Intervertebral disc regeneration: a great challenge for tissue engineers

Colombier, Pauline; Camus, Anne; Lescaudron, Laurent; Clouet, Johann; Guicheux, Jérôme

doi:10.1016/j.tibtech.2014.05.006

Cited by 40 publications

(29 citation statements)

References 15 publications

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“…2004, Colombier et al. 2014). Nucleopulpocytes are the effector cells of the NP, which are responsible for the synthesis of extracellular matrix (ECM) components, while NTCs are considered to be the NP progenitors, maintaining homeostasis (Aguiar et al.…”

Section: Intervertebral Discsmentioning

confidence: 99%

Ageing in the musculoskeletal system

Roberts¹,

Colombier²,

Sowman³

et al. 2016

Acta Orthopaedica

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The extent of ageing in the musculoskeletal system during the life course affects the quality and length of life. Loss of bone, degraded articular cartilage, and degenerate, narrowed intervertebral discs are primary features of an ageing skeleton, and together they contribute to pain and loss of mobility. This review covers the cellular constituents that make up some key components of the musculoskeletal system and summarizes discussion from the 2015 Aarhus Regenerative Orthopaedic Symposium (AROS) (Regeneration in the Ageing Population) about how each particular cell type alters within the ageing skeletal microenvironment.

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Section: Intervertebral Discsmentioning

confidence: 99%

Ageing in the musculoskeletal system

Roberts¹,

Colombier²,

Sowman³

et al. 2016

Acta Orthopaedica

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“…There are extensive reports about the pathogenesis of spinal degeneration and the primary therapies currently in use. These include Western medicine, surgical operations, and IVD tissue engineering [11–13]. However, there are few reports on the effect of traditional Chinese medicines, such as icariin, on NP and annulus fibrosus cells, although, as an old Chinese traditional medicine, icariin has been reported to benefit osteogenesis in vivo [14], accelerate the differentiation of osteoblast and mesenchymal stem cells [15], and protect neurocytes [16].…”

Section: Discussionmentioning

confidence: 99%

Icariin Prevents H₂O₂-Induced Apoptosis via the PI3K/Akt Pathway in Rat Nucleus Pulposus Intervertebral Disc Cells

Deng

Chen

Zheng

et al. 2017

Evidence-Based Complementary and Alternative Medicine

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Icariin is a prenylated flavonol glycoside derived from the Chinese herb Epimedium sagittatum. This study investigated the mechanism by which icariin prevents H2O2-induced apoptosis in rat nucleus pulposus (NP) cells. NP cells were isolated from the rat intervertebral disc and they were divided into five groups after 3 passages: (A) blank control; (B) 200 μM H2O2; (C) 200 μM H2O2 + 20 μM icariin; (D) 20 μM icariin + 200 μM H2O2 + 25 μM LY294002; (E) 200 μM H2O2 + 25 μM LY294002. LY294002 is a selective inhibitor of the phosphoinositide 3-kinase (PI3K)/Akt signaling pathway. NP cell viability, apoptosis rate, intracellular reactive oxygen species levels, and the expression of AKT, p-AKT, p53, Bcl-2, Bax, caspase-3 were estimated. The results show that, compared with the control group, H2O2 significantly increased NP cell apoptosis and the level of intracellular ROS. Icariin pretreatment significantly decreased H2O2-induced apoptosis and intracellular ROS and upregulated p-Akt and BCL-2 and downregulated caspase-3 and Bax. LY294002 abolished the protective effects of icariin. Our results show that icariin can attenuate H2O2-induced apoptosis in rat nucleus pulposus cells and PI3K/AKT pathway is at least partly included in this protection effect.

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“…There are two different cell types in the NP: notochordal cells and mature NP cells. The latter are called chondrocyte-like cells due to their rounded shape and secretory activity, and are found primarily in adults [65]. An example of a scaffold appropriate for NP tissue engineering is a chitosan-based hydrogel seeded with IVD cells [15,66].…”

Section: Tissue Engineering Of Af and Npmentioning

confidence: 99%

“…This significantly impacted the development of tissue engineering in various medical fields, including regenerative techniques for intervertebral disc (IVD) degeneration (IDD). Degenerative discs are among the main causes of chronic segmental spinal instability in both males and females, and significantly affect the quality of life, especially of young patients [1]. IDD may present clinically as axial back pain, spinal stenosis, myelopathy, or radiculopathy [2][3][4].…”

Section: Introductionmentioning

confidence: 99%

Intervertebral disc tissue engineering: A brief review

Stergar

Gradišnik

Velnar

et al. 2019

Bosn J of Basic Med Sci

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Intervertebral disc (IVD) degeneration (IDD) is associated with low back pain and significantly affects the patient’s quality of life. Degeneration of the IVD alters disk height and the mechanics of the spine, leading to chronic segmental spinal instability. The pathophysiology of IVD disease is still not well understood. Current therapies for IDD include conservative and invasive approaches, but none of those treatments are able to restore the disc structure and function. Recently, tissue engineering techniques emerged as a possible approach to treat IDD, by replacing a damaged IVD with scaffolds and appropriate cells. Advances in manufacturing techniques, material processing and development, surface functionalization, drug delivery systems and cell incorporation furthered the development of tissue engineering therapies. In this review, biomaterial scaffolds and cell-based therapies for IVD regeneration are briefly discussed.

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Intervertebral disc regeneration: a great challenge for tissue engineers

Cited by 40 publications

References 15 publications

Ageing in the musculoskeletal system

Ageing in the musculoskeletal system

Icariin Prevents H₂O₂-Induced Apoptosis via the PI3K/Akt Pathway in Rat Nucleus Pulposus Intervertebral Disc Cells

Intervertebral disc tissue engineering: A brief review

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Intervertebral disc regeneration: a great challenge for tissue engineers

Cited by 40 publications

References 15 publications

Ageing in the musculoskeletal system

Ageing in the musculoskeletal system

Icariin Prevents H2O2-Induced Apoptosis via the PI3K/Akt Pathway in Rat Nucleus Pulposus Intervertebral Disc Cells

Intervertebral disc tissue engineering: A brief review

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Icariin Prevents H₂O₂-Induced Apoptosis via the PI3K/Akt Pathway in Rat Nucleus Pulposus Intervertebral Disc Cells