Intestinal colonization potential of turbot (Scophthalmus maximus)- and dab (Limanda limanda)-associated bacteria with inhibitory effects against Vibrio anguillarum

Olsson, J.; Westerdahl, A; Conway, Patricia L.; Kjelleberg, Staffan

doi:10.1128/aem.58.2.551-556.1992

Cited by 166 publications

(77 citation statements)

References 25 publications

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“…In conclusion, probiotics in vivo may become more competitive than predicted from experimental observations of their growth patterns or their starting population density as the production of antimicrobial metabolites or better attachment ability lead to inhibition in vitro. The production or e¡ect of inhibitory metabolites depends on the media on which the probiotic is cultured [39]. Further tests examining the production of antimicrobial metabolites in ¢sh mucus need to be carried out to verify preliminary conclusions from this study and account for the factors that a¡ect the ranking of candidate probiotics in vivo.…”

Section: Discussionmentioning

confidence: 92%

In vitro growth characteristics of five candidate aquaculture probiotics and two fish pathogens grown in fish intestinal mucus

Vine

Leukes

Kaiser

2004

FEMS Microbiology Letters

142

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The selection of probiotics for aquaculture is usually based on their antagonism towards pathogens. However, other criteria such as growth, attachment to intestinal mucus and production of beneficial compounds should also be considered. We suggest a protocol for the isolation and selection of potential probiotic bacteria based on their in vitro growth characteristics and propose a ranking index (RI) to screen potential aquaculture probionts. We suggest that the lag period and doubling time are the most important criteria for the comparison of growth curves, hence the RI is based on the doubling time (t d ) and lag period (V) obtained from the growth profile of each bacterium. Bacteria were isolated from the gut of the common clownfish, Amphiprion percula, and screened for antagonistic activity towards seven aquatic pathogens. All five candidate probiotics showed antagonism to various aquatic pathogens. When grown in intestinal fish mucus no probiotic had a RI higher than the two tested pathogens (Aeromonas hydrophila and Vibrio alginolyticus). However, candidate probiont AP1 had a faster specific growth rate (W) (0.05) than the pathogens (0.049 and 0.047 respectively), while AP5 grown in marine broth had a shorter lag period than the pathogens. Strategies to increase probiotic concentration include the inoculation of high concentrations and the preconditioning of these bacteria to reduce the lag period. It should be tested whether or not such strategies will allow the probiotic bacteria to dominate initially and thereby gain a competitive advantage. This could become an important aspect under in vivo conditions where both attachment and nutrient supply differ from that found in in vitro studies.

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Section: Discussionmentioning

confidence: 92%

In vitro growth characteristics of five candidate aquaculture probiotics and two fish pathogens grown in fish intestinal mucus

Vine

Leukes

Kaiser

2004

FEMS Microbiology Letters

142

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“…Only Mueller-Hinton agar supplemented with 2% NaCl allowed the demonstration of inhibitory effects of the tested bacteria. Olsson et al (1992) previously found differences in the bacteriocin production of intestinal fish microbiota against V. anguillarum depending on the media used.…”

Section: Inhibitory Effectsmentioning

confidence: 97%

Vibrios isolated from the cultured manila clam (Ruditapes philippinarum): numerical taxonomy and antibacterial activities

et al. 2002

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Aims: A numerical taxonomic study of halophilic Vibrio isolated from healthy and brown ring disease (BRD) affected manila clams (Ruditapes philippinarum), harvested from the Atlantic coast of south-western Spain, was performed. Methods and Results: Characterization of 123 presumptive Vibrio spp. was carried out using 94 phenotypic tests. Simple matching and Jaccard similarity coefficients were used for numerical analysis. Cluster analysis by the unweighted pair group method with arithmetic averages yielded 15 phena defined at 0AE81 similarity. Large phena corresponded to Vibrio tubiashii, V. splendidus biotype I and V. harveyi (phena 1, 5 and 9, respectively). The species V. splendidus biotype II, V. natriegens, V. mediterranei and V. alginolyticus were also represented. The inhibitory effect of diffusible extracellular products of the isolates against 27 strains of V. tapetis, the aetiological agent of BRD, was also investigated. Only five V. tubiashii isolates inhibited the growth of V. tapetis strains. The antimicrobial effect was inhibited by heating and depended on the culture medium. Conclusions: The main Vibrio species associated with manila clams were V. tubiashii, V. spendidus and V. harveyi. The antagonistic relationship established between V. tapetis and the Vibrio spp. clam microbiota may explain the failure of isolation in plating medium of V. tapetis from BRD-affected clams on the south Atlantic coast of Spain. Significance and Impact of the Study: Some of the strains isolated from manila clams correspond to agarolytic strains that constitute phenon 7 and they do not fit into any of the currently described Vibrio species.

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“…The production of antibacterial substances by bacteria isolated from the digestive tract of fish has been demonstrated in several studies (Schrùder, Clausen, Sandberg & Raa 1980;Strùm 1988;Westerdahl, Olsson, Kjelleberg & Conway 1991;Olsson, Westerdahl, Conway & Kjelleberg 1992;Bergh 1995;Sugita, Shibuya, Shimooka & Deguchi 1996;Jo Èborn, Olsson, Westerdahl, Conway & Kjelleberg 1997;Sugita, Shibuya, Hanada & Deguchi 1997;Gram, Melchiorsen, Spanggard, Huber & Nielsen 1999;Ringù 2000;Ringù, Bendiksen, Wesmajervi, Olsen, Jansen & Mikkelsen 2000). Therefore, the final aim was to evaluate the antibacterial potential of autochthonous bacteria isolated from the hindgut of Arctic charr before and after challenge with A. salmonicida.…”

Section: Introductionmentioning

confidence: 99%

The effects of soybean, linseed and marine oils on aerobic gut microbiota of Arctic charrSalvelinus alpinusL. before and after challenge withAeromonas salmonicidassp.salmonicida

Ringø

Lødemel

Myklebust

et al. 2002

Aquaculture Research

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Populations of heterotrophic bacteria present in the hindgut region of Arctic charr Salvelinus alpinus L. fed dietary soybean, linseed and marine oils before challenge with Aeromonas salmonicida ssp. salmonicida and marine oil after challenge were estimated using the dilution plate technique. There were differences in bacterial composition between the rearing groups before and after challenge, as well as interindividual variations. For example, carnobacteria were only isolated from the hindgut region of fish fed soybean oil and linseed oil before challenge, whereas Carnobacterium spp. and Carnobacterium funditum‐like species were isolated from fish fed the same oils after challenge. Three non‐motile Aeromonas spp. were isolated from infected fish fed marine oil. One of these isolates was identified as identical to A. salmonicida ssp. salmonicida used in&the challenge test by microbial fingerprinting (amplified fragment length polymorphism). Electron microscopic examinations of hindgut regions demonstrated substantial numbers of bacterial cells associated with enterocytes, but bacterial colonization of the enterocyte surface varied between different rearing groups. The potential of bacteria found associated with the hindgut region to inhibit the fish pathogens A. salmonicida, Vibrio salmonicida and Vibrio anguillarum differed between rearing groups.

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Intestinal colonization potential of turbot (Scophthalmus maximus)- and dab (Limanda limanda)-associated bacteria with inhibitory effects against Vibrio anguillarum

Cited by 166 publications

References 25 publications

In vitro growth characteristics of five candidate aquaculture probiotics and two fish pathogens grown in fish intestinal mucus

In vitro growth characteristics of five candidate aquaculture probiotics and two fish pathogens grown in fish intestinal mucus

Vibrios isolated from the cultured manila clam (Ruditapes philippinarum): numerical taxonomy and antibacterial activities

The effects of soybean, linseed and marine oils on aerobic gut microbiota of Arctic charrSalvelinus alpinusL. before and after challenge withAeromonas salmonicidassp.salmonicida

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