Intestinal cancer development in response to oral infection with high-fat diet-induced Type 2 diabetes (T2D) in collaborative cross mice under different host genetic background effects

Lone, Iqbal M.; Midlej, Kareem; Nun, Nadav Ben; Iraqi, Fuad A.

doi:10.1007/s00335-023-09979-y

Cited by 16 publications

(25 citation statements)

References 47 publications

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“…The Collaborative Cross (CC) mice model was developed as a consequence of the need to create a high genetic diversity mouse reference population [ 86 , 87 ]. The resource comprises a diverse range of recombinant inbred (RI) lines that were produced from a genetically heterogeneous collection of eight progenitor strains.…”

Section: Discussionmentioning

confidence: 99%

The Complexity of Skeletal Transverse Dimension: From Diagnosis, Management, and Treatment Strategies to the Application of Collaborative Cross (CC) Mouse Model

Watted,

Lone,

Midlej

et al. 2024

JFMK

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This study investigates the significance of skeletal transverse dimension (STD) in orthodontic therapy and its impact on occlusal relationships. The primary goal is to enhance understanding and promote the integration of transverse skeletal diagnostics into routine orthodontic assessments. To achieve this aim, the study employs a comprehensive approach, utilizing model analysis, clinical assessments, radiographic measurements, and occlusograms. The initial step involves a meticulous assessment of deficiencies in the maxilla, mainly focusing on transverse dimension issues. Various successful diagnostic methods are employed to ascertain the type and presence of these deficiencies. Furthermore, the study compares surgically assisted maxillary expansion (SARME) and orthopedic maxillary expansion (OME) in addressing skeletal transverse issues. Stability assessments and efficacy analyses are conducted to provide valuable insights into the superiority of SARME over OME. The findings reveal that proper evaluation of STD is crucial in orthodontic diagnosis, as overlooking transverse dimension issues can lead to complications such as increased masticatory muscle activity, occlusal interferences, and an elevated risk of gingival recession. Surgically assisted maxillary expansion emerges as a more stable solution than orthopedic methods. In conclusion, incorporating skeletal transverse diagnostics into routine orthodontic assessments is imperative for achieving optimal occlusal relationships and minimizing negative consequences on dentition, periodontium, and joints. The study emphasizes the significance of accurate three-dimensional assessments and recommends the consideration of SARME over OME for addressing skeletal transverse deficiencies. Finally, the Collaborative Cross (CC) mouse model is also a novel mouse model for studying complex traits. Exploring the Collaborative Cross mouse model opens avenues for future research, promising further insights into transverse skeletal issues in orthodontics.

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Section: Discussionmentioning

confidence: 99%

The Complexity of Skeletal Transverse Dimension: From Diagnosis, Management, and Treatment Strategies to the Application of Collaborative Cross (CC) Mouse Model

Watted,

Lone,

Midlej

et al. 2024

JFMK

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“…This phase aims to collect recombinant events, reorganize founder genomes, and represent all eight founder haplotypes in newly created CC lines. The next stage is inbreeding, brother–sister mating, which continues for nearly 20 generations to achieve around 99% homozygosity with nearly equal contributions from the eight founder strains 93,94 . Litters from 20 and more inbreeding generations will be syngeneic (have the same genetic composition), which will benefit future research.…”

Section: Modeling Population Diversity—the CC Mouse Model For Cancer ...mentioning

confidence: 99%

Towards system genetics analysis of head and neck squamous cell carcinoma using the mouse model, cellular platform, and clinical human data

Zohud,

Lone,

Nashef

et al. 2023

Anim Models and Exp Med

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Head and neck squamous cell cancer (HNSCC) is a leading global malignancy. Every year, More than 830 000 people are diagnosed with HNSCC globally, with more than 430 000 fatalities. HNSCC is a deadly diverse malignancy with many tumor locations and biological characteristics. It originates from the squamous epithelium of the oral cavity, oropharynx, nasopharynx, larynx, and hypopharynx. The most frequently impacted regions are the tongue and larynx. Previous investigations have demonstrated the critical role of host genetic susceptibility in the progression of HNSCC. Despite the advances in our knowledge, the improved survival rate of HNSCC patients over the last 40 years has been limited. Failure to identify the molecular origins of development of HNSCC and the genetic basis of the disease and its biological heterogeneity impedes the development of new therapeutic methods. These results indicate a need to identify more genetic factors underlying this complex disease, which can be better used in early detection and prevention strategies. The lack of reliable animal models to investigate the underlying molecular processes is one of the most significant barriers to understanding HNSCC tumors. In this report, we explore and discuss potential research prospects utilizing the Collaborative Cross mouse model and crossing it to mice carrying single or double knockout genes (e.g. Smad4 and P53 genes) to identify genetic factors affecting the development of this complex disease using genome‐wide association studies, epigenetics, microRNA, long noncoding RNA, lncRNA, histone modifications, methylation, phosphorylation, and proteomics.

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“…Numerous mouse models have been established as a genetic reference population (GRP) but they all derive from inbred lines, giving a limited genetic diversity. However, a new resource, the Collaborative Cross (CC) population, has been developed, which provides a unique and sufficiently powerful resource for genetic studies 12–15 . Because of its multi‐parental inbreeding, it offers fourfold map expansion, increasing the accuracy of QTL map location 16–18 .…”

Section: Introductionmentioning

confidence: 99%

“…However, a new resource, the Collaborative Cross (CC) population, has been developed, which provides a unique and sufficiently powerful resource for genetic studies. 12 , 13 , 14 , 15 Because of its multi‐parental inbreeding, it offers fourfold map expansion, increasing the accuracy of QTL map location. 16 , 17 , 18 Moreover, because of their inbred nature, all mice within a generated CC line are homozygotes and genetically identical, 17 , 19 , 20 providing an ideal model to explore bimolecular networks and systems‐level phenotypes underlying complex traits.…”

Section: Introductionmentioning

confidence: 99%

“… 25 Combining the whole genome sequence data of the eight founders of the CC population 26 with the advantage of the merge analysis, 21 it is possible to refine the QTL map interval to a small genomic region and identify strong candidate genes. 27 , 28 , 29 , 30 , 31 , 32 Searching for candidate genes within the fine‐mapped QTL regions can reveal genes involved in T‐cell regulation.…”

Section: Introductionmentioning

confidence: 99%

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Identifying genetic susceptibility to Aspergillus fumigatus infection using collaborative cross mice and RNA‐Seq approach

Yosief,

Lone,

Nachshon

et al. 2024

Anim Models and Exp Med

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BackgroundAspergillus fumigatus (Af) is one of the most ubiquitous fungi and its infection potency is suggested to be strongly controlled by the host genetic background. The aim of this study was to search for candidate genes associated with host susceptibility to Aspergillus fumigatus (Af) using an RNAseq approach in CC lines and hepatic gene expression.MethodsWe studied 31 male mice from 25 CC lines at 8 weeks old; the mice were infected with Af. Liver tissues were extracted from these mice 5 days post‐infection, and next‐generation RNA‐sequencing (RNAseq) was performed. The GENE‐E analysis platform was used to generate a clustered heat map matrix.ResultsSignificant variation in body weight changes between CC lines was observed. Hepatic gene expression revealed 12 top prioritized candidate genes differentially expressed in resistant versus susceptible mice based on body weight changes. Interestingly, three candidate genes are located within genomic intervals of the previously mapped quantitative trait loci (QTL), including Gm16270 and Stox1 on chromosome 10 and Gm11033 on chromosome 8.ConclusionsOur findings emphasize the CC mouse model's power in fine mapping the genetic components underlying susceptibility towards Af. As a next step, eQTL analysis will be performed for our RNA‐Seq data. Suggested candidate genes from our study will be further assessed with a human cohort with aspergillosis.

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Intestinal cancer development in response to oral infection with high-fat diet-induced Type 2 diabetes (T2D) in collaborative cross mice under different host genetic background effects

Cited by 16 publications

References 47 publications

The Complexity of Skeletal Transverse Dimension: From Diagnosis, Management, and Treatment Strategies to the Application of Collaborative Cross (CC) Mouse Model

The Complexity of Skeletal Transverse Dimension: From Diagnosis, Management, and Treatment Strategies to the Application of Collaborative Cross (CC) Mouse Model

Towards system genetics analysis of head and neck squamous cell carcinoma using the mouse model, cellular platform, and clinical human data

Identifying genetic susceptibility to Aspergillus fumigatus infection using collaborative cross mice and RNA‐Seq approach

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