Interrelationship of certain vitamins of the B group in aneurin, riboflavin and biotin deficiencies

Abstract

Nitrogen estimations carried out on the carefully separated enamel of sound premolar teeth give a mean value of 0071 + 0-015 % for the age group up to 16 yearsandO0083 + 0021 %for older age groups. These values are lower than those previously reported.

Biochemistry of Pork Muscle Structure. II. Preliminary Observations of Biopsy Samples versus Ultimate Muscle Structure a

“…Although this is a reported method for the determination of lactic dehydrogenase activity (Bhagvat and Devi, 1949;Kuznetsova et al, 1953), it is known to be very sensitive to the oxygen content of the tissue, DPN-DPNH ratio, pH, denaturation, and other factors. It has, therefore, not been strictly discussed as dehydrogenase activity but rather as methylene-blue reduction, which would be influenced by and encompass the various factors.…”

“…Final volumes were adjusted to contain 100 mg of wet tissue per ml of solution. The lactic dehydrogenase activity of the tissue suspensions was estimated by the modified method of Thunberg (Bhagvat and Devi, 1949). The results are expressed as pg of methylene blue reduced by 100 mg protein in the presence of lactate substrate during 1 hr.…”

“…The results are expressed as pg of methylene blue reduced by 100 mg protein in the presence of lactate substrate during 1 hr. The activity of pyruvic oxidase was determined by the ability of the tissue to oxidize pyruvic acid ill vitro at 37°C (Bhagvat and Devi, 1949). Two ml of the tissue suspension were incubated 1 hr with 100 mq of pyruvic acid at 37°C.…”