Interspecies gene transfer provides soybean resistance to a fungal pathogen

Langenbach, Caspar; Schultheiß, Holger; Rosendahl, Martin; Tresch, Nadine; Conrath, Uwe; Goellner, Katharina

doi:10.1111/pbi.12418

Cited by 40 publications

(37 citation statements)

References 46 publications

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“…Similarly, the rice receptor-like kinase (RLK) gene Xa21 has been shown to provide Banana Xanthomonas wilt resistance in banana (Tripathi et al 2014). The nonhost resistance NHR-linked Arabidopsis genes PING 4 (coding for a phospholipase-like protein (EARLI4-like)), PING 5 (coding for a leucine-rich repeat protein kinase) and PING 6 (coding for an ankyrin repeat family protein), known to provide pre-invasion resistance to nonadapted fungal pathogens, have been transferred into soya bean where they confer Asian soya bean rust resistance (Langenbach et al, 2016). Lr34res also has been successfully transferred in other crop species such as barley and rice Risk et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

The durable wheat disease resistance gene Lr34 confers common rust and northern corn leaf blight resistance in maize

Sucher

Böni

Yang

et al. 2016

Plant Biotechnology Journal

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SummaryMaize (corn) is one of the most widely grown cereal crops globally. Fungal diseases of maize cause significant economic damage by reducing maize yields and by increasing input costs for disease management. The most sustainable control of maize diseases is through the release and planting of maize cultivars with durable disease resistance. The wheat gene Lr34 provides durable and partial field resistance against multiple fungal diseases of wheat, including three wheat rust pathogens and wheat powdery mildew. Because of its unique qualities, Lr34 became a cornerstone in many wheat disease resistance programmes. The Lr34 resistance is encoded by a rare variant of an ATP‐binding cassette (ABC) transporter that evolved after wheat domestication. An Lr34‐like disease resistance phenotype has not been reported in other cereal species, including maize. Here, we transformed the Lr34 resistance gene into the maize hybrid Hi‐II. Lr34‐expressing maize plants showed increased resistance against the biotrophic fungal disease common rust and the hemi‐biotrophic disease northern corn leaf blight. Furthermore, the Lr34‐expressing maize plants developed a late leaf tip necrosis phenotype, without negative impact on plant growth. With this and previous reports, it could be shown that Lr34 is effective against various biotrophic and hemi‐biotrophic diseases that collectively parasitize all major cereal crop species.

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Section: Discussionmentioning

confidence: 99%

The durable wheat disease resistance gene Lr34 confers common rust and northern corn leaf blight resistance in maize

Sucher

Böni

Yang

et al. 2016

Plant Biotechnology Journal

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“…Several studies have demonstrated the feasibility of transferring single NHR-related genes across plant species to create durable, broad-spectrum resistance (Brutus and He, 2010; Wen et al, 2011; Schoonbeek et al, 2015; Langenbach et al, 2016). The mechanisms of rice resistance to Pst can be used to improve wheat resistance.…”

Section: Discussionmentioning

confidence: 99%

“…For example, NHR of Arabidopsis and Medicago to bean rust (Patto and Rubiales, 2014; Ishiga et al, 2015; Langenbach et al, 2016), barley and Brachypodium distachyon NHR to cereal rust pathogens (Zellerhoff et al, 2010; Dawson et al, 2015; Figueroa et al, 2015; Niks et al, 2015a). As the model of monocotyledonous plant, rice is unusual in not being affected by a rust pathogen (Ayliffe et al, 2011b).…”

Section: Introductionmentioning

confidence: 99%

Characterization and Genetic Analysis of Rice Mutant crr1 Exhibiting Compromised Non-host Resistance to Puccinia striiformis f. sp. tritici (Pst)

et al. 2016

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Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most devastating diseases of wheat in China. Rapid change to virulence following release of resistant cultivars necessitates ongoing discovery and exploitation of new resistance resources. Considerable effort has been directed at non-host resistance (NHR) which is believed to be durable. In the present study we identified rice mutant crr1 (compromised resistance to rust 1) that exhibited compromised NHR to Pst. Compared with wild type rice variety Nipponbare, crr1 mutant displayed a threefold increase in penetration rate by Pst, and enhanced hyphal growth. The pathogen also developed haustoria in crr1 mesophyll cells, but failed to sporulate. The response to the adapted rice pathogen Magnaporthe oryzae was unchanged in crr1 relative to the wild type. Several defense-related genes involved in the SA- and JA-mediated defense pathways response and in phytoalexin synthesis (such as OsPR1a, OsLOX1, and OsCPS4) were more rapidly and strongly induced in infected crr1 leaves than in the wild type, suggesting that other layers of defense are still in effect. Genetic analysis and mapping located the mutant loci at a region between markers ID14 and RM25792, which cover about 290 kb genome sequence on chromosome 10. Further fine mapping and cloning of the locus should provide further insights into NHR to rust fungi in rice, and may reveal new strategies for improving rust resistance in wheat.

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“…To compare the activation pattern of PPP routes in the Arabidopsis non-host and soybean host we first monitored the expression of genes potentially involved in scopoletin and flavonoid biosynthesis in the SBR-susceptible soybean cultivar Williams 82 (W82) after inoculation with the highly virulent Pp isolate BR05 (Langenbach et al, 2016b). Consistent with an earlier report (Schneider et al, 2011) we detected biphasic induction of PPP genes in the soybean PAL, C4H and CHS families with an early expression peak at 12-24 h post inoculation (hpi) and a late expression maximum at 144-216 hpi ( Figure 2).…”

Section: Scopoletin Is Absent From Leaves Of Both Sbr-susceptible Andmentioning

confidence: 99%

The Arabidopsis non‐host defence‐associated coumarin scopoletin protects soybean from Asian soybean rust

et al. 2019

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Summary The fungus Phakopsora pachyrhizi (Pp) causes Asian soybean rust (SBR) disease which provokes tremendous losses in global soybean production. Pp is mainly controlled with synthetic fungicides to which the fungus swiftly develops fungicide resistance. To substitute or complement synthetic fungicides in Asian soybean rust control, we aimed to identify antifungal metabolites in Arabidopsis which is not a host for Pp. Comparative transcriptional and metabolic profiling of the Pp‐inoculated Arabidopsis non‐host and the soybean host revealed induction of phenylpropanoid metabolism‐associated genes in both species but activation of scopoletin biosynthesis only in the resistant non‐host. Scopoletin is a coumarin and an antioxidant. In vitro experiments disclosed fungistatic activity of scopoletin against Pp, associated with reduced accumulation of reactive oxygen species (ROS) in fungal pre‐infection structures. Non‐antioxidant and antioxidant molecules including coumarins with a similar structure to scopoletin were inactive or much less effective at inhibiting fungal accumulation of ROS and germination of Pp spores. When sprayed onto Arabidopsis leaves, scopoletin also suppressed the formation of Pp pre‐infection structures and penetration of the plant. However, scopoletin neither directly activated defence nor did it prime Arabidopsis for enhanced defence, therefore emphasizing fungistatic activity as the exclusive mode of action of scopoletin against Pp. Because scopletin also protected soybean from Pp infection, the coumarin may serve as a natural fungicide or as a lead for the development of near‐to‐nature fungicides against Asian soybean rust.

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Interspecies gene transfer provides soybean resistance to a fungal pathogen

Cited by 40 publications

References 46 publications

The durable wheat disease resistance gene Lr34 confers common rust and northern corn leaf blight resistance in maize

The durable wheat disease resistance gene Lr34 confers common rust and northern corn leaf blight resistance in maize

Characterization and Genetic Analysis of Rice Mutant crr1 Exhibiting Compromised Non-host Resistance to Puccinia striiformis f. sp. tritici (Pst)

The Arabidopsis non‐host defence‐associated coumarin scopoletin protects soybean from Asian soybean rust

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