Interstitial MR lymphography in mice with gadopentetate dimeglumine and gadoxetate disodium

Abstract

Purpose: To investigate the utility of interstitial MR lymphography with gadopentetate dimeglumine (Gd-DTPA) or gadoxetate disodium (Gd-EOB-DTPA) in mice. Materials and Methods:We performed MR lymphography after the subcutaneous injection of Gd-DTPA or Gd-EOB-DTPA (0.1, 0.5, or 2.0 mmol per mouse) into the right footpad in six healthy mice, and the time courses of contrast enhancement were assessed. Additionally, the lymphatic pathways from two distinct sites were assessed in tandem by interstitial MR lymphography studies.Results: Subcutaneous injection of Gd-DTPA or Gd-EOB-DTPA caused lymph node enhancement immediately after injection, followed by a rapid decline. Dose dependency was shown for the lymph node enhancement, and a highdose injection caused prominent visualization of the veins. Lymph node enhancement did not differ significantly between Gd-DTPA and Gd-EOB-DTPA or between Gd-EOB-DTPA premixed and not premixed with bovine serum albumin. The tandem assessment of two lymphatic pathways was feasible, and image fusion aided detailed comparison. Conclusion:Interstitial MR lymphography with Gd-DTPA or Gd-EOB-DTPA allowed clear visualization of the lymphatic pathway in healthy mice, and no significant difference was found between the two agents. Their rapid kinetics limits the imaging timing window, however, facilitates repeated assessment in a single imaging session.

PEG‐poly(L‐lysine)‐based polymeric micelle MRI contrast agent: Feasibility study of a Gd‐micelle contrast agent for MR lymphography

“…For the interstitial MRL, the use of several T 1 agents have been reported. Extracellular agents such as gadopentate dimeglumine can be used for MRL, although they demonstrated nonspecific distribution and fast elimination . Micelle‐forming agents, such as gadofluorine 8 and gadofluorine P, are also used for MRL.…”

“…Extracellular agents such as gadopentate dimeglumine can be used for MRL, although they demonstrated nonspecific distribution and fast elimination. 13,14 Micelle-forming agents, such as gadofluorine 8 and gadofluorine P, are also used for MRL. They demonstrated a rather long retention to lymph node, lasting 60-90 minutes.…”

Interstitial MR Lymphography in Mice: Comparative Study with Gadofluorine 8, Gadofluorine M, and Gadofluorine P

“…11 Gadoxetate disodium, a hepatobiliary contrast agent, has high T 1 relaxivity and seems useful in MR lymphography but showed similar magnitude and temporal pattern to lymph node enhancement in a recent comparison study with the extracellular gadolinium chelate. 10 Compared to these contrast agents, the gado‰uorines have an advantage in prolonging the imaging time window.…”

“…[1][2][3][4][5][6][7][8][9][10] Contrast-enhanced sonography can survey only to a limited depth from the detector, and so, it is not suitable for detecting the deep lymphatic system. CT lymphography using water-soluble, nanoparticulate, iodine contrast, oil-in-water emulsion has been reported as a feasible method to detect lymph nodes with high resolution images.…”

“…[6][7][8][9][10] The contrast agent is injected, enters the lymphatic capillaries, and is transported with the lymphatic ‰ow to the lymph nodes, allowing functional evaluation of the lymphatic pathway as well as morphological evaluation. This method is used to detect sentinel lymph nodes.…”