Intersexual variations in the venom of the Brazilian ‘armed’ spider Phoneutria nigriventer (Keyserling, 1891)

Herzig, Volker; Ward, Richard J.; Santos, Wagner Ferreira dos

doi:10.1016/s0041-0101(02)00136-8

Cited by 40 publications

(34 citation statements)

References 18 publications

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“…However, intraspecific variations can also occur as shown recently in Dinoponera quadriceps for which only 48 peptides were shared between colonies out of the more than 300 peptides found in total (Cologna et al 2013). Also, intraspecific variations in venom composition have been observed in snakes, scorpions, tarantulas and cone snails, this variation being linked to geographical distribution (Núñez et al 2009;Shashidharamurthy et al 2002), age (Escoubas et al 2002) or sex (Escoubas et al 1997;Herzig and Hodgson 2009;Herzig et al 2002;Herzig et al 2008). However, it remains unclear whether such variation is a common denominator for all venomous animals or is restricted to some taxa or species.…”

Section: Introductionmentioning

confidence: 86%

Intraspecific variations in the venom peptidome of the ant Odontomachus haematodus (Formicidae: Ponerinae) from French Guiana

Touchard¹,

Déjean²,

Escoubas³

et al. 2015

JHR

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Ant venoms are complex cocktails of toxins employed to subdue prey and to protect the colony from predators and microbial pathogens. Although the extent of ant venom peptide diversity remains largely unexplored, previous studies have revealed the presence of numerous bioactive peptides in most stinging ant venoms. We investigated the venom peptidome of the ponerine ant Odontomachus haematodus using LC-MS analysis and then verified whether the division of labor in the colonies and their geographical location are correlated with differences in venom composition. Our results reveal that O. haematodus venom is comprised of 105 small linear peptides. The venom composition does not vary between the different castes (i.e., nurses, foragers and queens), but an intraspecific variation in peptide content was observed, particularly when the colonies are separated by large distances. Geographical variation appears to increase the venom peptide repertoire of this ant species, demonstrating its intraspecific venom plasticity.

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Section: Introductionmentioning

confidence: 86%

Intraspecific variations in the venom peptidome of the ant Odontomachus haematodus (Formicidae: Ponerinae) from French Guiana

Touchard¹,

Déjean²,

Escoubas³

et al. 2015

JHR

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“…Brießy, 15-to 20-V electrical stimuli were repeatedly applied to the spider sternum, and the venom drops were collected with a micropipette, vacuum dried, and stored at Ϫ20ЊC. Only female venom was used, because intersexual variations in the toxic potential of P. nigriventer have already been shown; female venom is more poisonous than male venom (Herzig et al 2002). The protein content of the samples was evaluated by the method of Smith et al (1985), using the BCA Protein Assay kit (Pierce Biotechnology, Rockford, IL).…”

Section: Methodsmentioning

confidence: 99%

Ctenus mediusandPhoneutria nigriventerSpiders Venoms Share Noxious Proinflammatory Activities

et al. 2009

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Ctenus medius Keyserling, 1891 (Araneae: Ctenidae) co-occurs in various microhabitats of the Brazilian Atlantic Forest and can be easily misidentified as the medically important spider Phoneutria nigriventer Keyserling, 1981 (Ctenidae). Despite being phylogenetically close to Phoneutria, no data are available about the toxic potential of Ctenus medius venom. Here we show that, although presenting different profile of protein composition, C. medius venom displays some of the toxic properties exhibited by P. nigriventer venom, including proteolytic, hyaluronidasic and phospholipasic activities, as well as the ability of causing hyperalgesia and edema. Moreover, C. medius venom interferes in the activation of the complement system in concentrations that P. nigriventer venom is inactive. Thus, these data show that venoms of spiders from Ctenidae family share important proinflammatory properties and suggest that the C. medius bite may have an important noxious effect in human accidents.

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“…Spiders in the Ctenidae family, a group containing nearly 500 species in 42 genera that range mostly in tropical terrains, is home to the most venomous spider in the world Phoneutria nigriventer [11], and a nonlethal spider that has become the model species for arachnological studies on evolution and development Cuppienius salei [12]; both of which are South American spiders whose venom has been highly studied [13,14]. In the U.S., Ctenus hibernalis is one of only 7 representative species of Ctenidae spiders and it has primarily been collected in Alabama [15], but little to no information is available about its ecology or physiology, nor is there anything known about its divergence from its tropical counterparts in relation to its venom.…”

Section: Introductionmentioning

confidence: 99%

Characterization of the Venom Proteome for the Wandering Spider, Ctenus hibernalis (Aranea: Ctenidae)

Cole¹,

Buszka²,

Mobley³

et al. 2016

J Proteomics Bioinform

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Spider venom is a rich multicomponent mixture of neurotoxic polypeptides. The venom of a small percentage of the currently classified spiders has been categorized. In order to determine what venom proteins are expressed in our species, the wandering spider Ctenus hibernalis, we constructed a comprehensive proteome derived from a crude venom extract using a GeLC approach that required a one dimensional denatured gel electrophoresis separation combined with enzymatic digestion of the entire lane cut into many molecular weight fractions followed by LC-ESI-MS2. In this way, we identified 1,182 proteins with >99% confidence that closely matched sequences derived from the combined genomes taken from several similar species of spiders. Our results suggest that the venom proteins of C. hibernalis contain several proteins with conserved sequences similar to other species. Going forward, with next generation sequencing (NSG), combined with extended annotations will be used to construct a more complete genoproteomic database. Therefore, it is expected that with further studies like this, there will be a continued and growing understand of the genoproteomic makeup of the venom for many species derived from insects, plants, and animals. We believe that as a whole these approaches will lead to a much better understanding of the biology behind venoms of all types, as well as ways to treat exposed patients while also expanding upon and taking advantage of the various positive sides of venomous toxins.Citation: Cole J, Buszka PA, Mobley JA, Hataway RA (2016) Characterization of the Venom Proteome for the Wandering Spider, Ctenus hibernalis (Aranea: Ctenidae).

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Intersexual variations in the venom of the Brazilian ‘armed’ spider Phoneutria nigriventer (Keyserling, 1891)

Cited by 40 publications

References 18 publications

Intraspecific variations in the venom peptidome of the ant Odontomachus haematodus (Formicidae: Ponerinae) from French Guiana

Intraspecific variations in the venom peptidome of the ant Odontomachus haematodus (Formicidae: Ponerinae) from French Guiana

Ctenus mediusandPhoneutria nigriventerSpiders Venoms Share Noxious Proinflammatory Activities

Characterization of the Venom Proteome for the Wandering Spider, Ctenus hibernalis (Aranea: Ctenidae)

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