Interspecific hybridization of Cucumis anguria and C. zeyheri via embryo-rescue

Abstract

Embryo-rescue was used to facilitate interspecific hybridization of Cucumis anguria L. and C. zeyheri Sond. Embryos were excised from developing fruits at one week intervals for six weeks after hand pollination. Medium containing coconut water was the most suitable for initial germination, and a medium with ascorbic acid was the best for embryo development and plant recovery. Viable plants were obtained from embryos and these plants showed morphological characteristics different from both parents. The analysis of the leucine aminopeptidase (LAP) locus revealed three hybrid types, H1.1, H1.2 and H2.

Response of Wild Cucumis Species to Inoculation with Fusarium oxysporum f. sp. melonis Race 1,2y

“…Because some wild Cucumis species have high resistance to race 1,2y, it would be valuable to study the use of wild Cucumis species as genetic resources of high resistance to race 1,2y. To date, few interspecific hybrids between melon and wild Cucumis species have been reported because of strong reproductive barriers (Beherav and Choen, 1995;Deakin et al, 1971;Fassuliotis, 1977;Kho et al, 1980;Fujishita, 1969, 1970;Singh and Yadava, 1984); however, some interspecific hybrids of genus Cucumis were obtained through embryo culture (Chen et al, 1997;Fassuliotis and Nelson, 1988;Skálová et al, 2008). To introduce this high resistance to race 1,2y into melon cultivars, methods to overcome reproductive barriers in interspecific crosses between melon and wild Cucumis species, such as embryo cultures, should be investigated.…”

Development of novel chloroplast microsatellite markers for Cucumis from sequence database

“…However, there is a lack of cpSSR markers capable of detection of high-level polymorphism in Cucumis and the other closely related genera. In addition, development of cpSSR markers and analysis of Cucumis plastome polymorphism allow a more efficient search for cytoplasm donors that can be used to breed new cultivars, especially for cucumber that has a narrow genetic base (Skálová et al 2008). In present study, we developed a set of cpSSR markers for Cucumis and examined their transferability on the other Cucurbitaceae species.…”

Biotechnological methods utilized in Cucumis research - A review

“…Nevertheless one case of interspecific hybridization was successful; hybrid plants were obtained after cross pollination of C. anguria with C. zeyheri. The hybrids were confirmed by isozyme and morphological analyses (Skálová et al 2008c).…”