Interspecies spread of CTX-M-32 extended-spectrum β-lactamase and the role of the insertion sequence IS1 in down-regulating blaCTX-M gene expression

Fernández, Ana; Gil, Emilia; Cartelle, Mónica; Pérez, Astrid; Beceiro, Alejandro; Mallo, Susana; Tomás, María; Pérez-Llarena, Francisco José; Villanueva, Rosa; Bou, Germán

doi:10.1093/jac/dkm030

Cited by 29 publications

(22 citation statements)

References 30 publications

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“…The organization of IS26 and the end of ISEcp1 has been found mostly in clinical Enterobacteriaceae isolates but was also described for an E. coli bla CTX-M-1 -producing strain isolated from fecal droppings of seagulls (12,27,31). On the other hand, the organization of IS5 and the end of ISEcp1 was found upstream of the bla CTX-M-32 gene in environmental and clinical E. coli isolates (13,27). The presence of an ISEcp1 element upstream of bla CTX-M-1 , bla CTX-M-3 , bla CTX-M-14 , and bla CTX-M-15 in clinical isolates was also reported previously (12,20,31).…”

Section: Discussionmentioning

confidence: 87%

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Resistance to Broad-Spectrum Antibiotics in Aquatic Systems: Anthropogenic Activities Modulate the Dissemination of bla _CTX-M -Like Genes

Tacão

Correia

Henriques

2012

Appl Environ Microbiol

137

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We compared the resistomes within polluted and unpolluted rivers, focusing on extended-spectrum beta-lactamase (ESBL) genes, in particular bla CTX-M . Twelve rivers from a Portuguese hydrographic basin were sampled. Physicochemical and microbiological parameters of water quality were determined, and the results showed that 9 rivers were classified as unpolluted (UP) and that 3 were classified as polluted (P). Of the 225 cefotaxime-resistant strains isolated, 39 were identified as ESBL-producing strains, with 18 carrying a bla CTX-M gene (15 from P and 3 from UP rivers). Analysis of CTX-M nucleotide sequences showed that 17 isolates produced CTX-M from group 1 (CTX-M-1, -3, -15, and -32) and 1 CTX-M that belonged to group 9 (CTX-M-14). A genetic environment study revealed the presence of different genetic elements previously described for clinical strains. ISEcp1 was found in the upstream regions of all isolates examined. Culture-independent bla CTX-M -like libraries were comprised of 16 CTX-M gene variants, with 14 types in the P library and 4 types in UP library, varying from 68% to 99% similarity between them. Besides the much lower level of diversity among CTX-M-like genes from UP sites, the majority were similar to chromosomal ESBLs such as bla RAHN-1 . The results demonstrate that the occurrence and diversity of bla CTX-M genes are clearly different between polluted and unpolluted lotic ecosystems; these findings favor the hypothesis that natural environments are reservoirs of resistant bacteria and resistance genes, where anthropogenic-driven selective pressures may be contributing to the persistence and dissemination of genes usually relevant in clinical environments.

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Section: Discussionmentioning

confidence: 87%

“…Sequencing was done for the bla CTX-M gene fragments amplified from the bacterial isolates. The presence of ISEcp1, IS26, IS5, orf477, IS903, and orf503 in the genetic environment of bla CTX-M was searched for by PCR (12,13,31).…”

Section: Methodsmentioning

confidence: 99%

Resistance to Broad-Spectrum Antibiotics in Aquatic Systems: Anthropogenic Activities Modulate the Dissemination of bla _CTX-M -Like Genes

Tacão

Correia

Henriques

2012

Appl Environ Microbiol

137

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“…Upon insertion of the IS, this promoter can activate downstream host genes. Furthermore, insertion of an IS can change the topology of the DNA into which it is inserted and can sometimes introduce or disrupt a regulatory binding sequence, affecting the regulation of the downstream gene(s) (20)(21)(22)(23)(24). Interestingly, the transposition of some ISs can be regulated by certain natural conditions required to activate the transcription of otherwise silent operons (25).…”

Section: Mobile Elementsmentioning

confidence: 99%

Bacterial Genome Instability

Darmon

Leach

2014

Microbiol Mol Biol Rev

335

289

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SUMMARY Bacterial genomes are remarkably stable from one generation to the next but are plastic on an evolutionary time scale, substantially shaped by horizontal gene transfer, genome rearrangement, and the activities of mobile DNA elements. This implies the existence of a delicate balance between the maintenance of genome stability and the tolerance of genome instability. In this review, we describe the specialized genetic elements and the endogenous processes that contribute to genome instability. We then discuss the consequences of genome instability at the physiological level, where cells have harnessed instability to mediate phase and antigenic variation, and at the evolutionary level, where horizontal gene transfer has played an important role. Indeed, this ability to share DNA sequences has played a major part in the evolution of life on Earth. The evolutionary plasticity of bacterial genomes, coupled with the vast numbers of bacteria on the planet, substantially limits our ability to control disease.

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“…In terms of evolution, CTX-M-3 was likely the enzyme at the origin of diversification in the CTX-M-1 cluster, which was coincident with positive selection acting on several amino acid positions [6,15]. Analysis of sequencing, showing that CTX-M-32 differs from CTXM-1 by a single Asp240-Gly substitution, is a key factor in the evolution of CTX-M beta-lactamases for its increased level of resistance to ceftazidime.…”

Section: Resultsmentioning

confidence: 99%

CTX-M-32 beta-lactamase-producing uropathogenic Escherichia coli isolated in Latin America

Araque

Millán

Mendoza³

et al. 2013

J Infect Dev Ctries

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Interspecies spread of CTX-M-32 extended-spectrum β-lactamase and the role of the insertion sequence IS1 in down-regulating blaCTX-M gene expression

Cited by 29 publications

References 30 publications

Resistance to Broad-Spectrum Antibiotics in Aquatic Systems: Anthropogenic Activities Modulate the Dissemination of bla _CTX-M -Like Genes

Resistance to Broad-Spectrum Antibiotics in Aquatic Systems: Anthropogenic Activities Modulate the Dissemination of bla _CTX-M -Like Genes

Bacterial Genome Instability

CTX-M-32 beta-lactamase-producing uropathogenic Escherichia coli isolated in Latin America

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Interspecies spread of CTX-M-32 extended-spectrum β-lactamase and the role of the insertion sequence IS1 in down-regulating blaCTX-M gene expression

Cited by 29 publications

References 30 publications

Resistance to Broad-Spectrum Antibiotics in Aquatic Systems: Anthropogenic Activities Modulate the Dissemination of bla CTX-M -Like Genes

Resistance to Broad-Spectrum Antibiotics in Aquatic Systems: Anthropogenic Activities Modulate the Dissemination of bla CTX-M -Like Genes

Bacterial Genome Instability

CTX-M-32 beta-lactamase-producing uropathogenic Escherichia coli isolated in Latin America

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Product

Resources

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Resistance to Broad-Spectrum Antibiotics in Aquatic Systems: Anthropogenic Activities Modulate the Dissemination of bla _CTX-M -Like Genes

Resistance to Broad-Spectrum Antibiotics in Aquatic Systems: Anthropogenic Activities Modulate the Dissemination of bla _CTX-M -Like Genes