Intestinal  anti-inflammatory effects of RGD-functionalized silk fibroin nanoparticles in trinitrobenzenesulfonic acid-induced experimental colitis in rats

Rodríguez-Nogales, Alba; Algieri, Francesca; Matteis, Laura De; Lozano-Pérez, Antonio Abel; Garrido-Mesa, José; Vezza, Teresa; Fuente, Jesús M. de la; Cenis, J. L.; Gálvez, Júlio; Rodríguez-Cabezas, M. Elena

doi:10.2147/ijn.s116479

Cited by 44 publications

(24 citation statements)

References 48 publications

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“…This makes them cost effective. Among various adhesive-associated peptides, RGD is commonly applied in tissue regeneration, coating chitosan 51 , fibroin 52 , titanium 53 , and so forth. In the field of peripheral nerve injury, some literature has mentioned its advantageous role in nerve repair.…”

Section: Discussionmentioning

confidence: 99%

An integrated multi-layer 3D-fabrication of PDA/RGD coated graphene loaded PCL nanoscaffold for peripheral nerve restoration

et al. 2018

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As a conductive nanomaterial, graphene has huge potentials in nerve function restoration by promoting electrical signal transduction and metabolic activities with unique topological properties. Polydopamine (PDA) and arginylglycylaspartic acid (RGD) can improve cell adhesion in tissue engineering. Here we report an integrated 3D printing and layer-by-layer casting (LBLC) method in multi-layered porous scaffold fabrication. The scaffold is composed of single-layered graphene (SG) or multi-layered graphene (MG) and polycaprolactone (PCL). The electrically conductive 3D graphene scaffold can significantly improve neural expression both in vitro and in vivo. It promotes successful axonal regrowth and remyelination after peripheral nerve injury. These findings implicate that graphene-based nanotechnology have great potentials in peripheral nerve restoration in preclinical and clinical application.

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Section: Discussionmentioning

confidence: 99%

An integrated multi-layer 3D-fabrication of PDA/RGD coated graphene loaded PCL nanoscaffold for peripheral nerve restoration

et al. 2018

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“…In fact, different authors demonstrated that the use of silk nanoparticles, as anticancer drug delivery systems, can increase the drug accumulation in target cancer tissues and reduce the efflux pump-mediated drug resistance [7,48]. Furthermore, silk fibroin nanoparticles can be functionalized, with the peptide motif RGD (arginine-glycine-aspartic acid), to enhance their interaction with tumor tissues and to reduce side effects [49,50]. Other experiments will be performed to optimize the exogenous drug loading procedure with the final aim to improve the drug loading and the loading efficiency.…”

Section: Discussionmentioning

confidence: 99%

Paclitaxel-Loaded Silk Fibroin Nanoparticles: Method Validation by UHPLC-MS/MS to Assess an Exogenous Approach to Load Cytotoxic Drugs

et al. 2019

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The aim of this work was to load an anticancer drug, paclitaxel (PTX), on Silk Fibroin Nanoparticles (SFNs) by using an exogenous approach. SFNs were produced, freeze-dried and then loaded with PTX. An exogenous method allowed us to reduce both drug loss and environmental impact. In order to quantify PTX loaded in SFNs, a simple and reliable method using reversed phase liquid chromatography coupled to tandem mass spectrometry (rp-UHPLC-MS/MS) was developed. This methodology was validated by the determination of spiked QC samples in three consecutive days. Good accuracy and precision of the method were obtained, while the intra-day and inter-day precisions were less than 10.3%. For PTX, the limit of quantitation (LOQ) was 5.0 ng/mL. Recovery from the matrix (SFNs-PTX pellets) was calculated (81.2% at LOQ value) as PTX was entrapped in a new matrix like the polymer silk fibroin-based. This method was successfully applied to determine the encapsulation efficiency (1.00 ± 0.19%) and the nanoparticle loading (0.12 ± 0.02% w/w). The in vitro anticancer activity of SFNs-PTX was tested against CFPAC-1 cancer cells; results demonstrated a very high cytotoxic activity of SFNs-PTX, with a dose dependent inhibition of CFPAC-1 proliferation, confirmed by the IC50 value of 3450 ± 750 ng/mL.

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“…Triple-bond-functionalized nanoparticles (FNPs) were prepared by performing a condensation reaction between an NHS-activated linker bearing a terminal alkyne moiety and SFNs (for details, see Text S1 ). Lysine residue on the fibroin’s surface, 80 nmol/mg of protein on average [ 17 ], was chosen as the linker’s anchoring point through an amide bond formation. The obtained FNPs were then subjected to dialysis to remove all non-covalently linked small molecules (cut-off 3–5 kDa MWCO).…”

Section: Methodsmentioning

confidence: 99%

“…The coating of nanoparticles with integrin ligands has been explored to guide cytotoxic reagents directly to the cancer cell surface [ 17 , 18 ]. In this regard, the tripeptide motif Arg-Gly-Asp (RGD) is well-known for being recognized by the integrin superfamily members.…”

Section: Introductionmentioning

confidence: 99%

Silk Fibroin Nanoparticle Functionalization with Arg-Gly-Asp Cyclopentapeptide Promotes Active Targeting for Tumor Site-Specific Delivery

Bari

Serra

Paolillo

et al. 2021

Cancers

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Arg-Gly-Asp (RGD)-based cyclopentapeptides (cRGDs) have a high affinity towards integrin αvβ3 and αvβ5, which are overexpressed by many tumor cells. Here, curcumin-loaded silk fibroin nanoparticles (SFNs) have been functionalized on the surface with cRGD to provide active targeting towards tumor cells; a “click reaction” between the RGD-based cyclopentapeptide carrying an azide group and triple-bond-functionalized nanoparticles has been exploited. Both naked and functionalized SFNs were less than 200 nm in diameter and showed a round-shaped morphology but, after functionalization, SFNs increased in size and protein molecular weight. The functionalization of SFNs’ surfaces with cRGD provided active internalization by cells overexpressing integrin receptors. At the lowest concentration tested (0.01 mg/mL), functionalized SFNs showed more effective uptake with respect to the naked by tumor cells that overexpress integrin receptors (but not for non-overexpressing ones). In contrast, at higher concentrations, the non-specific cell membrane protein–particle interactions are promoted and coupled to specific and target mediated uptake. Visual observations by fluorescence microscopy suggested that SFNs bind to integrin receptors on the cell surface and are then internalized by endocytosis. Overall, SFN functionalization provided in vitro active targeting for site-specific delivery of anticancer drugs, boosting activity and sparing healthy organs.

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Intestinal anti-inflammatory effects of RGD-functionalized silk fibroin nanoparticles in trinitrobenzenesulfonic acid-induced experimental colitis in rats

Cited by 44 publications

References 48 publications

An integrated multi-layer 3D-fabrication of PDA/RGD coated graphene loaded PCL nanoscaffold for peripheral nerve restoration

An integrated multi-layer 3D-fabrication of PDA/RGD coated graphene loaded PCL nanoscaffold for peripheral nerve restoration

Paclitaxel-Loaded Silk Fibroin Nanoparticles: Method Validation by UHPLC-MS/MS to Assess an Exogenous Approach to Load Cytotoxic Drugs

Silk Fibroin Nanoparticle Functionalization with Arg-Gly-Asp Cyclopentapeptide Promotes Active Targeting for Tumor Site-Specific Delivery

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