Interspecific differences between the DNA restriction profiles of canine adenoviruses

Abstract

Identification of canine adenovirus-1 ( CAV -1) and canine adenovirus-2 ( CAV -2) strains was done by electrophoresis of restriction endonuclease-fragmented viral DNA. Results obtained with this sensitive and reproducible technique clearly show that CAV -2 is not a variant of CAV -1 but a distinct virus.

Canine Adenoviruses and Herpesvirus

“…Because CAV-2 can also be detected in the internal organs and feces of vaccinated or acutely infected dogs [46] and CAV-1 is also frequently isolated from respiratory secretions, trachea, and lungs, distinction between CAV-1 and CAV-2 necessarily deserves laboratory examination. Restriction fragment length polymorphism analysis on viral genomes using the endonucleases PstI and HpaII generates differential patterns [17,18]. Detection and differentiation of CAV-1 and CAV-2 by PCR with a single primer pair are also possible [69].…”

Canine respiratory viruses

“…The isolate, strain Toronto A26/61, was characterized as an adenovirus, and was initially considered to be an attenuated strain of canine adenovirus type 1 (CAV-1). Subsequently, structural and antigenic differences were observed and strain A26/61 was proposed as the prototype of a distinct canine adenovirus, designated as type 2 (CAV-2) [65,75,104,107,152,172]. CAV-1 and CAV-2 were found to be genetically different by restriction endonuclease analysis [10,75] and by DNA hybridization [106].…”

“…Subsequently, structural and antigenic differences were observed and strain A26/61 was proposed as the prototype of a distinct canine adenovirus, designated as type 2 (CAV-2) [65,75,104,107,152,172]. CAV-1 and CAV-2 were found to be genetically different by restriction endonuclease analysis [10,75] and by DNA hybridization [106]. The complete sequence analysis of both the CAV-1 and CAV-2 genome has revealed about 75% nucleotide identity [49,114].…”

A mutant of canine adenovirus type 2 with a duplication of the E1a region exhibits altered expression of early region 4

“…Canine adenovirus type 2 (CAV-2) is a typical member of the Adenoviridae , and has been associated with respiratory and possibly enteric disease in the dog (Ditchfield et al, 1962;Hamelin et al, 1984Hamelin et al, , 1986Macartney et al, 1988). Laboratory attenuated strains of the virus are routinely administered to young dogs as a live vaccine against infectious canine hepatitis and respiratory disease (Koptopoulos & Cornwell, 1981).…”