Interspecies cross‐talk between co‐cultured <i>Pseudomonas putida</i> and <i>Escherichia coli</i>

Molina-Santiago, Carlos; Udaondo, Zulema; Cordero, Baldo F.; Ramos, Juan L.

doi:10.1111/1758-2229.12553

Cited by 11 publications

(7 citation statements)

References 45 publications

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“…It is emphasized that acetate reduced the inhibitory effect of indole on the TGC02 and TGC04 isolates when the two molecules were associated, especially under conditions where the indole concentration was 0.25 mM, as observed at acetate 2 mM and indole 0.5 mM, when TGC02 produced 68 μg/mL of pyocyanin, representing an increase of about 20% over the control. This apparent equilibrium suggests that acetate may have been used as an additional carbon source, based on a previous observation of the use of acetate by a P. putida strain in the presence of E. coli DOT-T1E [28].…”

Section: Fig 1: Influence Of Indole and Acetate On The Production Of Pyocyanin By Pseudomonas Aeruginosa Tgc02 (A) And Tgc04 (B) The Colomentioning

confidence: 74%

“…This association sometimes represents disturbances especially to coliforms, although with a biostatic rather than a biocidal effect [2,27]. In order avoid disturbance in the cells, E. coli releases secondary metabolites to keep itself viable in the environment, until it finds favourable conditions for regrowth [11,28].…”

Section: Fig 1: Influence Of Indole and Acetate On The Production Of Pyocyanin By Pseudomonas Aeruginosa Tgc02 (A) And Tgc04 (B) The Colomentioning

confidence: 99%

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Activity of two Exometabolites produced by Escherichia coli on the Synthesis of Pyocyanin

Arruda¹,

Oliveira²,

Bonifácio³

et al. 2019

IJAERS

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The secretion of metabolites with antimicrobial activity is one of the strategies employed by bacteria to respond to negative stimuli promoted during interspecies competition. In a long -term stationary phase. Pseudomonas aeruginosa and Escherichia coli can synthesize diffusible exometabolites whose action is to mutually inhibit the exposed cells, guaranteeing the balance of both populations in a certain site. The P. aeruginosa may have an advantage in that it produces pyocyanin. Howeve r, the excretion of indole and acetate by E. coli may reduce this advantage. This work aims to detect the influence of different concentrations of these two exometabolites on the synthesis of pyocyanin in two wild isolates of P. aeruginosa. After incubatio n under shaking for 72 h at 29°C, reduction of up to 50% of the concentration of pyocyanin in the presence of indole was observed. On the other hand, no change was observed in the production of the pigment with the acetate, alone or when in combination with concentrations of less than 0.5 mM indole. It reduced the inhibitory effect of the compound, reflecting an increase in pyocyanin production of more than 20%. The results contribute to help understanding the ecological mechanisms of competition between t he two species.

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Section: Fig 1: Influence Of Indole and Acetate On The Production Of Pyocyanin By Pseudomonas Aeruginosa Tgc02 (A) And Tgc04 (B) The Colomentioning

confidence: 74%

Section: Fig 1: Influence Of Indole and Acetate On The Production Of Pyocyanin By Pseudomonas Aeruginosa Tgc02 (A) And Tgc04 (B) The Colomentioning

confidence: 99%

Activity of two Exometabolites produced by Escherichia coli on the Synthesis of Pyocyanin

Arruda¹,

Oliveira²,

Bonifácio³

et al. 2019

IJAERS

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“…In fact, E. coli-Salmonella co-culture has been shown to increase biofilm formation and lead to an increased biocide resistance [ 118 ]. In some co-cultures, an increased efflux pump expression is observed, possibly as a defense mechanism against the other species, representing a shift from co-operative interaction to competitive exclusion [ 119 ]. However, in some instances, these “cheaters” do not benefit from producers, as producers exert a quorum control over efflux pumps when antagonized with certain antibiotics [ 120 ].…”

Section: Efflux Pumps and Polymicrobial Communitiesmentioning

confidence: 99%

Efflux, Signaling and Warfare in a Polymicrobial World

et al. 2023

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The discovery void of antimicrobial development has occurred at a time when the world has seen a rapid emergence and spread of antimicrobial resistance, the ‘perfect storm’ as it has often been described. While the discovery and development of new antibiotics has continued in the research sphere, the pipeline to clinic has largely been fed by derivatives of existing classes of antibiotics, each prone to pre-existing resistance mechanisms. A novel approach to infection management has come from the ecological perspective whereby microbial networks and evolved communities already possess small molecular capabilities for pathogen control. The spatiotemporal nature of microbial interactions is such that mutualism and parasitism are often two ends of the same stick. Small molecule efflux inhibitors can directly target antibiotic efflux, a primary resistance mechanism adopted by many species of bacteria and fungi. However, a much broader anti-infective capability resides within the action of these inhibitors, borne from the role of efflux in key physiological and virulence processes, including biofilm formation, toxin efflux, and stress management. Understanding how these behaviors manifest within complex polymicrobial communities is key to unlocking the full potential of the advanced repertoires of efflux inhibitors.

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“…Moreover, it is important to study interactions among multiple organisms, especially within microbial ecosystems [10] for several reasons: 1) how their pathways are interconnected, 2) how their interactions changes due the perturbation of one or both of their systems, 3) how do they correspond to external factors such environmental changes or treatment stimuli, etc. Hence, more research required to determine and characterize these inter-species pathway cross-talks to reveal better insights into the networks of diseases-associated biological pathways in a data-driven manner.…”

Section: Introductionmentioning

confidence: 99%

XTalkiiS: a tool for finding data-driven cross-talks between intra-/inter-species pathways

Azad

2018

Preprint

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Cell-cell communication via pathway cross-talks within a single species have been studied in silico recently to decipher various disease phenotype. However, computational prediction of pathway cross-talks among multiple species in a data-driven manner is yet to be explored. In this article, I present XTalkiiS (Cross-talks between inter-/intra species pathways), a tool to automatically predict pathway cross-talks from data-driven models of pathway network, both within the same organism (intra-species) and between two organisms (inter-species). XTalkiiS starts with retrieving and listing up-to-date pathway information in all the species available in KEGG database using RESTful APIs (exploiting KEGG web services) and an in-house built web crawler. I hypothesize that data-driven network models can be built by simultaneously quantifying co-expression of pathway components (i.e. genes/proteins) in matched samples in multiple organisms. Next, XTalkiiS loads a data-driven pathway network and applies a novel cross-talk modelling approach to determine interactions among known KEGG pathways in selected organisms. The potentials of XTalkiiS are huge as it paves the way of finding novel insights into mechanisms how pathways from two species (ideally host-parasite) may interact that may contribute to the various phenotype of interests such as malaria disease. XTalkiiS is made open sourced at https://github.com/Akmazad/XTalkiiS and its binary files are freely available for downloading from https://sourceforge.net/projects/xtalkiis/. 129 knowledgebase.

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Interspecies cross‐talk between co‐cultured Pseudomonas putida and Escherichia coli

Cited by 11 publications

References 45 publications

Activity of two Exometabolites produced by Escherichia coli on the Synthesis of Pyocyanin

Activity of two Exometabolites produced by Escherichia coli on the Synthesis of Pyocyanin

Efflux, Signaling and Warfare in a Polymicrobial World

XTalkiiS: a tool for finding data-driven cross-talks between intra-/inter-species pathways

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