Interstitial fluid pressure in rats measured with a modified wick technique

Fadnes, Hans Olav; Reed, Rolf K.; Aukland, Knut

doi:10.1016/0026-2862(77)90138-8

Cited by 177 publications

(106 citation statements)

References 19 publications

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“…IFP was measured using a modified wick-in-needle technique. 24 A sterile, 22-gauge, 6 cm spinal needle (Model 220084, Sherwood Medical Co., St. Louis, MO), with a custom-ground 2-to 4 -mm side port approximately 5 mm from the tip, was connected through 1 m of polyethylene tubing (Model PT36, Ohmeda Medical Devices Division Inc., Madison, WI) to a pressure transducer (Model P23XL, Viggo-Spectramed, Oxnard, CA) and an electronic data acquisition and recording system (Model MP100, World Precision Instruments, Sarasota, FL). The distal 1-2 cm of the needle was filled with multifilamentous cotton thread, and the entire system was flushed with a solution of heparin sulphate (100 IU/mL) in normal saline.…”

Section: Methodsmentioning

confidence: 99%

Interstitial fluid pressure in cervical carcinoma

Milosevic

Fyles

Wong

et al. 1998

Cancer

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Section: Methodsmentioning

confidence: 99%

Interstitial fluid pressure in cervical carcinoma

Milosevic

Fyles

Wong

et al. 1998

Cancer

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“…Interstitial fluid pressure was measured in orthotopic and subcutaneous tumours using the wick-in-needle technique (Fadnes et al, 1977). In brief, a hypodermic needle (size G23) was placed centrally in the tumour and connected to a pressure transducer (SensoNor 840, Norway) via polyethylene tubing (PI-50, BectonDickinson, MD, USA) filled with sterile heparinised PBS (70 U ml À1 ).…”

Section: Interstitial Fluid Pressurementioning

confidence: 99%

Hyaluronidase induces a transcapillary pressure gradient and improves the distribution and uptake of liposomal doxorubicin (Caelyx™) in human osteosarcoma xenografts

et al. 2005

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Liposomal drug delivery enhances the tumour selective localisation and may improve the uptake compared to free drug. However, the drug distribution within the tumour tissue may still be heterogeneous. Degradation of the extracellular matrix is assumed to improve the uptake and penetration of drugs. The effect of the ECM-degrading enzyme hyaluronidase on interstitial fluid pressure and microvascular pressure were measured in human osteosarcoma xenografts by the wick-in-needle and micropipette technique, respectively. The tumour uptake and distribution of liposomal doxorubicin were studied on tumour sections by confocal laser scanning microscopy. The drugs were injected i.v. 1 h after the hyaluronidase pretreatment. Intratumoral injection of hyaluronidase reduced interstitial fluid pressure in a nonlinear dose-dependent manner. Maximum interstitial fluid pressure reduction of approximately 50% was found after injection of 1500 U hyaluronidase. Neither intratumoral nor i.v. injection of hyaluronidase induced any changes in the microvascular pressure. Thus, hyaluronidase induced a transcapillary pressure gradient, resulting in a four-fold increase in the tumour uptake and improving the distribution of the liposomal doxorubicin. Hyaluronidase reduces a major barrier for drug delivery by inducing a transcapillary pressure gradient, and administration of hyaluronidase adjuvant with liposomal doxorubicin may thus improve the therapeutic outcome.

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“…Tumour IFP was measured using the wick-in-needle technique (Fadnes et al, 1977). and the transducer using a screw clamp (Tufto and Rofstad, 1995).…”

Section: Measurement Of Interstitial Fluid Pressurementioning

confidence: 99%

Proton relaxation times and interstitial fluid pressure in human melanoma xenografts

Lyng

Tufto²,

Skretting³

et al. 1997

Br J Cancer

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Summary The interstitial fluid pressure (IFP) and the proton spin-lattice and spin-spin relaxation times (T, and T2) of some experimental tumours have been shown to be related to tumour water content. These observations have led to the hypothesis that magnetic resonance imaging (MRI) might be a clinically useful non-invasive method for assessment of tumour IFP. The purpose of the work reported here was to examine the general validity of this hypothesis. R-1 8 human melanoma xenografts grown intradermally in Balb/c nu/nu mice were used as the tumour model system. Median T, and T2 were determined by spin-echo MRI using a 1 .5-T clinical whole-body tomograph. IFP was measured using the wick-in-needle technique. No correlation was found between tumour IFP and fractional tumour water content. Moreover, there was no correlation between median T, or T2 and IFP, suggesting that proton T, and T2 values determined by MRI cannot be used clinically to assess tumour IFP and thereby to predict the uptake of macromolecular therapeutic agents.Keywords: melanoma xenografts; interstitial fluid pressure; magnetic resonance imaging; relaxation times; tumour water content Most tumours show an elevated interstitial fluid pressure (IFP) compared with normal tissues (Jain, 1987). Highly elevated IFP might lead to a pressure difference between the microvascular and interstitial space that is close to 0 mmHg and hence to inadequate uptake and heterogeneous distribution of monoclonal antibodies and other macromolecular therapeutic agents (Jain and Baxter, 1988;Cobb, 1989). Many human tumours show a maximum antibody uptake per gram of tissue of only around 0.005% of the injected dose per gram of body weight (Bradwell et al, 1985). Antibodies are preferentially distributed in regions close to blood vessels, and there are many regions without or with negligible antibody uptake (Jones et al 1986;Sands et al, 1988).Measurements of IFP in human tumours using the wickin-needle technique have shown that the IFP can differ substantially among individual tumours of the same histological type (Boucher et al, 1991;Less et al, 1992). Patients with tumours showing an IFP close to normal tissue values, i.e. tumours with a significant pressure difference between the microvascular and interstitial space are more likely to benefit from treatment modalities involving macromolecular therapeutic agents than patients with highly elevated tumour IFP (Jain and Baxter, 1988). The wickin-needle technique is invasive and can be used to measure IFP only in superficial tumours. A non-invasive method for measurement of IFP would therefore be useful for predicting the uptake of macromolecules in tumours and hence therapeutic response.Studies of experimental tumours have suggested that the IFP is related to tumour water content in some tumour lines (Lee et al, 1992;Leunig et al, 1994). The proton spin-lattice and spin-spin relaxation times (T1 and T2) of tumour tissue are, to a large extent, (Braunschweiger et al, 1986;Belfi et al, 1991). These observations have le...

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Interstitial fluid pressure in rats measured with a modified wick technique

Cited by 177 publications

References 19 publications

Interstitial fluid pressure in cervical carcinoma

Interstitial fluid pressure in cervical carcinoma

Hyaluronidase induces a transcapillary pressure gradient and improves the distribution and uptake of liposomal doxorubicin (Caelyx™) in human osteosarcoma xenografts

Proton relaxation times and interstitial fluid pressure in human melanoma xenografts

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