Intestinal amino acid and peptide transporters in broiler are modulated by dietary amino acids and protein

Osmanyan, A.K.; Harsini, Shahab Ghazi; Mahdavi, Reza; Фисинин, В.И.; Arkhipova, A.L.; Glazko, Tatiana Teodorovna; Kovalchuk, Svetlana N.; Kosovsky, G.Yu.

doi:10.1007/s00726-017-2510-6

Cited by 39 publications

(23 citation statements)

References 22 publications

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“…Even when an adequate amount of calories is supplied in the blood, as for instance with parenteral nutrition, morphological and functional alterations of the small intestine mucosa are observed; the small intestine absorptive surface area appears to be reduced because of a decrease in cell proliferation, differentiation, and migration along the crypt-to-villi axis. That luminal amino acid availability also affects intestinal amino acid transporter expression has been shown in different animal models (2,20,27,28). In our study, we observed a reduction in cryptal cell proliferation in the intestinal area distal to the obstruction not receiving amniotic fluid.…”

Section: Discussionsupporting

confidence: 76%

Intestinal IMINO transporter SIT1 is not expressed in human newborns

Meier

Camargo

Hunziker

et al. 2018

American Journal of Physiology-Gastrointestinal and Liver Physi

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The expression of amino acid transporters in small intestine epithelium of human newborns has not been studied yet. It is further not known whether the maturation of imino acid (proline) transport is delayed as in kidney proximal tubule. The possibility to obtain small intestinal tissue from patients undergoing surgery for jejunal or ileal atresia during their first days after birth was used to address these questions. As control, adult terminal ileum tissue was sampled during routine endoscopies. Gene expression of luminal imino- and amino acid transporter SIT1 (SLC6A20) was approximately 3-fold lower in newborns versus adults. mRNA levels of all other luminal and basolateral amino acid transporters and accessory proteins tested were similar in newborn mucosa compared to adults. At the protein level, the major luminal neutral amino acid transporter BAT1 (SLC6A19) and its accessory protein angiotensin-converting enzyme 2 (ACE2) were shown by immunofluorescence to be expressed similarly in newborns and in adults. SIT1 protein was not detectable in the small intestine of human newborns, in contrast to adults. The morphology of newborn intestinal mucosa proximal and distal to the obstruction was generally normal but a decreased proliferation rate was visualized distally of the atresia by lower levels of the mitosis marker Ki-67. The mRNA level of the 13 tested amino acid transporters and accessory proteins was nontheless similar, suggesting that the intestinal obstruction and interruption of amniotic fluid passage through the small intestinal lumen did not affect amino acid transporter expression.

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Section: Discussionsupporting

confidence: 76%

Intestinal IMINO transporter SIT1 is not expressed in human newborns

Meier

Camargo

Hunziker

et al. 2018

American Journal of Physiology-Gastrointestinal and Liver Physi

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“…On the contrary, some food compounds could alter the absorption of dipeptides by adjusting the expression of PepT1. It has been reported that dietary amino acids and protein (hydrolysates) could up-regulate the expression of intestinal PepT1 mRNA to increase the intestinal transporter capacity of PepT1 and facilitate the absorption of peptides (Shiraga et al, 1999;Osmanyan et al, 2018). Meanwhile, theaflavins, polymeric catechins, have been reported to down-regulate the expression of PepT1 via the activation of intracellular AMP-dependent kinase, thus restricting the transport of dipeptides (Takeda et al, 2013).…”

Section: Factors Affecting the Absorption Of Small Peptidesmentioning

confidence: 99%

Intestinal absorption of small peptides: a review

Shen

Matsui

2018

Int J of Food Sci Tech

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Summary Peptides display diverse structural features because of their varied amino acid compositions. The structural diversity often imparts them complex physiological functions, or possible health‐beneficial effects. Some small peptides (di‐/tripeptides) exert preventive effects against conditions such as hypertension, hypercholesterolaemia and atherosclerosis. Despite their health benefits, a limited understanding of peptide absorption may hinder their extensive application. Therefore, this review briefly introduces the in vitro and in vivo findings on the intestinal absorption of small peptides and potential factors affecting their absorption.

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“…Studies have revealed that acute undernutrition up-regulates the mRNA expression of PepT1 (Sampaio et al, 2016). Furthermore, a greater amount of PepT1 and EAAT3 mRNA has been associated with the poor dietary protein quality (Osmanyan et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Gut health and serum growth hormone levels of broiler chickens fed dietary chitin and chitosan from cricket and shrimp

et al. 2019

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Growth hormones (GH) alone does not explain the growth rate in the chicken as growth in an animal is multi-factorial. Normal morphology of the intestinal villus and crypt, with adequate regulation of intestinal nutrient transporters, is essential to a healthy gut. Nutrition plays a significant role in gut health management, but information on the effect of dietary chitin and chitosan on gut morphology, gene expression of nutrient transporter, and serum levels of GH in broiler chickens is scanty. Thus, this study aimed at evaluating the comparative effect of dietary chitin and chitosan from cricket and shrimp on the small intestinal morphology, relative gene expression of intestinal nutrient transporters and serum level of GH in the broiler. A total of 150 day-old male Cobb500 broiler chicks were randomly allotted to one of the five treatment groups (n = 30). Treatment 1 was fed basal diet only, treatments 2 to 5 were fed a basal diet with 0.5 g cricket chitin, cricket chitosan, shrimp chitin, and shrimp chitosan, respectively, per kg diet. At days 21 and 42, duodenal and jejunal samples were assessed for structural morphology and jejunum for the relative gene expression of PepT1, EAAT3, SGLT1, and SGLT5 using quantitative real-time PCR. Results bared that dietary cricket chitosan and shrimp chitosan significantly (P < 0.05) improved jejunal villus height and reduced crypt depth without improving the body weight (BW). The gut morphology of birds under cricket chitin was poor and significantly (P < 0.05) different from other treated groups. Both the dietary chitin and chitosan at day 21 and only dietary chitosan at day 42 significantly (P < 0.05) down-regulated the relative mRNA expression of PepT1, EAAT3, SGLT1, and SGLT5 of broiler chickens. Treated groups differ non-significantly at both phases, while cricket chitin numerically increased the relative expression of PepT1, EAAT3, and SGLT1. Therefore, the potential of cricket chitin to improve BW and to up-regulate nutrient transporters is worthy of further exploration.

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Intestinal amino acid and peptide transporters in broiler are modulated by dietary amino acids and protein

Cited by 39 publications

References 22 publications

Intestinal IMINO transporter SIT1 is not expressed in human newborns

Intestinal IMINO transporter SIT1 is not expressed in human newborns

Intestinal absorption of small peptides: a review

Gut health and serum growth hormone levels of broiler chickens fed dietary chitin and chitosan from cricket and shrimp

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