Intersimple Sequence Repeat Fingerprinting and Genetic Variation in a Collection of Clematis Cultivars and Commercial Germplasm

Gardner, Nicole; Hokanson, Stan C.

doi:10.21273/hortsci.40.7.1982

Cited by 15 publications

(10 citation statements)

References 35 publications

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“…ISSR markers have been used successfully to reveal information about lowbush blueberries. The results clearly demonstrate that ISSR markers can be used in a genetic diversity study as well as in genotypic identification of lowbush blueberries, as noted for other plant species by Prevost and Wilkinson (1999), Gardner and Hokanson (2005), Debnath (2007a), , Yakimowski and Eckert (2008) and Yao et al (2008).…”

Section: Discussionsupporting

confidence: 56%

“…Each marker system has its own strengths and limitations, making the choice of marker an important decision. Inter simple sequence repeat markers (ISSRs) (Zietkiewicz et al 1994) have been used successfully on a number of horticultural species including potato (Prevost and Wilkinson 1999), Clematis species (Gardner and Hokanson 2005), lingonberry Vaccinium vitis‐idaea L. (Debnath 2007a), deerberry V. stamineum L. (Yakimowski and Eckert 2008) and strawberry Fragaria cultivars (Arnau et al 2002, Korbin et al 2002, Debnath et al 2008). The ISSR primers target microsatellites that are abundant throughout the plant genome (Wang et al 1994).…”

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confidence: 99%

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Development of ISSR markers for genetic diversity studies in Vaccinium angustifolium

Debnath¹

2009

Nordic Journal of Botany

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Understanding of the genetic relationship within wild lowbush blueberry (Vaccinium angustifolium Ait.) germplasm is important to establish a broad genetic base for safeguarding and for future use of the existing genetic resources. The objective of this study was to assess the genetic variability within 43 wild lowbush blueberry clones, collected from 10 communities of four Canadian provinces, and the cultivar ‘Fundy’ by using inter simple sequence repeat (ISSR) markers, with the hope to establish a reference set of lowbush blueberry germplasm for blueberry conservation, breeding and research. Thirteen primers generated 242 polymorphic ISSR‐PCR bands. A substantial degree of genetic similarity was found among the wild collections. Cluster analysis by the unweighted pair‐group method with arithmetic averages (UPGMA) separated the 41 genotypes into two main clusters, and identified the three remaining clones as outliers. Furthermore, within one main cluster, the genotypes tended to form sub‐clusters that were in agreement with the principal coordinate (PCO) analysis. Geographical distribution contributed to 27% of total variation as revealed by analysis of molecular variance (AMOVA). The ISSR‐PCR method was simple, fast and relatively inexpensive to produce useful DNA fragments and detected a sufficient degree of polymorphism to differentiate among lowbush blueberry clones, making this technology valuable for germplasm management and the more efficient choice of parents in current blueberry breeding program.

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Section: Discussionsupporting

confidence: 56%

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confidence: 99%

Development of ISSR markers for genetic diversity studies in Vaccinium angustifolium

Debnath¹

2009

Nordic Journal of Botany

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“…Cerbah et al (2001 and, more recently, Mortreau et al (2010) revealed significant differences in the chromosome organization between these two sub-species, that includes the ploidy level and the quantity of DNA, as well as heterochromatin organization and ribosomic sequences. ISSR markers have been successfully used for varietal identification, estimation of genetic relationships and genetic diversity in many ornamental species such as clematis (Gardner and Hokanson 2005), tree peony (Suo et al 2005), leucadendron (Pharmawati et al 2005), chrysanthemum (Chatterjee et al 2006) and rose (Crespel et al 2009). In our study, the polymorphism revealed by ISSR markers between the two sub-species is high (96.9%) and is in agreement with the high level of intraspecific variation already revealed in H. aspera at the molecular level by Mortreau et al (2003).…”

Section: Discussionmentioning

confidence: 99%

“…Similar ISSR polymorphism levels have already been reported in other genera, but at the interspecific level. For example, the proportion of polymorphic markers is 94.0% between five species of the genus Clematis, 99.5% between 12 species of the genus Grevillea and 98.1% between nine species of the genus Jatropha (Gardner and Hokanson 2005;Pharmawati et al 2004;Senthil Kumar et al 2009). The fact that Jaccard dissimilarity index is so high between the two subspecies of H. aspera (0.97) clearly reveals their considerable genetic differentiation.…”

Section: Discussionmentioning

confidence: 99%

Architectural and genetic characterization of Hydrangea aspera subsp. aspera Kawakami group, H. aspera subsp. sargentiana and their hybrids

Crespel

Morel²,

Galopin

2011

Euphytica

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Hydrangea macrophylla (Thunb.) Ser. and H. paniculata Sieb. are the two most economically important species within the genus Hydrangea, and have been used as ornamental garden plants for a long time. However, other species such as H. aspera D. Don are of horticultural interest, particularly for the color of their inflorescences and plant shape. This species is composed of four sub-species and has previously been characterized both genetically and morphologically. The previous morphological characterization was qualitative, but was based mainly on leaf and inflorescence parameters outlined by UPOV, and provided little information about plant shape. To better characterize the shape of H. aspera, an architectural analysis was applied to the two most distantly related sub-species at the cytogenetic level:subsp. sargentiana (Redher) E.M. McClint. (clone 188) and subsp. aspera Kawakami group (clone 352). This method made it possible to reveal significant differences between these clones, both at the axis and the growth unit (GU) scale, in agreement with the high level of genetic differentiation (Jaccard dissimilarity index equal to 0.97) revealed between the two clones by Inter simple sequence repeats markers. Because this method is difficult to apply to a large population of individuals, a qualitative architectural characterization was tested on ten progenies derived from hybridization of the two clones, on the basis of their most discriminating architectural components. The hybrid nature of the progeny was confirmed by the architectural analysis. The architectural components of the hybrids are therefore a combination of those of the parents, with a predominance of clone 352, the female parent. Architectural differences between hybrids were clearly revealed by the length of the first vegetative GU (VGU1), the presence or the absence of VGU2 and the length of the floral GU of the A2 axis, and GU branching, allowing us to define five architectural profiles. These differences are supported by the average Jaccard dissimilarity index (0.33). This method, based on a qualitative description of the main architectural components of the plant, proved to be useful for characterizing the shape of H. aspera subsp. sargentiana, and subsp. aspera Kawakami group, and their hybrids. It could be extended to other sub-species of H. aspera and to their respective hybrids, providing an efficient L. Crespel (

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“…ISSRs cost less and are easier to use than amplified fragmentlength polymorphisms (AFLPs) and do not require prior knowledge of flanking sequences, like SSRs (Reddy et al, 2002). ISSR markers are thought to be particularly useful for study of closely related individuals that exhibit low levels of polymorphism (Zietkiewicz et al, 1994) and have been applied as a very useful alternative to fingerprinting and genetic analysis in fruit crops including strawberry (Debnath et al, 2008), raspberry (Debnath, 2007a), clematis (Gardner and Hokanson, 2005), and plum (Liu et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity, Antioxidant Activities, and Anthocyanin Contents in Lingonberry

Debnath

Sion

2009

International Journal of Fruit Science

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Lingonberry (Vaccinium vitis-idaea L.) wild clones and cultivarswere assessed for antioxidant activities, anthocyanin content, and for genetic variability using inter-simple sequence repeat (ISSR) markers. Four ISSR primers generated 113 polymorphic bands in 34 clones and eight cultivars. Cluster analysis by the unweighted pair-group method with arithmetic averages (UPGMA) separated the 41 genotypes into three main clusters, and identified the one remaining clone as an outlier. Within one cluster, the genotypes tended to form subclusters that were in agreement with a principal coordinate (PCO) analysis. Geographical distribution based on country of collection explained 12% of the total variation as revealed by analysis of molecular variance (AMOVA). Antioxidant activity and anthocyanin content were higher in the berries of clones belonging to V. vitis-idaea ssp. minus than those of the V. vitis-idaea ssp. vitis-idaea cultivars. The UPGMA clustering for chemical markers with 11 clones and seven cultivars identified two major clusters and one outlier. The ISSR markers and analyses of antioxidant activities and anthocyanin contents detected a sufficient degree of polymorphism to differentiate among lingonberries, making this technology valuable for germplasm management, and more efficient choices of parents in current lingonberry breeding programs.

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Intersimple Sequence Repeat Fingerprinting and Genetic Variation in a Collection of Clematis Cultivars and Commercial Germplasm

Cited by 15 publications

References 35 publications

Development of ISSR markers for genetic diversity studies in Vaccinium angustifolium

Development of ISSR markers for genetic diversity studies in Vaccinium angustifolium

Architectural and genetic characterization of Hydrangea aspera subsp. aspera Kawakami group, H. aspera subsp. sargentiana and their hybrids

Genetic Diversity, Antioxidant Activities, and Anthocyanin Contents in Lingonberry

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