Interstitial Class Ii-Positive Cell Depletion by Donor Pretreatment With Gamma Irradiation

Stegall, Mark D.; Tezuka, K; Oluwole, Soji F.; Engelstad, Kristin; Jing, Ming X.; Andrew, James E.; Hardy, Mark A.

doi:10.1097/00007890-199002000-00003

Cited by 29 publications

(3 citation statements)

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“…Alternatively, the type of passenger leukocytes may be different in the two types of organ grafts carrying donor-derived cells, as the function or characterization of donor passenger leukocytes may independently and respectively be modulated by the existence of a liver or a heart allo-graft. Although we performed HHI' 24 h after TBI of the donor in the combination DA-to-PVG, others have shown that an increased interval between TBI and transplantation in other combinations appears to prolong allograft survival [6,20,28].…”

Section: Discussionmentioning

confidence: 96%

“…Rat, liver transplantation, passenger leukocytes . Tolerance, liver transplantation, rat creas islet, kidney, heart, and liver transplantation in several rejector combinations 16, 21,22,23,28,301. Donor-derived passenger leukocytes, in particular den-However, &cent studies [26,291 show that the removal dritic cells, provide the trigger for allograft rejection of passenger leukocytes by TBI of the donor appears to since these cells may cause sensitization of host T cells be associated with not only the suppression of rejection, to donor antigens [5,14,151.…”

Section: Introductionmentioning

confidence: 99%

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The characterization of reconstituted passenger leukocytes on the induction of tolerance in rat liver transplantation

et al. 1997

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The tolerance induced by orthotopic liver transplantation [DA (RT1a) rats to PVG (RT1c) rats] can be prevented by total body irradiation of the donor rat. Reconstitution of the irradiated donor with DA splenic leukocytes reintroduces this tolerance. To investigate the major histocompatibility complex (MHC) specificity of passenger leukocytes, irradiated DA donors were reconstituted by third-party BN (RT1n) splenic leukocytes. The reconstitution with BN splenocytes re-established DA-specific tolerance in PVG recipients, as confirmed by subsequent DA cardiac allografting, while BN hearts were rejected with second-set tempo. To determine which cell components play an important role in re-establishing liver graft tolerance, DA splenic leukocytes were further purified into three types: T, B, and adherent cells. Only "T-cell-enriched" preparations restored liver graft tolerance in three out of five PVG recipients. These results suggest that passenger leukocytes of differing MHC types can help to induce liver-specific tolerance and that T cells in the liver graft may be essential to regulate tolerance induction.

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Section: Discussionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

The characterization of reconstituted passenger leukocytes on the induction of tolerance in rat liver transplantation

et al. 1997

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“…Alternatively, the type of passenger leukocytes may be different in the two types of organ grafts carrying donor-derived cells, as the function or characterization of donor passenger leukocytes may independently and respectively be modulated by the existence of a liver or a heart allograft. Although we performed HHI' 24 h after TBI of the donor in the combination DA-to-PVG, others have shown that an increased interval between TBI and transplantation in other combinations appears to prolong allograft survival [6,20,28].…”

Section: Discussionmentioning

confidence: 96%

The characterization of reconstituted passenger leukocytes on the induction of tolerance in rat liver transplantation

Chiba

Goto

Shimizu

et al. 1997

Transplant International

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Intestinal allograft survival in the rat following pretreatment with donor-specific UV-B-irradiated leukocytes and peritransplant immunosuppression with cyclosporine

et al. 1992

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Previous studies from our laboratory showed that pretreatment with ultraviolet-B-irradiated donor leukocytes (UV-B DL) combined with brief peritransplant cyclosporine (CyA) resulted in indefinite survival of Wistar/Furth rat cardiac allografts in Lewis recipients. This study was designed to examine the effect of pretransplant UV-B DL with or without peritransplant CyA on orthotopic intestinal allografts in the same rat strain combination. The results showed that while low-dose CyA treatment alone (10 mg/kg i.m. on days 0, +1, and +2) had no effect on intestinal allograft rejection, 20 mg/kg (on days 0, +1, and +2) CyA significantly (P less than or equal to 0.001) prolonged graft survival, with 33% of the hosts surviving indefinitely. The highest dose of CyA (30 mg on days 0, +1, and +2) abrogated rejection, but most transplant recipients succumbed to infection and functional ileus due to a toxic side effect of CyA. Pretreatment with UV-B DL on days -14 and -7 alone did not prolong intestinal allograft survival. Combination of a subtherapeutic CyA dose (20 or 10 mg/kg) given on days 0, +1, and +2 with pretransplant UV-B DL on days -14 and -7 did not alter the survival of intestinal allografts compared to treatment with CyA alone. This suggests that pretreatment with UV-B DL with or without peritransplant administration of CyA has no effect on intestinal allograft survival, in contrast to the effect of such combined treatment on cardiac allograft survival, where indefinite graft survival is observed.(ABSTRACT TRUNCATED AT 250 WORDS)

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Interstitial Class Ii-Positive Cell Depletion by Donor Pretreatment With Gamma Irradiation

Cited by 29 publications

References 0 publications

The characterization of reconstituted passenger leukocytes on the induction of tolerance in rat liver transplantation

The characterization of reconstituted passenger leukocytes on the induction of tolerance in rat liver transplantation

The characterization of reconstituted passenger leukocytes on the induction of tolerance in rat liver transplantation

Intestinal allograft survival in the rat following pretreatment with donor-specific UV-B-irradiated leukocytes and peritransplant immunosuppression with cyclosporine

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