Intestinal Cancer

Brockhausen, Inka; Kuhns, William J.

doi:10.1007/978-3-662-21960-7_20

Cited by 1 publication

(1 citation statement)

References 89 publications

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“…The presence of O ‐acetylated sialic acid has selective and widespread distribution and is developmentally regulated (Varki, 1992; Klein et al ., 1994). Even though the biological function of these residues is still unknown, it has been demonstrated that numerous cancers are associated with a deregulation of the sialic acids O ‐acetylation (Brockenhausen and Kuhns, 1997; Klein and Roussel, 1998; Pal et al ., 2000) and that O ‐acetylation of plant cell wall polysaccharides exists (Pauly and Scheller, 2000). Interestingly, we were unable to identify any homologue in the complete S. cerevisiae genome or in other partially available ascomycete yeast genomes.…”

Section: Discussionmentioning

confidence: 99%

Cas1p is a membrane protein necessary for the O‐acetylation of the Cryptococcus neoformans capsular polysaccharide

Janbon

Himmelreich

Moyrand

et al. 2001

Molecular Microbiology

165

191

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The capsule is certainly the most obvious virulence factor for Cryptococcus neoformans. The main capsule constituents are glucuronoxylomannans (GXM). Several studies have focused on the structure and chemistry of the GXM component of the capsule, yet little is known about the genetic basis of the capsule construction. Using a monoclonal antibody specific to a sugar epitope, we isolated a capsule‐structure mutant strain and cloned by complementation a gene named CAS1 that codes for a putative membrane protein. Although no sequence homology was found with any known protein in the different databases, protein analysis using the Propsearch software classified Cas1p as a putative glycosyltransferase. Cas1p is a well‐conserved evolutionary protein, as we identified one orthologue in the human genome, one in the drosophila genome and four in the plant Arabidopsis thaliana genome. Analysis of the capsule structure after CAS1 deletion showed that it is required for GXM O‐acetylation.

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