentrarchus labrax) juveniles

et al. 2014

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Metabolism Aquaculture, Saint-P ee-sur-Nivelle, France A digestibility trial was conducted to evaluate the effects of dietary fish oil (FO) replacement by a vegetable oil blend (VO) (rapeseed, linseed and palm oils) on nutrient digestibility, lipid absorption and transport and lipid and carbohydrate metabolic pathways of Dicentrarchus labrax juveniles. Following 36 days, the liver and intestine of fish were sampled 6 h after the last meal. Remaining fish were fed one more week and blood was collected at different postprandial time intervals. Dietary VO decreased apparent digestibility coefficients (ADC) of lipid and energy. Among the serum parameters analysed, only high-density lipoprotein was affected, being higher in VO group. Hepatic and intestinal expression of genes related to lipid transport and lipoprotein metabolism (FABP, DGAT, ApoB, ApoA4, ApoA1, MTP) showed no differences in transcript levels. No alteration in genes related to cholesterol biosynthesis (HMGCR), glucose transport (GLUT2) or metabolism (GK, PK, G6Pase and PEPCK) in the liver and intestine was found. However, hepatic transcript levels of FADS2 and elovl5 were up-regulated in VO group. In conclusion, replacement of FO by VO did not induce major alterations at molecular and biochemical levels, although a nutritional modulation of LC-polyunsaturated fatty acids biosynthesis pathway was observed.

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Intestinal fatty acid binding protein (fabp2) in Atlantic salmon (Salmo salar): Localization and alteration of expression during development of diet induced enteritis

Cited by 49 publications

References 60 publications

Dietary Butyrate Helps to Restore the Intestinal Status of a Marine Teleost (Sparus aurata) Fed Extreme Diets Low in Fish Meal and Fish Oil

Dietary Butyrate Helps to Restore the Intestinal Status of a Marine Teleost (Sparus aurata) Fed Extreme Diets Low in Fish Meal and Fish Oil

Effects of dietary NEXT ENHANCE®150 on growth performance and expression of immune and intestinal integrity related genes in gilthead sea bream (Sparus aurata L.)

Effects of fish oil replacement by a vegetable oil blend on digestibility, postprandial serum metabolite profile, lipid and glucose metabolism of European sea bass (Dicentrarchus labrax) juveniles

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