Interrogation of Inhibitor of Nuclear Factor κB α/Nuclear Factor κB (IκBα/NF-κB) Negative Feedback Loop Dynamics

Abstract

Background: Understanding the biological significance of feedback loops requires interrogation at multiple scales. Results: A nuclear factor B (NF-B) negative feedback reporter revealed stimulus-specific dynamics in cells and animals in vivo. Conclusion: Circulating tumor necrosis factor ␣ (TNF␣) doses are perceived by the liver as pulses. Significance: Bioluminescent imaging of live single cells and cell populations revealed reproducible behaviors that informed interpretation of in vivo data.

Curcumol induces HSC-T6 cell death through suppression of Bcl-2: Involvement of PI3K and NF-κB pathways

“…Degradation of IjBa enhance nuclear NF-jB DNA binding activity and transcriptional activation of its target genes, including Bcl-2 family members (Moss et al, 2012). NF-jB activity is increased in cultured activated HSCs but it is not required for the process of activation (Lang et al, 2000).…”

Preconditioning with Intravenous Colitic Cell-Free DNA Prevents DSS-Colitis by Altering TLR9-Associated Gene Expression Profile

“…Nevertheless, ablation of enterocyte-specific NF-jB signaling provokes massive intestinal inflammation [68]. The dynamics of IKB degradation and subsequent NF-jB transcriptional activity in response to diverse ways of stimulation may partly be involved in this phenomenon [69].…”

Quantitative imaging of mammalian transcriptional dynamics: from single cells to whole embryos

“…Among the variety of regulatory factors are both the frequency and strength of the stimulation signal [101–103] as well as mechanical features such as cell shape and microenvironment [104]. Importantly, changing the translocation dynamics could impact the expression profiles of NF-κB target genes in both cultured cells as well as live animals [101, 102, 105], pointing to functional roles of such oscillatory nuclear translocation.…”