Interspecies Comparison of Phagolysosomal pH in Alveolar Macrophages

Kreyling, Wolfgang G.; Nyberg, Kristina; Nolibé, D.; Collier, C. G.; Camner, Per; Heilmann, P.; Lirsac, Pierre-Noël; Lundborg, Margot; Matějková, Eva

doi:10.3109/08958379109145276

Cited by 33 publications

(18 citation statements)

References 14 publications

(23 reference statements)

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“…Phagolysosomal pH is around 5 for many species including humans (Kreyling et al, 1991;Nyberg et al, 1994). Our results showed that most phagolysosomes containing conidia of A. fumigatus had a pH <5.5.…”

Section: Discussionmentioning

confidence: 64%

Reaction of Human Alveolar Macrophages to Exposure toAspergillus fumigatusand Inert Particles

Nessa

Palmberg²,

Johard

et al. 1997

Environmental Research

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Section: Discussionmentioning

confidence: 64%

Reaction of Human Alveolar Macrophages to Exposure toAspergillus fumigatusand Inert Particles

Nessa

Palmberg²,

Johard

et al. 1997

Environmental Research

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“…phagolysosomes of the macrophages, where 0(a) the pH is approximately 4.8 (12). Two days after instillation, the number of wollastonite fibers in the lung was about 100 to 200 x 106.…”

Section: Methodsmentioning

confidence: 97%

Investigation of the Biodurability of Wollastonite and Xonotlite

Bellmann¹,

Muhle²

1994

Environmental Health Perspectives

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The in vivo durability of wollastonite materials, coated and uncoated, and of xonotlite was tested. Wollastonite is an anhydrous natural silicate and xonotlite is a hydrated synthetic calcium silicate. UICC crocidolite was used as a positive control with high durability. Using a dry-sizing technique, fractions from the stock materials were prepared according to the definition of "thoracic particulate mass" and "respirable particulate mass" of the American Conference of Governmental Industrial Hygienists. Fibers were instilled intratracheally into female Wistar rats, and the evenness of their distribution in the lung was checked by scanning electron microscopy (SEM). After serial sacrifices at 2 and 14 days, 1, 3, and 6 months, and low temperature ashing of the lung, the fibers were analyzed by SEM. The number and size distribution of fibers were investigated. The total number of crocidolite fibers decreased with a half-time of 240 days, but the number of fibers >5 pm in length was unchanged after 6 months. The elimination kinetics of wollastonite fibers from the lung were relatively fast, with half-times of 15 to 21 days. The coating of wollastonite in Wollastocoat had no effect on this elimination process. For the thoracic fraction of wollastonite, the elimination from the lung was as fast as for the respirable particulate fraction. The elimination kinetics of xonotlite from the lung was very fast. This material consisted of single crystals of acicular morphology with a median length of 1.3 pm and of agglomerates of these crystals. More than 99% of single crystals and about 85 to 89% of the agglomerates were already eliminated 2 days after instillation. -Environ Health Perspect 102 (Suppl 5):191-195 (1994)

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“…Phagolysosomal pH is around 5 for many species including human (Nyberg et al, 1989;Kreyling et al, 1991). Klebanoff (1982) reported that the ferrous iron-hydrogen peroxide-halide system, which has a potent fungicidal activity, acts rapidly and optimally at a low pH within the phagolysosomes.…”

Section: Figmentioning

confidence: 99%