Interstrain differences of ionotropic glutamate receptor subunits in the hippocampus and induction of hippocampal sclerosis with pilocarpine in mice

Dobó, Endre; Török, I; Mihály, András; Károly, Norbert; Krisztin-Péva, Beáta

doi:10.1016/j.jchemneu.2015.02.002

Cited by 3 publications

(40 citation statements)

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“…Previously, different neurochemical profiles have been highlighted, which could account for different behavioral responses between strains (29). For example, basal levels of ionotropic glutamate receptor subunit vary according to mouse strain (30). Such diversity might also be present in the neural retina and could induce small deviations in terms of retinal visual processing among non-pathological mouse strains.…”

Section: Discussionmentioning

confidence: 99%

Maturation of the Pupil Light Reflex Occurs Until Adulthood in Mice

et al. 2019

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With respect to photoreceptor function, it is well known that electroretinogram (ERG) amplitudes decrease with age, but to our knowledge, studies describing age-related changes in the pupil light response (PLR) of mice are lacking. This study recorded the PLR and ERG in C57BL/6 and Sv129S6 wild-type mice at three different ages during early adulthood. Dark- and light-adapted PLR and ERG measurements were performed at 1, 2, and 4 months of age. For PLR measurements, we used either a red (622 nm) or blue (463 nm) light stimulus (500 ms) to stimulate one eye. We selected various light intensities ranging across almost 4 log units and subsequently classified them as low, medium, or high intensity. From the recorded PLR, we selected parameters to quantify the early and late phases of the response such as the baseline pupil size, the maximal constriction amplitude, the maximal velocity, the early partial dilation (area under the curve of the positive peak of the first derivative of PLR tracing), and the sustained constriction amplitude. For ERG measurements, both scotopic and photopic responses were recorded following stimulation with green light (520 nm) at preselected intensities. The amplitudes and latencies of the a-wave and the b-wave were also analyzed. In both strains, 1-month-old animals presented with a smaller baseline pupil diameter compared to that in 2- and 4-month-old mice. They also exhibited greater maximal constriction amplitude in response to red stimuli of medium intensity. Further, 1-month-old Sv129S6 mice responded with greater constriction amplitude to all other red and blue stimuli. One-month-old C57BL/6 mice also demonstrated faster early partial dilation and smaller sustained response to low blue stimuli. The ERG of 1-month-old C57BL/6 mice showed a greater scotopic a-wave amplitude compared to that of 2-month-old mice, whereas no significant differences were found in Sv129S6 mice. These results suggest that the functional maturation of the neuronal pathway that mediates the PLR continues after 1 month of age. In studies that measure PLR to determine retinal integrity in adult mice, it is thus important to determine normative values in animals of 2 months of age.

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Section: Discussionmentioning

confidence: 99%

Maturation of the Pupil Light Reflex Occurs Until Adulthood in Mice

et al. 2019

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“…This strong immunostaining of the SLUC in CA3 was characteristic of the histoblotting procedure, and precisely depicted the area where the mossy fibers form giant synapses (Figure 1C). This strong immunostaining of the CA3 SLUC layer has not been detected with GluK2 antibody applied on frozen sections from perfusion fixed mouse brains [15]. The immunohistochemical localization of GluK1 in histological sections of the mouse hippocampus displayed punctate immunostaining of the neuropil in the SR, and immunoreactivity of the CA3 pyramidal cell bodies and dendrites in the SLUC and SR of the CA3 (Figure 2A).…”

Section: Types Of Iglurs In the Mammalian Brainmentioning

confidence: 91%

“…The least intense staining is found in the hilus of the dentate fascia and in the SR and stratum lucidum (SLUC) of CA3 [12,13]. Frozen sections from perfusion-fixed mouse brains stained with GluA2/3 antibodies [15] have also stained several multipolar neurons in the hilus of the dentate fascia, in which the neurons were supposedly the hilar mossy cells [15,24,29].…”

Section: Types Of Iglurs In the Mammalian Brainmentioning

confidence: 99%

“…The immunohistochemical localization of GluK1 in histological sections of the mouse hippocampus displayed punctate immunostaining of the neuropil in the SR, and immunoreactivity of the CA3 pyramidal cell bodies and dendrites in the SLUC and SR of the CA3 (Figure 2A). The GluK2 antibody stained the cytoplasm of the CA3 neurons in mice [15] (Figure 2B). The strong GluK5 signal originating from the SLUC of the CA3 in histoblots (Figure 1C) suggested precise target localization, because the staining strictly corresponded to the area occupied by the mossy fiber axon terminals [13,15,16].…”

Section: Types Of Iglurs In the Mammalian Brainmentioning

confidence: 99%

“…The present review focuses on the iGluR family members, treating the possible alterations of the subunit composition of the ionotropic receptors in animal epilepsies. The author’s own results cited in this review were presented in five in extenso publications [12,13,14,15] and in one congress report [16].…”

Section: Introductionmentioning

confidence: 99%

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The Reactive Plasticity of Hippocampal Ionotropic Glutamate Receptors in Animal Epilepsies

Mihály

2019

IJMS

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Ionotropic glutamate receptors (iGluRs) mediate the synaptic and metabolic actions of glutamate. These iGluRs are classified within the α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA)-type, kainate-type, and N-methyl-d-aspartate (NMDA)-type functional receptor families. The iGluR assemblies are regulated by transcription, alternative splicing, and cytoplasmic post-translational modifications. The iGluR subunit proteins are transported from the endoplasmic reticulum, inserted into the synaptic membranes, and anchored at their action site by different scaffolding and interacting proteins. The functional properties of iGluRs depend on their subunit composition, the amino acid sequence of the protein domains, and the scaffolding proteins in the synaptic membranes. The iGluRs are removed from the membranes by enzymatic action and endocytosis. Hippocampal iGluRs are rearranged through the upregulation and downregulation of the subunits following deafferentation and epileptic seizures. The rearrangement of iGluRs and the alteration of their subunit composition transform neurons into “pathological” cells, determining the further plasticity or pathology of the hippocampal formation. In the present review, we summarize the expression of AMPA, kainate, and NMDA receptor subunits following deafferentation, repeated mild seizures, and status epilepticus. We compare our results to literature descriptions, and draw conclusions as to the reactive plasticity of iGluRs in the hippocampus.

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Hippocampal Sclerosis in Pilocarpine Epilepsy: Survival of Peptide-Containing Neurons and Learning and Memory Disturbances in the Adult NMRI Strain Mouse

Mátyás

Borbély

Mihály

2021

IJMS

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The present experiments reveal the alterations of the hippocampal neuronal populations in chronic epilepsy. The mice were injected with a single dose of pilocarpine. They had status epilepticus and spontaneously recurrent motor seizures. Three months after pilocarpine treatment, the animals were investigated with the Barnes maze to determine their learning and memory capabilities. Their hippocampi were analyzed 2 weeks later (at 3.5 months) with standard immunohistochemical methods and cell counting. Every animal displayed hippocampal sclerosis. The neuronal loss was evaluated with neuronal-N immunostaining, and the activation of the microglia was measured with Iba1 immunohistochemistry. The neuropeptide Y, parvalbumin, and calretinin immunoreactive structures were qualitatively and quantitatively analyzed in the hippocampal formation. The results were compared statistically to the results of the control mice. We detected neuronal loss and strongly activated microglia populations. Neuropeptide Y was significantly upregulated in the sprouting axons. The number of parvalbumin- and calretinin-containing interneurons decreased significantly in the Ammon’s horn and dentate gyrus. The epileptic animals displayed significantly worse learning and memory functions. We concluded that degeneration of the principal neurons, a numerical decrease of PV-containing GABAergic neurons, and strong peptidergic axonal sprouting were responsible for the loss of the hippocampal learning and memory functions.

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Interstrain differences of ionotropic glutamate receptor subunits in the hippocampus and induction of hippocampal sclerosis with pilocarpine in mice

Cited by 3 publications

References 61 publications

Maturation of the Pupil Light Reflex Occurs Until Adulthood in Mice

Maturation of the Pupil Light Reflex Occurs Until Adulthood in Mice

The Reactive Plasticity of Hippocampal Ionotropic Glutamate Receptors in Animal Epilepsies

Hippocampal Sclerosis in Pilocarpine Epilepsy: Survival of Peptide-Containing Neurons and Learning and Memory Disturbances in the Adult NMRI Strain Mouse

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