Interspecies interaction and effect of co-contaminants in an anaerobic dichloromethane-degrading culture

Trueba-Santiso, Alba; Fernández‐Verdejo, David; Marco‐Rius, Irene; Soder-Walz, Jesica M.; Casabella, Oriol; Vicent, Teresa; Marco‐Urrea, Ernest

doi:10.1016/j.chemosphere.2019.124877

Cited by 21 publications

(8 citation statements)

References 38 publications

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“…9%). Studies with [ 13 C]DCM in D. formicoaceticum detected the 13 C label solely in the methyl group of acetate ([2- 13 C] acetate), congruent with DCM oxidation stopping at formate [ 23 , 35 ], while studies with another Dehalobacterium species in mixed culture that was capable of formate oxidation similarly detected [1,2- 13 C]acetate [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

Novel dichloromethane-fermenting bacteria in the Peptococcaceae family

et al. 2021

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Dichloromethane (DCM; CH2Cl2) is a toxic groundwater pollutant that also has a detrimental effect on atmospheric ozone levels. As a dense non-aqueous phase liquid, DCM migrates vertically through groundwater to low redox zones, yet information on anaerobic microbial DCM transformation remains scarce due to a lack of cultured organisms. We report here the characterisation of DCMF, the dominant organism in an anaerobic enrichment culture (DFE) capable of fermenting DCM to the environmentally benign product acetate. Stable carbon isotope experiments demonstrated that the organism assimilated carbon from DCM and bicarbonate via the Wood–Ljungdahl pathway. DCMF is the first anaerobic DCM-degrading population also shown to metabolise non-chlorinated substrates. It appears to be a methylotroph utilising the Wood–Ljungdahl pathway for metabolism of methyl groups from methanol, choline, and glycine betaine. The flux of these substrates from subsurface environments may either directly (DCM, methanol) or indirectly (choline, glycine betaine) affect the climate. Community profiling and cultivation of cohabiting taxa in culture DFE without DCMF suggest that DCMF is the sole organism in this culture responsible for substrate metabolism, while the cohabitants persist via necromass recycling. Genomic and physiological evidence support placement of DCMF in a novel genus within the Peptococcaceae family, ‘Candidatus Formimonas warabiya’.

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Section: Discussionmentioning

confidence: 99%

Novel dichloromethane-fermenting bacteria in the Peptococcaceae family

et al. 2021

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“…Until now, plenty of contaminants have shown the biodegradability, even the widely used forms of plastics once proven recalcitrant to biodegradation [8,9]. Biodegradation of contaminants is complicated due to the sophisticated interspecies interactions containing cooperation and competition, which will happen in all bioremediation processes like natural attenuation, bio-stimulation, and bioaugmentation [10][11][12][13]. Monitoring biodegradation is then of great importance for understanding the complicated processes and employing appropriate biotechniques for contaminants removal.…”

Section: Introductionmentioning

confidence: 99%

Digital PCR as an Emerging Tool for Monitoring of Microbial Biodegradation

Cao

Dong

et al. 2020

Molecules

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Biodegradation of contaminants is extremely complicated due to unpredictable microbial behaviors. Monitoring of microbial biodegradation drives us to determine (1) the amounts of specific degrading microbes, (2) the abundance, and (3) expression level of relevant functional genes. To this endeavor, the cultivation independent polymerase chain reaction (PCR)-based monitoring technique develops from endpoint PCR, real-time quantitative PCR, and then into novel digital PCR. In this review, we introduce these three categories of PCR techniques and summarize the timely applications of digital PCR and its superiorities than qPCR for biodegradation monitoring. Digital PCR technique, emerging as the most accurately absolute quantification method, can serve as the most promising and robust tool for monitoring of microbial biodegradation.

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“…In the past, several Desulfovibrio sp. or other sulfate-reducing bacteria have been reported in microbial cultures degrading chlorinated compounds [9][10][11][12][13][14] .…”

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confidence: 99%

Transformation of the recalcitrant pesticide chlordecone by Desulfovibrio sp.86 with a switch from ring-opening dechlorination to reductive sulfidation activity

Della-Negra

Chaussonnerie

Fonknechten

et al. 2020

Sci Rep

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The insecticide chlordecone has been used in the French West Indies for decades, resulting in long term pollution, human health problems and social crisis. In addition to bacterial consortia and Citrobacter sp.86 previously described to transform chlordecone into three families of transformation products (A: hydrochlordecones, B: polychloroindenes and C: polychloroindenecarboxylic acids), another bacterium Desulfovibrio sp.86, showing the same abilities has been isolated and its genome was sequenced. Ring-opening dechlorination, leading to A, B and C families, was observed as previously described. changing operating conditions in the presence of chlordecone gave rise to the formation of an unknown sulfur-containing transformation product instead of the aforementioned ones. Its structural elucidation enabled to conclude to a thiol derivative, which corresponds to an undocumented bacterial reductive sulfidation. Microbial experiments pointed out that the chlordecone thiol derivative was observed in anaerobiosis, and required the presence of an electron acceptor containing sulfur or hydrogen sulfide, in a confined atmosphere. It seems that this new reaction is also active on hydrochlordecones, as the 10-monohydrochlordecone A1 was transformed the same way. Moreover, the chlordecone thiol derivative called F1 was detected in several chlordecone contaminated mangrove bed sediments from Martinique Island, highlighting the environmental relevance of these results. Chlordecone is a highly recalcitrant organochlorine pesticide that has been added to the Stockholm convention list of persistent organic pollutants in 2009. Several pest control products containing chlordecone (Kepone, Curlone) or a functionalized derivative (Kelevan) have been manufactured in the US, Brazil and France and applied in the Caribbean, Central America, West Africa, and Europe over an extended period of forty years 1. To date, chlordecone has caused two major environmental disasters: (1) acute exposure of workers at the Hopewell (US) chlordecone production plant in 1975 and massive pollution of the James River, extending over 100 miles that lasted for decades 2 , (2) ongoing impregnation of the French West Indies (FWI) population due to extensive agricultural use of chlordecone from 1972 to 1993 that has resulted in long-term pollution of environmental compartments (soils, water resources, coastal areas) and subsequently of some local food production (vegetables, farmed animals, and seafood). Until now, no remediation strategy has been proven satisfactory in the FWI environment whereas the population has to deal with health troubles (increased risk of cancer, motor and cognitive development disorders in young children, premature births) and social difficulties (disappearance of local economy activities) 1 .

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Interspecies interaction and effect of co-contaminants in an anaerobic dichloromethane-degrading culture

Cited by 21 publications

References 38 publications

Novel dichloromethane-fermenting bacteria in the Peptococcaceae family

Novel dichloromethane-fermenting bacteria in the Peptococcaceae family

Digital PCR as an Emerging Tool for Monitoring of Microbial Biodegradation

Transformation of the recalcitrant pesticide chlordecone by Desulfovibrio sp.86 with a switch from ring-opening dechlorination to reductive sulfidation activity

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