Intestinal Barrier Function in Gluten-Related Disorders

Cardoso-Silva, Danielle; Delbue, Deborah; Itzlinger, Alice; Moerkens, Renée; Withoff, Sebo; Branchi, Federica; Schümann, Michael

doi:10.3390/nu11102325

Cited by 80 publications

(71 citation statements)

References 133 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Recently, it was suggested that chronic disruptions in the functioning of the intestinal barrier can participate in the pathogenesis of several disorders, including autoimmune diseases [ 1 , 5 , 6 ]. In the altered barrier, non-competent intercellular junctions allow antigens derived from the food or intestinal microbiota to enter the internal milieu which challenges the immune system, resulting in the upregulated immune response.…”

Section: Introductionmentioning

confidence: 99%

Intestinal Permeability in Children with Celiac Disease after the Administration of Oligofructose-Enriched Inulin into a Gluten-Free Diet—Results of a Randomized, Placebo-Controlled, Pilot Trial

2020

View full text Add to dashboard Cite

Abnormalities in the intestinal barrier are a possible cause of celiac disease (CD) development. In animal studies, the positive effect of prebiotics on the improvement of gut barrier parameters has been observed, but the results of human studies to date remain inconsistent. Therefore, this study aimed to evaluate the effect of twelve-week supplementation of a gluten-free diet (GFD) with prebiotic oligofructose-enriched inulin (10 g per day) on the intestinal permeability in children with CD treated with a GFD. A pilot, randomized, placebo-controlled nutritional intervention was conducted in 34 children with CD, being on a strict GFD. Sugar absorption test (SAT) and the concentrations of intestinal permeability markers, such as zonulin, intestinal fatty acid-binding protein, claudin-3, calprotectin, and glucagon-like peptide-2, were measured. We found that the supplementation with prebiotic did not have a substantial effect on barrier integrity. Prebiotic intake increased excretion of mannitol, which may suggest an increase in the epithelial surface. Most children in our study seem to have normal values for intestinal permeability tests before the intervention. For individuals with elevated values, improvement in calprotectin and SAT was observed after the prebiotic intake. This preliminary study suggests that prebiotics may have an impact on the intestinal barrier, but it requires confirmation in studies with more subjects with ongoing leaky gut.

show abstract

Section: Introductionmentioning

confidence: 99%

Intestinal Permeability in Children with Celiac Disease after the Administration of Oligofructose-Enriched Inulin into a Gluten-Free Diet—Results of a Randomized, Placebo-Controlled, Pilot Trial

2020

View full text Add to dashboard Cite

show abstract

“…Importantly, the barrier function is maintained by a protein-protein complex interaction, where the main structure is the tight junction (TJ) proteins. TJs are the most apical contact between enterocytes formed by integral membrane proteins, including occludin, claudins, and scaffolding proteins as ZO-1 [6]. Although structural changes in celiac barrier function can be allocated to enterocyte TJ composition and epithelial transcytosis of gliadin peptides, research aiming to clarify how these changes arise is scarce [7,8,9,10].…”

Section: Introductionmentioning

confidence: 99%

Celiac Disease Monocytes Induce a Barrier Defect in Intestinal Epithelial Cells

Delbue

Cardoso-Silva

Branchi

et al. 2019

IJMS

Self Cite

View full text Add to dashboard Cite

Intestinal epithelial barrier function in celiac disease (CeD) patients is altered. However, the mechanism underlying this effect is not fully understood. The aim of the current study was to evaluate the role of monocytes in eliciting the epithelial barrier defect in CeD. For this purpose, human monocytes were isolated from peripheral blood mononuclear cells (PBMCs) from active and inactive CeD patients and healthy controls. PBMCs were sorted for expression of CD14 and co-cultured with intestinal epithelial cells (IECs, Caco2BBe). Barrier function, as well as tight junctional alterations, were determined. Monocytes were characterized by profiling of cytokines and surface marker expression. Transepithelial resistance was found to be decreased only in IECs that had been exposed to celiac monocytes. In line with this, tight junctional alterations were found by confocal laser scanning microscopy and Western blotting of ZO-1, occludin, and claudin-5. Analysis of cytokine concentrations in monocyte supernatants revealed higher expression of interleukin-6 and MCP-1 in celiac monocytes. However, surface marker expression, as analyzed by FACS analysis after immunostaining, did not reveal significant alterations in celiac monocytes. In conclusion, CeD peripheral monocytes reveal an intrinsically elevated pro-inflammatory cytokine pattern that is associated with the potential of peripheral monocytes to affect barrier function by altering TJ composition.

show abstract

“…Recent evidence has suggested that, despite a lifelong gluten-free diet, some disorders can persist, e.g., increased gut permeability, small bowel overgrowth, microbiota changes, exocrine pancreatic insufficiency, and low-grade gastrointestinal inflammation [46][47][48][49]. Some of these disorders could be responsible for the development of NAFLD.…”

Section: Discussionmentioning

confidence: 99%

Celiac Disease, Gluten-Free Diet, and Metabolic and Liver Disorders

et al. 2020

View full text Add to dashboard Cite

Celiac disease (CD) is a chronic autoimmune enteropathy triggered by the ingestion of gluten in genetically predisposed individuals. At the time of diagnosis, the frequency of nonalcoholic fatty liver disease (NAFLD) and nonalcoholic steatohepatitis in individuals with CD appears to be similar to that of the general population, although a lower body mass index and a lower rate of hypercholesterolemia and type 2 diabetes mellitus are observed at diagnosis in CD patients. The effect of a gluten-free diet (GFD) in individuals with these liver and metabolic disorders is still a matter of debate. The aim of this study was to investigate the links between a GFD and metabolic/liver disorders in CD patients. A systematic electronic search of the literature from January 2009 to December 2019 was performed using Medline, Web of Science, Scopus, and the Cochrane Library. Only papers written in English concerning metabolic and liver disorders in adult patients with CD were included. Out of 1195 citations, 14 eligible studies were identified. Increases in the frequency of NAFLD, weight gain, and alterations of the lipid profile suggest that important changes happen in celiac patients on a GFD, though the physiopathology of these conditions is unclear. Although a GFD is the only effective treatment available for CD, liver function, body weight, and metabolic and nutritional profiles should be monitored in patients on a GFD.

show abstract

Intestinal Barrier Function in Gluten-Related Disorders

Cited by 80 publications

References 133 publications

Intestinal Permeability in Children with Celiac Disease after the Administration of Oligofructose-Enriched Inulin into a Gluten-Free Diet—Results of a Randomized, Placebo-Controlled, Pilot Trial

Intestinal Permeability in Children with Celiac Disease after the Administration of Oligofructose-Enriched Inulin into a Gluten-Free Diet—Results of a Randomized, Placebo-Controlled, Pilot Trial

Celiac Disease Monocytes Induce a Barrier Defect in Intestinal Epithelial Cells

Celiac Disease, Gluten-Free Diet, and Metabolic and Liver Disorders

Contact Info

Product

Resources

About