Intervention effect of Qi-Yu-San-Long Decoction on Lewis lung carcinoma in C57BL/6 mice: Insights from UPLC–QTOF/MS-based metabolic profiling

Wu, Huan; Yang, Chen; Li, Qinglin; Gao, Yating; Zhang, Xingxing; Tong, Jiabing; Zhang, Zhiqiang; Hu, Jian; Wang, Danyang; Zeng, Shi‐Jie; Li, Zegeng

doi:10.1016/j.jchromb.2018.10.013

Cited by 25 publications

(14 citation statements)

References 34 publications

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“…e QYSL formula has noticeable effects on the prevention and treatment of NSCLC [12,13]. Our previous studies showed that the anticancer properties of the QYSL formula may be associated with autophagy although the specific mechanism was not yet clear.…”

Section: Discussionmentioning

confidence: 99%

“…e formula consists of ten Chinese medicinal herbs: Astragalus, Solanum nigrum, Gecko, Earthwormg, Euphorbia helioscopia, Hedyotis diffusa Willd, Curcuma zedoaria, Coix lacryma, Polygonatum, and Fritillaria cirrhosa [12]. e untargeted metabonomics method based on UPLC-QTOF/MS has been used to screen out 21 biomarkers that may contribute to the treatment of lung cancer with the QYSL formula; the biomarkers were found to be mainly involved in sphingolipid and glycerophospholipid metabolism and fatty acid degradation [13]. Previous animal experiments have shown that the QYSL formula has tumor-suppressive properties and can decrease the expression of mTOR, a key protein in the PI3K/ Akt/mTOR signaling pathway that is involved in autophagy, suggesting that QYSL's anticancer effect may be related to the promotion of autophagy in lung cancer cells [14].…”

Section: Introductionmentioning

confidence: 99%

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Qiyusanlong Formula Induces Autophagy in Non-Small-Cell Lung Cancer Cells and Xenografts through the mTOR Signaling Pathway

Gao

Wang

Yang

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Objective. Qiyusanlong (QYSL) formula has been used in the clinic for more than 20 years and has been proved to have pronounced efficacy in the treatment of non-small-cell lung cancer (NSCLC). This work aims to evaluate the molecular mechanism of QYSL formula action on NSCLC, specifically in relation to autophagy induction. Methods. In vitro, CCK-8 was used to detect the effect of QYSL serum on cell viability in A549 cells. In vivo, A549 cells were implanted subcutaneously in nude mice to establish a xenograft model. TUNEL staining was used to measure cell apoptosis and TEM to observe the autophagy-related morphological changes in vitro and in vivo. Western blotting, RT-qPCR, and immunofluorescence were used to measure autophagy-related proteins. In addition, rapamycin (an inhibitor of mTOR and inducer of autophagy) and MHY1485 (an activator of mTOR and inhibitor of autophagy) were used to determine whether QYSL-induced autophagy was regulated by the mTOR pathway. Results. QYSL serum inhibited the cell viability of A549 cells in a concentration‐dependent manner. In vivo, the QYSL formula inhibited xenograft growth. The QYSL formula promoted apoptosis in A549 cells and induced autophagosome formation in vitro and in vivo. In addition, the QYSL formula downregulated the expression of mTOR and p62, while it upregulated the expression of ATG-7 and Beclin-1 and increased the LC3-II/LC3-I ratio. QYSL serum inhibited p-mTOR in a similar manner to rapamycin while reducing the activating effects of MHY1485 on p-mTOR. Conclusion. The QYSL formula has anti-lung cancer effects and promotes autophagy through the mTOR signaling pathway.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Qiyusanlong Formula Induces Autophagy in Non-Small-Cell Lung Cancer Cells and Xenografts through the mTOR Signaling Pathway

Gao

Wang

Yang

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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“…Qi-Yu-San-Long Decoction (QYSLD) is a classic formula, which has been widely used clinically for LLC treatment. To characterize in detail the action mechanism of how it works, a UPLC/Q-TOF MS-based metabolomics was conducted [69]. Lewis LLC mice model was firstly built, and plasma Chinese Medicines for Cancer Treatment from the Metabolomics Perspective DOI: http://dx.doi.org /10.5772/intechopen.88924 was collected for metabolomics analysis.…”

Section: Lipid Metabolismmentioning

confidence: 99%

Chinese Medicines for Cancer Treatment from the Metabolomics Perspective

Guo¹,

Tan²,

Wang³

et al. 2020

Metabolomics - New Insights Into Biology and Medicine

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Cancer is one of the most prevalent diseases all over the world with poor prognosis and the development of novel therapeutic strategies is still urgently needed. The large amount of successful experiences in fighting against cancer-like diseases with Chinese medicine has suggested it as a great source of alternative treatments to human cancers. Cancer cells have been shown to own a predominantly unique metabolic phenotype to facilitate their rapid proliferation. Metabolic reprogramming is a remarkable hallmark of cancer and therapies targeting cancer metabolism can be highly specific and effective. Based on the sophisticated study of small molecule metabolites, metabolomics can provide us valuable information on dynamically metabolic responses of living systems to certain environmental condition. In this chapter, we systematically reviewed recent studies on metabolism-targeting anticancer therapies based on metabolomics in terms of glucose, lipid, amino acid, and nucleotide metabolisms and other altered metabolisms, with special emphasis on the potential of metabolic treatment with pure compounds, herb extracts, and formulations from Chinese medicines. The trends of future development of metabolism-targeting anticancer therapies were also discussed. Overall, the elucidation of the underlying molecular mechanism of metabolism-targeting pharmacologic therapies will provide us a new insight to develop novel therapeutics for cancer treatment.

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“…The metabonomics method could provide a global overview of multiple biochemical pathways to uncover disease pathogenesis and mechanisms of multicomponent medicinal effects. Nowadays, increasing attention has been focused on metabonomics to successfully reveal the mechanisms of TCM prescriptions ( Wang et al, 2010 ; Patti et al, 2012 ; Cui et al, 2017 ; Gao et al, 2018 ; Pang et al, 2018 ; Wu et al, 2018 ; Wang et al, 2020 ; Li et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Deciphering the Underlying Mechanisms of Formula Le-Cao-Shi Against Liver Injuries by Integrating Network Pharmacology, Metabonomics, and Experimental Validation

et al. 2022

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Le-Cao-Shi (LCS) has long been used as a folk traditional Chinese medicine formula against liver injuries, whereas its pharmacological mechanisms remain elusive. Our study aims to investigate the underlying mechanism of LCS in treating liver injuries via integrated network pharmacology, metabonomics, and experimental validation. By network pharmacology, 57 compounds were screened as candidate compounds based on ADME parameters from the LCS compound bank (213 compounds collected from the literature of three single herbs). According to online compound–target databases, the aforementioned candidate compounds were predicted to target 87 potential targets related to liver injuries. More than 15 pathways connected with these potential targets were considered vital pathways in collectively modulating liver injuries, which were found to be relevant to cancer, xenobiotic metabolism by cytochrome P450 enzymes, bile secretion, inflammation, and antioxidation. Metabonomics analysis by using the supernatant of the rat liver homogenate with UPLC-Q-TOF/MS demonstrated that 18 potential biomarkers could be regulated by LCS, which was closely related to linoleic acid metabolism, glutathione metabolism, cysteine and methionine metabolism, and glycerophospholipid metabolism pathways. Linoleic acid metabolism and glutathione metabolism pathways were two key common pathways in both network pharmacology and metabonomics analysis. In ELISA experiments with the CCl4-induced rat liver injury model, LCS was found to significantly reduce the levels of inflammatory parameters, decrease liver malondialdehyde (MDA) levels, and enhance the activities of hepatic antioxidant enzymes, which validated that LCS could inhibit liver injuries through anti-inflammatory property and by suppressing lipid peroxidation and improving the antioxidant defense system. Our work could provide new insights into the underlying pharmacological mechanisms of LCS against liver injuries, which is beneficial for its further investigation and modernization.

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Intervention effect of Qi-Yu-San-Long Decoction on Lewis lung carcinoma in C57BL/6 mice: Insights from UPLC–QTOF/MS-based metabolic profiling

Cited by 25 publications

References 34 publications

Qiyusanlong Formula Induces Autophagy in Non-Small-Cell Lung Cancer Cells and Xenografts through the mTOR Signaling Pathway

Qiyusanlong Formula Induces Autophagy in Non-Small-Cell Lung Cancer Cells and Xenografts through the mTOR Signaling Pathway

Chinese Medicines for Cancer Treatment from the Metabolomics Perspective

Deciphering the Underlying Mechanisms of Formula Le-Cao-Shi Against Liver Injuries by Integrating Network Pharmacology, Metabonomics, and Experimental Validation

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