Intestinal edema decreases intestinal contractile activity via decreased myosin light chain phosphorylation

Uray, Karen; Laine, Glen A.; Xue, Hasan; Allen, Steven J.; Cox, Charles S.

doi:10.1097/01.ccm.0000239195.06781.8c

Cited by 37 publications

(65 citation statements)

References 24 publications

(29 reference statements)

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“…These data suggest that MLC phosphatase activity is increased. These data agree with our findings in the in vivo intestinal edema model in which intestinal smooth muscle MLC and MYPT1 phosphorylation is decreased (7,14). Previous studies have indicated that PAK is responsive to mechanical signals and can regulate MLC phosphatase activity (22,27,39,40).…”

Section: Effect Of Mechanical Stretching On Mlc Phosphorylation-supporting

confidence: 83%

“…Intestinal edema was induced in male Sprague-Dawley rats weighing between 250 and 350 g as described previously (14). Briefly, intestinal edema was induced by a combination of mesenteric venous hypertension and fluid administration (80 ml/kg, 0.9% NaCl).…”

Section: Methodsmentioning

confidence: 99%

“…Intestinal Tissue Contractile Activity-Intestinal contractile activity, in the longitudinal axis, was measured 6 h after surgery as described previously (14). Isometric force was monitored by an external force displacement transducer (Experimetria Ltd., Budapest, Hungary) connected to a PowerLab (AD Instruments, Colorado Springs, CO).…”

Section: Methodsmentioning

confidence: 99%

“…Intestinal edema causes a 6-fold increase in strain in the smooth muscle layer of the intestine compared with non-edematous tissue (11). This increased strain, resulting in increased stretching force to the intestinal smooth muscle cells, is associated with decreased intestinal contractile activity via decreased myosin light chain (MLC) 2 phosphorylation (12)(13)(14)(15). This is accomplished by edema-induced attenuation of the constitutive inhibition of MLC phosphatase in intestinal smooth muscle (7).…”

mentioning

confidence: 99%

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Biphasic Regulation of Myosin Light Chain Phosphorylation by p21-activated Kinase Modulates Intestinal Smooth Muscle Contractility

Chu

Pham

Olate

et al. 2013

Journal of Biological Chemistry

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Background: Myosin light chain (MLC) phosphorylation is the driving force of cell contractility. Results: p21-activated kinase (PAK) can either activate or inhibit myosin light chain phosphorylation, depending on the level of PAK activation. Conclusion: Inhibition of intestinal motility by supraphysiological mechanical stretch is attributable to increased PAK activity. Significance: The biphasic regulation of PAK may provide an explanation for the conflicting observations on PAK-mediated MLC phosphorylation.

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Section: Effect Of Mechanical Stretching On Mlc Phosphorylation-supporting

confidence: 83%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Biphasic Regulation of Myosin Light Chain Phosphorylation by p21-activated Kinase Modulates Intestinal Smooth Muscle Contractility

Chu

Pham

Olate

et al. 2013

Journal of Biological Chemistry

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“…In an interesting study, Uray et al [113] found that the combination of fluid resuscitation and venous hypertension (mimicking IAH) resulted in the highest gut wet-to-dry ratio and gut oedema decreased myosin light chain phosphorylation leading to decreased intestinal contractility. The decrease in contractility may explain why IAH is associated with frequent failure to establish successful enteral nutrition in critically ill patients.…”

Section: Intestinal Oedema Formation In Critically Ill Patients and Imentioning

confidence: 99%

Abdominal pressure and gastrointestinal function: an inseparable couple?

Blaser¹,

Malbrain²,

Regli³

2017

Anaesthesiol Intensive Ther

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Evaluating the degree of organ dysfunction is a cornerstone in distinguishing patients with critical illness from those without. However, evaluation of the gastrointestinal function in critically ill patients is not unified, and is still largely based on subjective clinical evaluation. Although intra-abdominal pressure has been proposed as a parameter to facilitate monitoring of abdominal compartment in critical illness, the interactions between intra-abdominal pressure and gastrointestinal function are poorly clarified. The aim of this current review is to describe interactions and associations between gastrointestinal dysfunction and intra-abdominal pressure from a pathophysiological and clinical point of view.

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Pulse pressure variation guided goal-direct fluid therapy decreases postoperative complications in elderly patients undergoing laparoscopic radical resection of colorectal cancer: a randomized controlled trial

Wu,

Zhao,

Fan

et al. 2024

Int J Colorectal Dis

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Objective The use of goal-directed fluid therapy (GDFT) has been shown to reduce complications and improve prognosis in high-risk abdominal surgery patients. However, the utilization of pulse pressure variation (PPV) guided GDFT in laparoscopic surgery remains a subject of debate. We hypothesized that utilizing PPV guidance for GDFT would optimize short-term prognosis in elderly patients undergoing laparoscopic radical resection for colorectal cancer compared to conventional fluid therapy. Methods Elderly patients undergoing laparoscopic radical resection of colorectal cancer were randomized to receive either PPV guided GDFT or conventional fluid therapy and explore whether PPV guided GDFT can optimize the short-term prognosis of elderly patients undergoing laparoscopic radical resection of colorectal cancer compared with conventional fluid therapy. Results The incidence of complications was significantly lower in the PPV group compared to the control group (32.8% vs. 57.1%, P = .009). Additionally, the PPV group had a lower occurrence of gastrointestinal dysfunction (19.0% vs. 39.3%, P = .017) and postoperative pneumonia (8.6% vs. 23.2%, P = .033) than the control group. Conclusion Utilizing PPV as a monitoring index for GDFT can improve short-term prognosis in elderly patients undergoing laparoscopic radical resection of colorectal cancer. Registration number ChiCTR2300067361; date of registration: January 5, 2023.

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Intestinal edema decreases intestinal contractile activity via decreased myosin light chain phosphorylation

Abstract: We conclude that edema decreases myosin light chain phosphorylation, leading to decreased intestinal contractile activity.

Cited by 37 publications

References 24 publications

Biphasic Regulation of Myosin Light Chain Phosphorylation by p21-activated Kinase Modulates Intestinal Smooth Muscle Contractility

Biphasic Regulation of Myosin Light Chain Phosphorylation by p21-activated Kinase Modulates Intestinal Smooth Muscle Contractility

Abdominal pressure and gastrointestinal function: an inseparable couple?

Pulse pressure variation guided goal-direct fluid therapy decreases postoperative complications in elderly patients undergoing laparoscopic radical resection of colorectal cancer: a randomized controlled trial

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