Intestinal Dysbiosis in the Infant and the Future of Lacto-Engineering to Shape the Developing Intestinal Microbiome

Zeinali, Lida; Giuliano, Shayne; Lakshminrusimha, Satyan; Underwood, Mark A.

doi:10.1016/j.clinthera.2021.11.005

Cited by 2 publications

(1 citation statement)

References 215 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The presence, induction, and activities of enzymes related to the catabolism of nutrients and xenobiotics and their changes due to illness have been determined not only in the serum, liver, and intestine from human but also in gastrointestinal microbiota. Acetate kinase, sucrase, lactase, and lipase have been extensively studied (Kushkevych, 2014;Lin et al, 2022;Zeinali et al, 2022). On the other hand, enzymes related to protection against oxidative stress have also been determined in microbiota studies (Yu et al, 2021;Panigrahi et al, 2022;Zhu et al, 2022).…”

Section: Metabolic Properties Of Cultured Gastrointestinal Microbiotamentioning

confidence: 99%

Cultivation of gastrointestinal microbiota in a new growth system revealed dysbiosis and metabolic disruptions in carcinoma-bearing rats

et al. 2022

View full text Add to dashboard Cite

A challenge in the study of gastrointestinal microbiota (GITm) is the validation of the genomic data with metabolic studies of the microbial communities to understand how the microbial networks work during health and sickness. To gain insights into the metabolism of the GITm, feces from healthy and sick rats with cancer were inoculated in a defined synthetic medium directed for anaerobic prokaryote growth (INC-07 medium). Significant differences between cultures of healthy and sick individuals were found: 1) the consumption of the carbon source and the enzyme activity involved in their catabolism (e.g., sucrase, lactase, lipases, aminotransferases, and dehydrogenases); 2) higher excretion of acetic, propionic, isobutyric, butyric, valeric, and isovaleric acids; 3) methane production; 4) ability to form biofilms; and 5) up to 500 amplicon sequencing variants (ASVs) identified showed different diversity and abundance. Moreover, the bowel inflammation induced by cancer triggered oxidative stress, which correlated with deficient antioxidant machinery (e.g., NADPH-producing enzymes) determined in the GITm cultures from sick individuals in comparison with those from control individuals. Altogether, the data suggested that to preserve the microbial network between bacteria and methanogenic archaea, a complete oxidation of the carbon source may be essential for healthy microbiota. The correlation of 16S rRNA gene metabarcoding between cultures and feces, as well as metabolomic data found in cultures, suggest that INC-07 medium may be a useful tool to understand the metabolism of microbiota under gut conditions.

show abstract

Section: Metabolic Properties Of Cultured Gastrointestinal Microbiotamentioning

confidence: 99%

Cultivation of gastrointestinal microbiota in a new growth system revealed dysbiosis and metabolic disruptions in carcinoma-bearing rats

et al. 2022

View full text Add to dashboard Cite

show abstract

Breast Milk and Breastfeeding: Benefits, Barriers, Maternal Predictors, and Opportunities for Innovation

Sen

2022

Clinical Therapeutics

View full text Add to dashboard Cite

Intestinal Dysbiosis in the Infant and the Future of Lacto-Engineering to Shape the Developing Intestinal Microbiome

Cited by 2 publications

References 215 publications

Cultivation of gastrointestinal microbiota in a new growth system revealed dysbiosis and metabolic disruptions in carcinoma-bearing rats

Cultivation of gastrointestinal microbiota in a new growth system revealed dysbiosis and metabolic disruptions in carcinoma-bearing rats

Breast Milk and Breastfeeding: Benefits, Barriers, Maternal Predictors, and Opportunities for Innovation

Contact Info

Product

Resources

About