Interrelationship between the Microbial Communities of the Root Canals and Periodontal Pockets in Combined Endodontic-Periodontal Diseases

Abstract

Periodontal and Endodontic diseases are biofilm-related diseases. The presence of microorganisms in root canals (RCs) and the complex microbiota of periodontal pockets (PPs) contribute to the development of endodontic-periodontal diseases. This study performed a systemic analysis using state-of-the-art sequence data to assess the microbial composition of infected RCs and PPs to further assess the microbiota and verify the possibility of cross-infection between these sites. The microbiomes of these combined diseases were examined with a focus on the V3-V4 hypervariable region of the 16S rRNA gene. The number of species in PP was higher than in RC, and there was a predominance of obligate anaerobes and gram-negative bacteria. In the RCs, the genera Enterococcus, Parvimonas, Stomatobaculum predominated, in contrast, the PPs revealed a predominance of Enterococcus, Parvimonas, Stomatobaculum, Peptostreptococcus and Mogibacterium. The RC and PP microbiome was not similar with regards to the sharing of OTUs for phyla and genera (8 and 67, respectively). The evaluation of molecular markers revealed a large number of markers for resistance to antibiotics of the carbapenem and beta-lactam type (broad spectrum). Another relevant finding of this study was the markers related to systemic diseases related to cardiac muscle and rheumatology, among others. In conclusion, the RC microbiota was less complex and diverse than PP. Interactions between microbial communities were present. The shared genus can signal communication between the endodontic and periodontal microbiomes.

Comparison of the Treatment Efficacy of Endo−Perio Lesions Using a Standard Treatment Protocol and Extended by Using a Diode Laser (940 nm)

“…The bacteria that are found in both of these environments are very similar. This similarity between bacteria is related to specific conditions, and the occurrence in anaerobic environments [9]. Some studies have shown that most bacteria are located in the outer 300 µm of dentinal tubules.…”

Next‐generation antibacterial nanopolymers for treating oral chronic inflammatory diseases of bacterial origin

“…In terms of the microbiotas associated with either periapical and periodontal diseases, although there are specific pathogens identified in each of these conditions and there is a high inter‐individual variability, there are also similarities, since the predominant microbiota of both diseases is mainly composed of obligate and facultative anaerobic bacteria and they share relative abundances of common microbial communities (Gomes et al., 2015; Kobayashi et al., 1990; Lopes et al., 2021; Pereira et al., 2011; Siqueira & Rôças, 2009, 2022). In fact, more than 68 common taxa have been identified in both root canal and periodontal pocket biofilms (Lopes et al., 2021) and pathobionts such as Fusobacterium nucleatum , Dialister species, Porphyromonas endodontalis , Porphyromonas gingivalis , Prevotella species, Tannerella forsythia and Treponema species have also been identified in both (Siqueira & Rôças, 2022).…”