Interstitial cells of Cajal are normally distributed in both ganglionated and aganglionic bowel in Hirschsprung?s disease

Newman, Christopher J.; Laurini, Ricardo; Lesbros, Y; Reinberg, Olivier; Meyrat, Blaise

doi:10.1007/s00383-003-1026-1

Cited by 34 publications

(21 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Data from these animals suggest that the presence of neurons is not required for the formation of a mature ICC network and that the circular muscle layer, which develops before the ICC, can provide sufficient SCF for the formation of functional ICC networks. However, the developmental relationship between neurons and ICC is still contentious, particularly in humans, in which conflicting findings have recently been described (Huizinga et al 2001;Rolle et al 2002;Newman et al 2003).…”

Section: Discussionmentioning

confidence: 96%

Development of the enteric nervous system, smooth muscle and interstitial cells of Cajal in the human gastrointestinal tract

2005

View full text Add to dashboard Cite

The generation of functional neuromuscular activity within the pre-natal gastrointestinal tract requires the coordinated development of enteric neurons and glial cells, concentric layers of smooth muscle and interstitial cells of Cajal (ICC). We investigated the genesis of these different cell types in human embryonic and fetal gut material ranging from weeks 4-14. Neural crest cells (NCC), labelled with antibodies against the neurotrophin receptor p75NTR, entered the foregut at week 4, and migrated rostrocaudally to reach the terminal hindgut by week 7. Initially, these cells were loosely distributed throughout the gut mesenchyme but later coalesced to form ganglia along a rostrocaudal gradient of maturation; the myenteric plexus developed primarily in the foregut, then in the midgut, and finally in the hindgut. The submucosal plexus formed approximately 2-3 weeks after the myenteric plexus, arising from cells that migrated centripetally through the circular muscle layer from the myenteric region. Smooth muscle differentiation, as evidenced by the expression of alpha-smooth muscle actin, followed NCC colonization of the gut within a few weeks. Gut smooth muscle also matured in a rostrocaudal direction, with a large band of alpha-smooth muscle actin being present in the oesophagus at week 8 and in the hindgut by week 11. Circular muscle developed prior to longitudinal muscle in the intestine and colon. ICC emerged from the developing gut mesenchyme at week 9 to surround and closely appose the myenteric ganglia by week 11. By week 14, the intestine was invested with neural cells, longitudinal, circular and muscularis mucosae muscle layers, and an ICC network, giving the fetal gut a mature appearance.

show abstract

Section: Discussionmentioning

confidence: 96%

Development of the enteric nervous system, smooth muscle and interstitial cells of Cajal in the human gastrointestinal tract

2005

View full text Add to dashboard Cite

show abstract

“…Furthermore, in the oesophagus and stomach cardia, c-kit-IR cells first appear in the MP region (Radenkovic et al 2009), around the ganglia, and, in later developmental stages, the number is markedly reduced or completely disappears in the region. Whether ICC differentiation requires neural crest cells has not been clearly established, although some recent studies have identified ICC in the absence of neural crest cells (Wu et al 2000;Newman et al 2003).…”

Section: Discussionmentioning

confidence: 98%

“…By week 14, the ICC form a network surrounding the MP (Fu et al 2004;Wallace and Burns 2005). Whether ICC differentiation requires neural crest cells has not been clearly established, although some recent studies have identified ICC in the absence of neural crest cells (Newman et al 2003).…”

Section: Introductionmentioning

confidence: 99%

C-kit-immunopositive interstitial cells of Cajal in human embryonal and fetal oesophagus

Radenković

Ilić

Živanović³

et al. 2010

Cell Tissue Res

View full text Add to dashboard Cite

Interstitial cells of Cajal (ICC) are morphologically and functionally intercalated between the elements of the enteric nervous system and the smooth muscle cells (SMCs) in the musculature of the digestive tract. Kit immunohistochemistry reliably identifies the location of these cells and provides information on changes in ICC distribution and density. Human oesophagus specimens (7 embryos, 23 fetuses at 7-27 weeks gestational age; both sexes) were exposed to Kit antibodies to determine ICC differentiation. Enteric plexuses were examined immunohistochemically by using anti-neuron-specific enolase, whereas the differentiation of SMCs was studied with antibodies against alpha-smooth-muscle actin and desmin. By week 7, c-kit-immunopositive cells were present along the entire oesophagus in the form of an uninterrupted layer around the myenteric plexus (MP) elements. From the beginning of the 3rd month, the number of ICC progressively decreased around the MP ganglia but increased within the muscle layers. Concomitantly, differences in the number and distribution of ICC were established in the various portions of the oesophagus: specifically, ICC were abundant in the lower portion, less numerous in the middle region and rare in the upper part. By the 5th month of development, the relationship as found in later developmental stages had been established: C-kit IR ICC were present within the circular muscle layer, within the longitudinal layer and in the connective septa surrounding the muscle bundles but were completely missing around the MP ganglia.

show abstract

“…This study did not differentiate between myenteric and muscular ICCs. Two further, recently published studies showed that ICCs were either normal or only slightly reduced in the affected bowel in HD compared to normal bowel [105,106].…”

Section: Hirschsprung's Diseasementioning

confidence: 95%

Interstitial cells of Cajal in the normal gut and in intestinal motility disorders of childhood

2007

View full text Add to dashboard Cite

Interstitial cells of Cajal (ICCs) are pacemaker cells which are densely distributed throughout the whole gastrointestinal tract. ICCs have important functions in neurotransmission, generation of slow waves and regulation of mechanical activities in the gastrointestinal tract, especially for the coordinated gastrointestinal peristalsis. Therefore, a loss of ICCs could result in gastrointestinal motor dysfunction. In recent years c-kit labeling has been widely used to study pathological changes of ICCs in gastrointestinal motility disorders. Paediatric gastrointestinal motility disorders such as hypertrophic pyloric stenosis, Hirschsprung's disease, total colonic aganglionosis, hypoganglionosis, intestinal neuronal dysplasia, internal anal sphincter achalasia, megacystis microcolon intestinal hypoperistalsis syndrome have been reported to be associated with loss or deficiency of ICCs networks. This review describes the distribution of ICCs in the normal gastrointestinal tract and its altered distribution in intestinal motility disorders of childhood.

show abstract

Interstitial cells of Cajal are normally distributed in both ganglionated and aganglionic bowel in Hirschsprung?s disease

Cited by 34 publications

References 32 publications

Development of the enteric nervous system, smooth muscle and interstitial cells of Cajal in the human gastrointestinal tract

Development of the enteric nervous system, smooth muscle and interstitial cells of Cajal in the human gastrointestinal tract

C-kit-immunopositive interstitial cells of Cajal in human embryonal and fetal oesophagus

Interstitial cells of Cajal in the normal gut and in intestinal motility disorders of childhood

Contact Info

Product

Resources

About