Interspecies comparison of mucosal wave properties using high‐speed digital imaging

Abstract

Objectives The purpose of this study was to compare the vocal fold vibratory characteristics of ex vivo bovine, canine, ovine, and porcine larynges to human male and female vocal fold vibrations to determine the best model organism for laryngeal studies concerning vibratory and kinetic characteristics. Study Design Prospective experimental Methods High speed videos were gathered at 4000 frames per second (fps) in the animal models and human high-speed endoscopy data were gathered at 2000 fps. Videos were converted into kymograms and the amplitude, oscillation frequency, and phase difference of vocal fold vibration were measured. Results No statistically significant differences were found with respect to frequency, amplitude, or phase difference between canines and humans. Swine were not significantly different from human females but did have a significantly different oscillation frequency than human males. Ovine vibrational amplitudes were significantly different from humans, and bovine frequency and amplitude differed significantly from humans. Conclusion Canine and porcine larynges are the most appropriate model specimens for laryngeal studies contingent on vibratory or kinetic properties of phonation.

Young's Modulus of Canine Vocal Fold Cover Layers

“…Mucosal wave properties of the larynx was compared by Regner et al between ex vivo bovine, canine, ovine, and porcine laryngeal vibration and in vivo human laryngeal vibration using high speed digital imaging. 18 They reported that “no statistically significant differences were found with respect to frequency, amplitude, or phase difference between canines and humans. Porcines were not significantly different from human females but did have an oscillation frequency significantly different from human males.…”

High-Frequency Viscoelastic Shear Properties of Vocal Fold Tissues: Implications for Vocal Fold Tissue Engineering

“…Porcine VFs are the focus of this work owing to their structural similarity to human VFs [30][31][32][33][34][35] and the availability of tissue samples of varying age and gender. The LP of 38 pigs, varying in age from fetal to 2 + -year olds, were tested using the TWE.…”

Excised larynx experimentation: history, current developments, and prospects for bioacoustic research

“…Surprisingly, in a large number of these experiments, larynges of various mammalian species (e.g. dogs, pigs, sheep, cows, rabbits, or rats (Alipour and Jaiswal, 2008;Welham et al, 2009;Regner et al, 2010;Maytag et al, 2013)) were utilized as a proxy for the human vocal organ, probably for ethical reasons. It is sometimes assumed that the vocal fold vibratory characteristics and sound output data from these species are largely comparable with those of their human counterpart (Jiang et al, 2001a;Regner et al, 2010;Alipour et al, 2013).…”

“…dogs, pigs, sheep, cows, rabbits, or rats (Alipour and Jaiswal, 2008;Welham et al, 2009;Regner et al, 2010;Maytag et al, 2013)) were utilized as a proxy for the human vocal organ, probably for ethical reasons. It is sometimes assumed that the vocal fold vibratory characteristics and sound output data from these species are largely comparable with those of their human counterpart (Jiang et al, 2001a;Regner et al, 2010;Alipour et al, 2013). It should, however, be taken into consideration that the anatomical layout of the vocal folds (particularly that of the lamina propria) differs between these species and humans (Kurita et al, 1983;Garrett et al, 2000), thus potentially leading to different biomechanical properties of the vocal folds and consequently resulting in diverse vibratory characteristics and sound output.…”