The increasing technological evolution in hospitals, combined with stressful and burdensome working conditions causes distress in nursing professionals. These elements suggest an increase in the physiological workload of workers in this service. This paper aims to identify the parameters or variables used to estimate the physiological workload of dynamic tasks in hospital workers, as well as the methods used to measure it and their specificities. A systematic literature review was performed using the PRISMA protocol, in journal databases such as Scopus, Science Direct, and Pubmed Central. Our searches retrieved 1321 articles and, after classification, we selected 35 articles for full analysis. The results showed a lack of consensual physiological workload definition, although there are several parameters and methods to measure it. Finally, based on the concepts studied, we found insufficiencies in the methodologies that combine the main parameters to estimate the physiological workload among nursing professionals.