Interstitial and free lung cells in acute inflammation in the guinea-pig

Snella, Marie-Claire; Venaille, T.; Holt, Patrick G.; Rylander, Ragnar

doi:10.1007/bf02009055

Cited by 15 publications

(11 citation statements)

References 22 publications

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“…These guinea-pigs invariably developed a pronounced pulmonary airway eosinophilia which persisted for at least one week. Preliminary histological studies indicated that there was a good correlation between bronchial tissue eosinophilia and eosinophils collected in BAL fluid, in agreement with earlier studies (Snella et al, 1987;Dunn et al, 1988). Accordingly, we have chosen to assess eosinophil accumulation in BAL fluid rather than by histology, due to the difficulty in evaluating histological specimens of lung tissue from large numbers of guineapigs.…”

Section: Discussionsupporting

confidence: 77%

Antigen challenge induces pulmonary airway eosinophil accumulation and airway hyperreactivity in sensitized guinea‐pigs: the effect of anti‐asthma drugs

Sanjar

Kristersson

et al. 1990

British J Pharmacology

188

114

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Guinea‐pigs were sensitized with 3 injections of ovalbumin (OA) (1 or 10 μg per animal) using Al(OH)3 and pertussis vaccine as adjuvants at two week intervals. Sensitized guinea‐pigs were challenged with an aerosol of OA (0.1%) over a one hour period and both airway reactivity and cellular content of bronchoalveolar lavage (BAL) fluid were assessed at intervals for up to 7 days. Guinea‐pigs sensitized with 1 μg of ovalbumin responded to an aerosol of OA with increased pulmonary airway eosinophilia, which was evident 1 day after challenge and was present for up to 7 days. Airway hyperreactivity was not detectable in these animals. Guinea‐pigs sensitized with 10 μg of ovalbumin responded to an aerosol of OA with increased pulmonary airway neutrophilia and eosinophilia and with increased airway reactivity which was maximal between 8 and 24 h after exposure to OA. Depletion of circulating platelets or neutrophils, by use of selective antisera, did not alter either the magnitude of eosinophilia or the intensity of airway reactivity in sensitized guinea‐pigs (10 μg) exposed to an aerosol of OA. Pretreatment of sensitized guinea‐pigs (10 μg) for 6 days with AH 21–132, aminophylline, dexamethasone or ketotifen inhibited pulmonary airway eosinophilia, but did not diminish airway hyperreactivity. Neither eosinophil accumulation nor development of airway hyperreactivity was influenced by treatment with mepyramine or salbutamol over a 6 day period before OA inhalation. Although eosinophilia may occur in association with increased airway reactivity in this animal model, there is no evidence of a causal relationship.

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Section: Discussionsupporting

confidence: 77%

Antigen challenge induces pulmonary airway eosinophil accumulation and airway hyperreactivity in sensitized guinea‐pigs: the effect of anti‐asthma drugs

Sanjar

Kristersson

et al. 1990

British J Pharmacology

188

114

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“…The effect of endotoxin exposure on cell numbers and particularly lung lavage neutrophils, confirms observa tions in previous studies [5,9,11].…”

Section: Discussionsupporting

confidence: 78%

“…These cells were prepared according to a tech nique reported earlier [11]. Animals were sacrificed and the aorta was severed above the liver.…”

Section: Cell Preparationsmentioning

confidence: 99%

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Oxygen Metabolites from Lavage and Interstitial Lung Cells after Inhalation of Endotoxin in Guinea Pigs

Hsieh

Amoruso-Marchat²,

Rylander³

et al. 1994

Int Arch Allergy Immunol

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Airborne endotoxins play a role in a variety of occupational diseases such as byssinosis and humidifier’s disease and are associated with pulmonary and systemic symptoms. An excess generation of oxygen free radicals (including superoxide anion, O^––₂) by inflammatory cells has been suggested in endotoxemia. We have studied the release of superoxide from guinea-pig lung lavage and interstitial cells and blood monocytes (BMs) at different times after an acute inhalation exposure to bacterial endotoxin. O^––₂ generation was measured by the O^––₂ dismutase-inhibitable reduction of ferricytochrome c, after stimulation with phorbol-myristate-acetate (PMA) or opsonized zymosan (OZ). After endotoxin exposure the spontaneous release of O^––₂ remained unchanged for the three cell types. From 4 h after exposure until 48 h afterwards, lung lavage cells produced more O^––₂ after PMA or OZ stimulation than did cells from unexposed guinea pigs. The pattern of O^––₂ generation by interstitial cells followed that of lung lavage macrophages. O^––₂ production remained unchanged in BMs at all times. These results suggest that endotoxin inhalation induced a priming of lavage and interstitial cells, mainly macrophages, associated with an increase in O^––₂ production. As O^––₂ production by BMs remained unchanged, this cell is unlikely to be responsible for the systemic symptoms seen after endotoxin inhalation.

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“…It may be argued that the analysis of BAL fluid which consists of surface washings from bronchioles and alveoli does not necessarily reflect events within the respiratory tissue itself. On the other hand, Snella et al (1987) have shown a good correlation between bronchial tissue eosinophilia and eosinophils collected in BAL fluid but whether the same is true for other leukocytes is currently unknown. In addition it may not simply be the number of inflammatory cells re-cruited to the lungs, but the activation state of these cells that may be relevant to the development of bronchial hyperreactivity in our model.…”

Section: Discussionmentioning

confidence: 99%

Inhibition by rapamycin of leukocyte migration and bronchial hyperreactivity induced by injection of Sephadex beads to guinea‐pigs

Francischi

Conroy

Maghni

et al. 1993

British J Pharmacology

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1 The effect of rapamycin (0.001 to 5 mg kg-') on the increased leukocyte counts in bronchoalveolar lavage (BAL) fluid and hyperreactivity of isolated bronchial strips to histamine and acetylcholine (ACh) was studied following the intravenous injection of Sephadex beads to guinea-pigs.2 The intramuscular (i.m.) injection of rapamycin (0.012 to 5 mg kg-') dose-dependently inhibited the increase in leukocyte counts in BAL fluid. Rapamycin (5 mg kg-1) reduced the numbers of eosinophils neutrophils, macrophages and lymphocytes in BAL fluid by 64, 55, 19 and 50% respectively. In addition, rapamycin (0.0 12 to 5 mg kg-') significantly inhibited the Sephadex-induced hyperreactivity of bronchial tissue to both histamine and ACh.3 At a dose of 0.001 mg kg-', rapamycin did not significantly reduce leukocyte infiltration or bronchial hyperreactivity. 4 Cyclosporin (5 mg kg-') significantly reduced both lymphocyte and eosinophil numbers in BAL fluid of Sephadex-injected guinea-pigs whereas dexamethasone (1 mg kg-') significantly reduced lymphocyte numbers. Neither drug affected the bronchial hyperreactivity to histamine and ACh. 5 It is concluded that the new immunosuppressive drug, rapamycin, is a potent inhibitor of leukocyte migration and bronchial hyperreactivity observed following the intravenous injection of Sephadex beads to guinea-pigs. Rapamycin also appears to be more effective than cyclosporin or dexamethasone in reducing leukocyte counts and bronchial hyperreactivity in this model. 6 Our results suggest that inflammatory mechanisms which are inhibited by rapamycin may be important in the induction of Sephadex-induced hyperreactivity.

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Interstitial and free lung cells in acute inflammation in the guinea-pig

Cited by 15 publications

References 22 publications

Antigen challenge induces pulmonary airway eosinophil accumulation and airway hyperreactivity in sensitized guinea‐pigs: the effect of anti‐asthma drugs

Antigen challenge induces pulmonary airway eosinophil accumulation and airway hyperreactivity in sensitized guinea‐pigs: the effect of anti‐asthma drugs

Oxygen Metabolites from Lavage and Interstitial Lung Cells after Inhalation of Endotoxin in Guinea Pigs

Inhibition by rapamycin of leukocyte migration and bronchial hyperreactivity induced by injection of Sephadex beads to guinea‐pigs

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