Interspecific hybridization by sexual means

Pickersgill, Barbara

doi:10.1007/978-94-011-1524-7_6

Cited by 18 publications

(12 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This outcome is in contrast with the results of Ahmad et al (1995) who obtained viable lentil hybrids between cultivars of New Zealand crossed with L. odemensis, L. ervoides and L. nigricans applying gibberellic acid after pollination. Nevertheless, it has been well described that certain cultivars and accessions of a species are better combiners to recover interspecific hybrids than other cross combinations (Pitarelli and Stavely, 1975;Harlan and de Wet, 1977;Williams, 1987;Pickersgill, 1993).…”

Section: Resultsmentioning

confidence: 99%

“…These dissimilarities could be due to the fact that different ovule ages (14 vs. 18 DAP, respectively) and culture media were used. It is a well known fact, that with younger ovule-embryos the difficulty of rescue increases, together with their sensibility to phytohormone and carbohydrate concentrations of the media (Williams, 1987;Pickersgill, 1993).…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Interspecific Hybridization in the Genus Lens Applying in Vitro Embryo Rescue

Fratini

Ruiz

2006

Euphytica

View full text Add to dashboard Cite

Four different hybridization experiments were carried out to obtain interspecific hybrids with Spanish cultivars of lentil (Lens culinaris M.). In hybridization experiments I and II, undertaken with only pollination and pollination with the addition of gibberellic acid after fertilization, respectively, no lentil hybrids were recovered. A single interspecific hybrid with L. odemensis was obtained in experiment III using the embryo rescue protocol of Cohen et al. (1984), in this case, a crossing efficiency of 0.11% and a rescue efficiency of 2.5% were obtained. Hybridization experiment IV used a specific embryo rescue protocol developed in this study. In this experiment, ovule-embryos of 18 DAP were cultured on MS salts with 1% sucrose and 1 μM IAA + 0.8 μM KN; after two weeks, embryos were released from the ovular integuments and cultured on the same medium for another two weeks in upright position. Afterwards, the embryos were transferred to test tubes containing the same medium and one month later plantlets were obtained. Using the above protocol, out of a total of 1707 pollinations, 6 interspecific hybrids with L. odemensis, 2 with L. nigricans and one with L. ervoides were recovered, yielding on average a crossing efficiency of 0.53% and an average rescue efficiency of 8.26%. Taking into consideration only the interspecific crossing blocks in which hybrids were recovered, the crossing efficiency with L. odemensis was 9%, while with both L. nigircans and L. ervoides the crossing efficiencies were 3%. Rescue efficiencies based on hybrids recovered per number of ovules cultured ranged between 50-100%.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Interspecific Hybridization in the Genus Lens Applying in Vitro Embryo Rescue

Fratini

Ruiz

2006

Euphytica

View full text Add to dashboard Cite

show abstract

“…Differences in ploidy level are often an important incongruity barrier in interspecific hybridization resulting in malformation of endosperm and the inhibition of germination (Badger 1988). Endosperm absence or retarded development often causes spontaneous abortion of the fruits (Pickersgill et al 1993;Sharma 1995;Van Tuyl et al 1991). Polyploidization offers sometimes a solution towards the problem of parent species having a different ploidy level (Stebbins 1971).…”

Section: Discussionmentioning

confidence: 99%

Interspecific hybridisation and genome size analysis in Buddleja

Laere¹,

Leus²,

Huylenbroeck³

et al. 2008

Euphytica

View full text Add to dashboard Cite

Interspecific hybrids Buddleja davidii x Buddleja weyeriana, Buddleja weyeriana x Buddleja davidii and Buddleja davidii x Buddleja lindleyana were generated using in vitro embryo rescue 10-11 weeks after manual pollination. The morphological variation within the F1 populations was limited. The F1 progeny of B. davidii x B. lindleyana was almost sterile and no F2 generation was obtained. From the other hybrids, F2 generations were made by self pollinations and back crosses. Hybrid nature of all F1 and F2 seedlings was confirmed by AFLP. Chromosome counting and genome size measurement for B. weyeriana (F2 selection of (diploid) B. globosa x (tetraploid) B. davidii) revealed a higher chromosome number (76 chromosomes) and genome size than expected, indicating 2n-gametes formation occurred during meiosis of B. globosa. The F1 hybrids B. weyeriana x B. davidii (76 chromosomes) had an intermediate genome size compared with the genome size of the parent plants, proving their hybrid nature. However, the F1 and F2 hybrids of B. davidii x B. weyeriana all had 76 chromosomes but had a lower genome size than expected, suggesting the occurrence of chromosome rearrangements in the genome of the hybrids. B. lindleyana had 38 chromosomes, while the F1 hybrids of B. davidii x B. lindleyana had 76 chromosomes. Also genome size measurements revealed that the F1 seedlings B. davidii x B. lindleyana had higher genome sizes than expected. Both the results of chromosome counting and genome size measurement indicate that 2n-gametes formation took place during meiosis of B. lindleyana

show abstract

“…Differences in ploidy level can be an incongruity barrier in interspecific hybridisation resulting in malformation of endosperm, the inhibition of germination (Badger 1988), or spontaneous abortion of the fruits (Van Tuyl et al 1991;Pickersgill et al 1993, Sharma 1995. In this study, 182 crosses were made between B. davidii cv.…”

Section: Interspecific Breedingmentioning

confidence: 99%

Introgression of yellow flower colour in Buddleja davidii by means of polyploidisation and interspecific hybridisation

Laere¹,

Huylenbroeck²,

Bockstaele³

2011

Hort. Sci. (Prague)

View full text Add to dashboard Cite

To introduce yellow colour in the commercial Buddleja davidii (2n = 4x = 76) assortment, an interspecific breeding programme with B. globosa (2n = 2x = 38) was started. The first step was to perform chromosome doubling in B. globosa. Two of the obtained tetraploid B. globosa plants were subsequently used as male parent in interspecific crosses with the white flowering B. davidii cv. Nanhoensis Alba. In total 182 interspecific crosses were made and 18 F1 hybrids were obtained. Genome size measurements, chromosome counts and genomic in situ hybridisation (GISH) analysis proved the hybrid nature of most of the F1 hybrids. Plant morphology also expressed hybrid characteristics. F1 seedlings inherited the yellowish flower colour from B. globosa. As for many other woody ornamentals, the creation of hybrids through interspecific hybridisation along with polyploidisation offers new opportunities for breeding in Buddleja.

show abstract

Interspecific hybridization by sexual means

Cited by 18 publications

References 19 publications

Interspecific Hybridization in the Genus Lens Applying in Vitro Embryo Rescue

Interspecific Hybridization in the Genus Lens Applying in Vitro Embryo Rescue

Interspecific hybridisation and genome size analysis in Buddleja

Introgression of yellow flower colour in Buddleja davidii by means of polyploidisation and interspecific hybridisation

Contact Info

Product

Resources

About