Interstitial glucose and lactate balance in human skeletal muscle and adipose tissue studied by microdialysis.

Abstract

SUMMARY1. Microdialysis was used to gain insight into the substrate exchanges in the interstitial space of skeletal muscle and adipose tissue. Probes were inserted in the quadriceps femoris muscle and para-umbilical subcutaneous adipose tissue of thirteen subjects and microdialysis was performed at different flow rates (1-4 ,l min-') and during changes in tissue blood flow.2. When ethanol (5 mM) is included in the perfusion solution, the ethanol clearance from the probe is a measure of tissue blood flow. Blood flow changes induced by adenosine or vasopressin perfusion, by exercise or by circulatory occlusion resulted in ethanol clearance values of 69-139 % of the basal level. The ethanol clearance was higher in skeletal muscle than in adipose tissue (32-62 %, P< 0001), a difference compatible with a higher blood flow in muscle tissue.3. The fraction of the interstitial glucose concentration that was recovered with the microdialysis was similar in skeletal muscle (the absolute values being 1'70 + 0 14 mm at 1 #sl min-' and 059 + 005 mm at 4 #1 min-') and adipose tissue (1I89 + 020 mm at 1 pul min-'; 054 + 0 05 mm at 4 #1 min-') and correlated inversely with the tissue ethanol clearance, both in the basal state and during changes in tissue blood flow (muscle: r = -056 to -067; adipose tissue r = -072 to -095). Coefficients of variation were 6-8 % (glucose) and 11-16 % (lactate) and were similar during isometric exercise. The reproducibility of the technique (comparison of two contralateral probes; perfusion flow rate 4 ,a1 min-') was 5'3-8'3 % (ethanol) and 23-9-208 % (glucose) in muscle (n = 6) and adipose tissue (n = 4) respectively.4. The skeletal muscle dialysate lactate concentration (1 #1 min-': 1 16 + 0 2 mM) was higher than in adipose tissue (076 + 008 mm, P < 005), where the absolute amount of lactate that could be removed from the tissue (at 4 1I min-') was only half of that in skeletal muscle (0O8 + 0-11 vs. 1-76 + 023 nmol min-', P< 005

Microdialysis techniques in the study of brain and skeletal muscle

“…This issue was first raised when ethanol clearance from microdialysis perfusate was developed and validated [15] as a method of attaining a qualitative measure of local tissue blood flow at the site of microdialysis catheter insertion. Studies demonstrated that, in animals, gross changes in tissue blood flow alter the recovery of glucose by microdialysis [16] (i. e. an increase in local blood flow increases dialysate recovery of glucose) and similar findings have since been reported in human adipose and muscle tissues but with a less predictable effect of altered blood flow on the recovery of ECF lactate [17].…”

Blood flow and microdialysis in the human femoral head

“…The microdialysis catheter is estimated to drain approximately 100 mg of tissue (Rosdahl et al 1993). In bone, this is estimated to be around 100 mm 3 , while the drill hole in our study had a volume of approximately 63 mm 3 (V = πr 2 h = π × (1 mm) 2 × 20 mm).…”

Intramuscular 3-Hydroxybutyrate Levels after 60 Tetani/Min Contraction in Diabetic and Non-Diabetic Rats.

“…that substance, but also is governed by a complex interplay between cellular metabolism and the bloodstream [15]. It is, therefore, important to estimate the absolute intramuscular concentrations of 3-OHBA and lactate in diabetic and control rats by the in vivo calibration method [13] and to monitor the blood flow in contracting and noncontracting muscles [16]. These measurements were carried out in the present study.…”