Interspecies somatic cell nuclear transfer in Asiatic cheetah using nuclei derived from post-mortem frozen tissue in absence of cryo-protectant and in vitro matured domestic cat oocytes

Moulavi, F.; Hosseini, Seyed Morteza; Tanhaie-Vash, N.; Ostadhosseini, S.; Hosseini, Seyed Hossein; Hajinasrollah, Mostafa; Asghari, Matin; Gourabi, Hamid; Shahverdi, Abdolhossein; Dizaj, Ahmad Vosough; Nasr-Esfahani, Mohammad Hossein

doi:10.1016/j.theriogenology.2016.11.023

Cited by 23 publications

(16 citation statements)

References 42 publications

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“…In wild felids, somatic cells can be obtained by biopsies (Verma, Holland, Temple‐Smitha, & Verma, ) or necropsy (Moulavi et al, ) from various tissues, including skin from the ear (Praxedes et al, ), inner groin area (Mestre‐Citrinovitz, Sestelo, Ceballos, Barañao, & Saragueta, ), and abdomen (Wittayarat et al, ), as well as muscle (Thongphakdee et al, ), oral mucosa, bone marrow, spinal cord, and intestines (León‐Quinto et al, ) of both genders (Thongphakdee et al, ; Wittayarat et al, ) and different ages (Hashem, Bhandari, Kang, & Lee, ). In general, most studies have used skin tissues, especially from the ear, due to its ease in acquisition during the routine management of animals (Guan et al, ; Liu, Guo, Liu, Guan, & Ma, ).…”

Section: Sources Of Somatic Tissuesmentioning

confidence: 99%

“…In jaguars ( Panthera onca ), dexmedetomidine hydrochloride is a good sedative (Praxedes et al, ). The region to be biopsied is sanitized and tissue samples are harvested using scalpel blades (Moulavi et al, ). After collection, tissue fragments are transported in phosphate‐buffered saline (PBS) (Veraguas, Gallegos, Castro, & Rodriguez‐Alvarez, ) or Dulbecco Modified Eagle Medium (DMEM) (Moulavi et al, ) containing antibacterial and antifungal agents (Wittayarat et al, ).…”

Section: Sources Of Somatic Tissuesmentioning

confidence: 99%

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Use of somatic cell banks in the conservation of wild felids

et al. 2018

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The conservation of biological resources is an interesting strategy for the maintenance of biodiversity, especially for wild felids who are constantly threatened with extinction. For this purpose, cryopreservation techniques have been used for the long-term storage of gametes, embryos, gonadal tissues, and somatic cells and tissues. The establishment of these banks has been suggested as a practical approach to the preservation of species and, when done in tandem with assisted reproductive techniques, could provide the means for reproducing endangered species. Somatic cell banks have been shown remarkable for the conservation of genetic material of felids; by merely obtaining skin samples, it is possible to sample a large group of individuals without being limited by factors such as gender or age. Thus, techniques for somatic tissue recovery, cryopreservation, and in vitro culture of different wild felids have been developed, resulting in a viable method for the conservation of species. One of the most notable conservation programs for wild felines using somatic samples was the one carried out for the Iberian lynx, the most endangered feline in the world. Other wild felids have also been studied in other continents, such as the jaguar in South America. This review aims to present the technical progress achieved in the conservation of somatic cells and tissues in different wild felids, as well address the progress that has been achieved in a few species.

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Section: Sources Of Somatic Tissuesmentioning

confidence: 99%

Section: Sources Of Somatic Tissuesmentioning

confidence: 99%

Use of somatic cell banks in the conservation of wild felids

et al. 2018

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“…Animal cloning by somatic cell nuclear transfer (SCNT) has been the subject of much attention in recent years and as a result, several mammalian species have been cloned [1]. Somatic cell cloning in camelids, however, has proven an especially inefficient technology due to the biological and technical problems and the birth of cloned camels has still only been reported from one laboratory [2].…”

Section: Introductionmentioning

confidence: 99%

Development of a modified method of handmade cloning in dromedary camel

Moulavi

Hosseini

2019

PLoS ONE

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In this study, a modified method of handmade cloning (m-HMC), which had been originally developed in sheep, was used for somatic cell nuclear transfer (SCNT) in the dromedary camel. The unique feature of m-HMC over current SCNT methods lies in the use of a simple device (a finely drawn micropipette made of Pasteur pipette) for chemically-assisted enucleation of oocytes under a stereomicroscope with improved efficiency and ease of operation. Using this system, the throughput of cloned embryo reconstitution was increased over 2-fold compared to the control SCNT method (c-NT). Stepwise measurement of reactive oxygen species (ROS) revealed that method, steps, and duration of SCNT all influenced oxidative activity of oocytes, but their impact were not similar. Specifically, UV-assisted oocyte enucleation was identified as the major source of ROS production, which explained significantly higher total ROS of reconstituted embryos in c-NT compared to m-HMC. Fusion efficiency (95.3±3.3 vs. 75.4±7.6%) and total efficiency of blastocyst development (22.5±3.0 vs. 14.1±4.3%) were significantly higher in m-HMC compared to c-NT, respectively, and blastocysts of transferable quality were obtained in similar rates (41.9±8.2 vs. 48.0±15.2%, respectively). Significance differences were observed in total cell number (155.3±13.6 vs. 123.6±19.5) and trophectoderm (145±9.5 vs. 114.3±15.2), but not inner cell mass (10.3±4.1 vs. 9.3±5.3) counts between blastocysts developed in c-NT compared to m-HMC, respectively. However, expression of key developmental genes ( POU5F1 , KLF4 , SOX2 , MYC , and CDX2 ) was comparable between blastocysts of both groups. The introduced m-HMC method might be a viable approach for efficient production of dromedary camel clones for research and commercial utilization.

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“…Skin cryopreservation comprises a biotechnological tool used to store this organ for long periods at −196°C, aiming for later recovery of fibroblasts and its application in different areas of biological research (Elliott, Wang, & Fuller, ). In general, cells derived from cryopreserved skin can be used in nuclear reprogramming studies for regenerative medicine (Verma, Holland, Temple‐Smith, & Verma, ) and in combination with cloning by somatic cell nuclear transfer for the multiplication of species (Moulavi et al, ). For all these applications, attention to the establishment of biobanks using the skin, especially for species threatened with extinction, has increased in recent years (Tunstall et al, ), as the skin provides a greater recovery of the genetic diversity of the population regardless of gender and age of the individual (León‐Quinto et al, ).…”

Section: Introductionmentioning

confidence: 99%

Quantitative and descriptive histological aspects of jaguar (Panthera onca Linnaeus, 1758) ear skin as a step towards formation of biobanks

Praxedes

Neta

Borges

et al. 2019

Anat Histol Embryol

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Skin of mammals vulnerable to extinction, such as the jaguar, is used as a source of material in conservation strategies. The composition of skin is not uniform among species, and the ability to distinguish similarities in skin morphology in animal groups is fundamental in the application of skin tissue for use in biobanks. The aim of our study was to evaluate the structure, composition and capacity for culture of ear skin from the yellow and black jaguars. Both qualitative and quantitative methods were used, focusing on skin thickness, cell quantification and distribution, collagen density, proliferative activity and viability. Histomorphometrical study of the skin showed a total thickness of 273.2 and 274.6 µm for the yellow and black jaguars, respectively. Melanocytes and fibroblasts were, respectively, 9.7 and 23.0 for the yellow jaguar and 11.3 and 26.8 for the black jaguar. A collagen density of 67.0% and 49.0% was observed for yellow and black jaguars, respectively. Both animals presented a proliferative activity varying between 1.20 and 1.30. All tissues could promote cellular detachment, reaching subconfluence in 10–15 days. This kind of information from histomorphometrical features and cell cultures can be essential for a more targeted application of ear skin cryopreservation in this species, as such information will enable understanding the action of substances on tissues during the conservation process.

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Interspecies somatic cell nuclear transfer in Asiatic cheetah using nuclei derived from post-mortem frozen tissue in absence of cryo-protectant and in vitro matured domestic cat oocytes

Cited by 23 publications

References 42 publications

Use of somatic cell banks in the conservation of wild felids

Use of somatic cell banks in the conservation of wild felids

Development of a modified method of handmade cloning in dromedary camel

Quantitative and descriptive histological aspects of jaguar (Panthera onca Linnaeus, 1758) ear skin as a step towards formation of biobanks

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