Intervention Strategies to Control Campylobacter at Different Stages of the Food Chain

Taha-Abdelaziz, Khaled; Singh, Mankerat; Sharif, Shayan; Sharma, Shreeya; Kulkarni, Raveendra R.; Alizadeh, Mohammad Ali; Yitbarek, Alexander; Helmy, Yosra A.

doi:10.3390/microorganisms11010113

Cited by 27 publications

(30 citation statements)

References 211 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Campylobacter jejuni poses a serious threat to public health and currently there are no available antibacterial solutions to eliminate this pathogen through the food production chain (20). Here, we exploit the target specificity of phages and the killing efficiency of pyocins to develop novel antibacterials against C. jejuni.…”

Section: Discussionmentioning

confidence: 99%

Campycins are novel broad spectrum antibacterials killingCampylobacter jejuniby targeting the essential major outer membrane protein (MOMP)

Zampara,

Gencay,

Brøndsted

et al. 2023

Preprint

View full text Add to dashboard Cite

Pyocins are high molecular weight bacteriocins produced byPseudomonas aeruginosathat can be retargeted to new bacterial species by exchanging the pyocin tail fibers with bacteriophage receptor binding proteins (RBPs). Here, we develop retargeted pyocins called campycins as new antibacterials to specifically and effectively kill the major foodborne pathogenCampylobacter jejuni.We used two diverse RBPs (H-fibers) encoded by CJIE1 prophages found in the genomes ofC. jejunistrains CAMSA2147 and RM1221 to construct Campycin 1 and Campycin 2, respectively. Together Campycin 1 and 2 could target allC. jejunistrains tested due to complementary antibacterial spectrums. In addition, both campycins led to more than 3 log reductions inC. jejunicounts under microaerobic conditions at 42°C, whereas the killing efficiency was less efficient under anaerobic conditions at 5°C. We furthermore discovered that both H-fibers used to construct the campycins bind to the essential major outer membrane protein (MOMP) present in allC. jejuni,in a strain specific manner. Protein sequence alignment and structural modelling suggest that the highly variable extracellular loops of MOMP form the binding sites of the diverse H-fibers. Furtherin silicoanalyses of 5000 MOMP sequences suggest that the protein fall into three major clades predicted to be targeted by either Campycin 1 or Campycin 2. Thus, campycins are promising antibacterials againstC. jejuniexpected to broadly target numerous strains of this human pathogen found in nature and agriculture.IMPORTANCECampylobacter jejuniis the leading cause of bacterial foodborne gastroenteritis and responsible for more than 800 million cases globally each year posing a continuous risk to human health and a huge economic and societal burden. Here, we developed re-targeted R2 pyocins (campycins) as novel antibacterials againstC. jejuniby using the receptor binding proteins of CJIE1 prophages observed in manyC. jejunigenomes. Notably, campycins broadly target the highly variable yet essential major outer membrane protein (MOMP), and result in more than 3-log reductions inC. jejunicounts under conditions promoting bacterial growth. We therefore propose that campycins have the potential to lowerC. jejunicolonization levels in the chicken gut, the main reservoir and cause of human disease, representing a novel efficient antibacterial solution specifically developed to target this widespread foodborne pathogen.

show abstract

Section: Discussionmentioning

confidence: 99%

Campycins are novel broad spectrum antibacterials killingCampylobacter jejuniby targeting the essential major outer membrane protein (MOMP)

Zampara,

Gencay,

Brøndsted

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…To successfully combat Campylobacter at farm level, it is necessary to prevent and impede introduction into poultry flocks, the transmission, colonization, and to reduce its overall prevalence 5 . Results of previous studies indicate that individual measures may not be sufficient to control Campylobacter 6 , 7 . For this reason, it has been suggested that a combination of multiple strategies might be beneficial 3 , 8 , 9 , a new control strategy summarized under the term “multiple-hurdle approach” .…”

Section: Introductionmentioning

confidence: 96%

Combined application of bacteriophages with a competitive exclusion culture and carvacrol with organic acids can reduce Campylobacter in primary broiler production

Peh,

Szott,

Reichelt

et al. 2024

Sci Rep

View full text Add to dashboard Cite

For reducing Campylobacter (C.) in the food production chain and thus the risk to the consumer, the combined application of different measures as a multiple-hurdle approach is currently under discussion. This is the first study to investigate possible synergistic activities in vivo, aiming at reducing intestinal C. jejuni counts by administering (i) bacteriophages (phages) in combination with a competitive exclusion (CE) product and (ii) carvacrol combined with organic acids. The combined application of the two selected phages (Fletchervirus phage NCTC 12673 and Firehammervirus phage vB_CcM-LmqsCPL1/1) and the CE product significantly reduced C. jejuni loads by 1.0 log10 in cecal and colonic contents as well as in cloacal swabs at the end of the trial (33 and 34 days post hatch). The proportion of bacterial isolates showing reduced phage susceptibility ranged from 10.9% (isolates from cecal content) to 47.8% (isolates from cloacal swabs 32 days post hatch) for the Fletchervirus phage, while all tested isolates remained susceptible to the Firehammervirus phage. The use of carvacrol combined with an organic acid blend (sorbic acid, benzoic acid, propionic acid, and acetic acid) significantly reduced Campylobacter counts by 1.0 log10 in cloacal swabs on day 30 only.

show abstract

“…Preharvest control of Campylobacter in poultry production is a critical step in order to reduce carcass contaminations during processing and subsequent foodborne transmission [24,25]. However, poultry houses can be contaminated by Campylobacter in many different ways, making controlling Campylobacter in poultry a very challenging task [20].…”

Section: Introductionmentioning

confidence: 99%

“…Multiple non-biosecurity measures (e.g., vaccines, bacteriocins, phages, feed additives, competitive exclusion products, etc.) have been evaluated to control Campylobacter in live birds with various outcomes [20,24,25,27,28]. Significant reductions in Campylobacter colonization in chickens were observed in several studies involving bacteriophages, various types of vaccines, and feed additives (29,30,31,32,33).…”

Section: Introductionmentioning

confidence: 99%

“…Inoculating young broilers with fecal microbiota transplants from highly feedefficient donors was shown to affect the cecal microbiota composition and the host's intestinal development [38]. Moreover, various degrees of success have been observed in the control of enteric foodborne bacteria (e.g., Salmonella and Campylobacter) in the poultry intestinal tract among different studies employing FMT [24,[39][40][41][42][43][44].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Fecal Microbiota Transplantation Reduces Campylobacter jejuni Colonization in Young Broiler Chickens Challenged by Oral Gavage but Not by Seeder Birds

Pang,

Beyi,

Looft

et al. 2023

Antibiotics

View full text Add to dashboard Cite

Campylobacter spp., particularly C. jejuni and C. coli, are major food safety concerns, transmitted to humans mainly via contaminated poultry meat. In a previous study, we found that some commercial broiler farms consistently produced Campylobacter-free flocks while others consistently reared Campylobacter-colonized flocks, and significant differences in the gut microbiota compositions between the two types of farm categories were revealed. Therefore, we hypothesized that gut microbiota influences Campylobacter colonization in poultry and that the microbiota from Campylobacter-free flocks may confer colonization resistance to Campylobacter in the chicken intestine. In this study, two fecal microbiota transplantation (FMT) trials were performed to test the hypothesis. Newly hatched chicks were given FMT via oral gavage of the cecal content of Campylobacter-free adult chickens (treatment groups) or PBS (control groups) before the feed consumption. Approximately two weeks after the FMT, the birds were challenged with C. jejuni either by oral gavage (trial 1) or by co-mingling with Campylobacter-colonized seeder birds (trial 2) to evaluate the potential protective effect of the FMT. Cecal contents were collected (3 times, 5 days apart) to determine the Campylobacter colonization levels via culture and microbiota compositions via 16S rRNA gene sequencing. FMT reduced cecal Campylobacter colonization significantly (log10 1.2–2.54 CFU/g) in trial 1 but not in trial 2, although FMT significantly impacted the diversity and compositions of the gut microbiota in both trials. Several genera, such as Butyricimonas, Parabacteroides, Parasutterella, Bilophila, Fournierella, Phascolarctobacterium, and Helicobacter, had increased abundance in the FMT-treated groups in both trials. Furthermore, Campylobacter abundance was found to be negatively correlated with the Escherichia and Ruminococcus_torques_group genera. These findings indicate that even though FMT with adult cecal microbiota can positively affect the subsequent development of the gut microbiota in young broilers, its inhibitory effect on Campylobacter colonization varies and appears to be influenced by the challenge models.

show abstract

Intervention Strategies to Control Campylobacter at Different Stages of the Food Chain

Cited by 27 publications

References 211 publications

Campycins are novel broad spectrum antibacterials killingCampylobacter jejuniby targeting the essential major outer membrane protein (MOMP)

Campycins are novel broad spectrum antibacterials killingCampylobacter jejuniby targeting the essential major outer membrane protein (MOMP)

Combined application of bacteriophages with a competitive exclusion culture and carvacrol with organic acids can reduce Campylobacter in primary broiler production

Fecal Microbiota Transplantation Reduces Campylobacter jejuni Colonization in Young Broiler Chickens Challenged by Oral Gavage but Not by Seeder Birds

Contact Info

Product

Resources

About