Interstitial Collagenase Gene Expression Correlates With In Vitro Invasion in Human Chondrosarcoma

Scully, Sean P.; Berend, Keith R.; Toth, Alison P.; Qi, Wen-Ning; Zhou, Qi; Block, Joel A.

doi:10.1097/00003086-200007000-00038

Cited by 54 publications

(54 citation statements)

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“…It has been shown that overexpression of TIMP markedly reduces the invasiveness of tumor cells both in vitro and in vivo. These studies have provided clear functional evidence for the 55012 cells, a poorly differentiated human CS cell line, express relatively large amounts of MMP-1 [27]. These cells have significantly higher collagenase activity and invasion capability than other moderately and highly differentiated CS cells [27].…”

Section: Discussionmentioning

confidence: 87%

“…These studies have provided clear functional evidence for the 55012 cells, a poorly differentiated human CS cell line, express relatively large amounts of MMP-1 [27]. These cells have significantly higher collagenase activity and invasion capability than other moderately and highly differentiated CS cells [27]. This study employs a vector-based siRNA system to generate a CS cell line that constitutively knocked down the expression of MMP-1 .…”

Section: Discussionmentioning

confidence: 99%

“…55012, an established human CS cell line, was cultured as previously described [16,27]. Transfection of siRNAs was performed by using Lipofectmine 2000 siRNA transfection reagent (Invitrogen).…”

Section: Cell Culture and Transient Transfection Of Sirnas Oligosmentioning

confidence: 99%

“…Chondrosarcoma (CS) is the second most common malignancy of bone [2,26,27]. The malignancy demonstrates local invasion, dedifferentiation, and metastasis.…”

Section: Introductionmentioning

confidence: 99%

“…The malignancy demonstrates local invasion, dedifferentiation, and metastasis. We have shown a marked prognostic significance for recurrence in the patients expressing high levels of matrix metalloproteinases-1 (MMP-1) [2,26] and believe that this enzyme facilitates cellular egress from the tumor matrix [27]. One mechanism by which MMP-1 may promote tumor recurrence is by degrading the extracellular matrix (ECM) [ 181.…”

Section: Introductionmentioning

confidence: 99%

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RNAi mediated MMP-1 silencing inhibits human chondrosarcoma invasion

2005

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Previous work indicates that matrix metalloproteinase-1 (MMP-1) gene expression is a prognostic factor for local recurrence and metastasis in human chondrosarcoma. The current study evaluates the effects of MMP-1 knock-down via RNA interference on chondrosarcoma metastasis in vitro. Of nine siRNA oligos tested, one showed a 90% inhibitory effect on MMP-1 gene expression. Stable attenuation of MMP-1 in chondrosarcoma cells was achieved by using a plasmid vector-based siRNA system. Propounced MMP-1 activity suppression was accompanied by marked inhibition of tumor cell invasion. Cells in which MMP-1 has been stably silenced are useful tools to elucidate the mechanism of MMP-1 mediated tumor metastasis.

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Section: Discussionmentioning

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Section: Cell Culture and Transient Transfection Of Sirnas Oligosmentioning

confidence: 99%

“…Chondrosarcoma (CS) is the second most common malignancy of bone [2,26,27]. The malignancy demonstrates local invasion, dedifferentiation, and metastasis.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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RNAi mediated MMP-1 silencing inhibits human chondrosarcoma invasion

2005

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A synthetic triterpenoid selectively inhibits the induction of matrix metalloproteinases 1 and 13 by inflammatory cytokines

Mix¹,

Mengshol²,

Benbow³

et al. 2001

Arthritis & Rheumatism

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Antiproliferative effect of the histone demethylase inhibitor GSK‐J4 in chondrosarcomas

et al. 2019

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Chondrosarcoma (CS) is the second most common malignant bone sarcoma. Its treatment remains an issue, because this tumor is radio-and chemo-resistant. In the present study, we investigated the antitumoral potential of GSK-J4, a small molecule described as an inhibitor of histone demethylases UTX and JMJD3 (KDM6A and KDM6B), alone or in combination with cisplatin in CSs. Human CS-derived cell lines were treated with GSK-J4 in the presence or not of cisplatin. Survival curves were established and cell proliferation and cycle were evaluated by flow cytometry using dividing cell tracking technique utilizing carboxyfluorescein succinimidyl ester labeling, or DNA staining by propidium iodide. Apoptosis and senescence were also investigated. GSK-J4 decreased proliferation of CS cells. Additionally, it induced apoptosis in CH2879 and JJ012 cells, but not in SW1353 CSs. In addition, its association with cisplatin decreased cell proliferation more than drugs alone, whereas it did not increase apoptosis compared to cisplatin alone. Interestingly, GSK-J4 alone as well as in association with cisplatin did not affect chondrocyte survival or proliferation. In conclusion, this study suggests that demethylase inhibitors may be useful in improving therapy for CS in reducing its proliferation. K E Y W O R D Sapoptosis, cell death, chondrosarcoma, histone demethylase, senescence

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Interstitial Collagenase Gene Expression Correlates With In Vitro Invasion in Human Chondrosarcoma

Cited by 54 publications

References 50 publications

RNAi mediated MMP-1 silencing inhibits human chondrosarcoma invasion

RNAi mediated MMP-1 silencing inhibits human chondrosarcoma invasion

A synthetic triterpenoid selectively inhibits the induction of matrix metalloproteinases 1 and 13 by inflammatory cytokines

Antiproliferative effect of the histone demethylase inhibitor GSK‐J4 in chondrosarcomas

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