Interrelations between Uptake of [14C]Orotic Acid and Labeling of UTP and RNA in Rat and Mouse Liver after Partial Hepatectomy

Yngner, Torsten; Carlberg, Elisabet; Lewan, Lillemor; Engelbrecht, Claes

doi:10.1515/bchm2.1979.360.2.1069

Cited by 9 publications

(7 citation statements)

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“…In regenerating rat liver, however, an increased transcription has been shown with this method (Bresnick, 1971;Bucher & Malt, 1971;Lewan et al, 1977). The different pictures revealed by mouse liver and rat liver when labelled orotic acid is used as precursor may depend on the fact that rat liver is extracting orotic acid from blood far more effectively than does mouse liver (Yngner et al, 1979a;Durschlag & Robinson, 1980). The 'de novo' pathway is the dominating route for pyrimidine-nucleotide synthesis in the rat (Yu & Feigelson, 1970;Hogans et al, 1971;Witschi, 1972), whereas in mouse liver both the 'de novo' and the salvage pathways for synthesis of nucleotides and RNA are well utilized .…”

Section: Resultsmentioning

confidence: 99%

“…The increased synthesis of rRNA in regenerating mouse liver cannot be demonstrated by incorporation of labelled orotic acid (Yngner et al, 1979a(Yngner et al, , 1980. In regenerating rat liver, however, an increased transcription has been shown with this method (Bresnick, 1971;Bucher & Malt, 1971;Lewan et al, 1977).…”

Section: Resultsmentioning

confidence: 99%

“…In contrast with regenerating rat liver, mouse liver reveals no enhanced uptake of orotic acid or incorporation of this precursor into either UTP or RNA at 6 and 24h after partial hepatectomy (Yngner et al, 1979a(Yngner et al, , 1980, nor is there an increased incorporation of labelled uridine into UTP or RNA of regenerating mouse liver, despite an increased uptake of the precursor into the total acid-soluble fraction (Yngner et al, 1979b).…”

mentioning

confidence: 83%

“…There is an enhanced uptake and incorporation of labelled orotic acid into rat liver RNA early after partial hepatectomy (Bresnick, 1971;Bucher & Malt, 1971). Since the specific radioactivity of RNA is increased more than that of UTP, there are strong indications of an enhanced RNA synthesis (Bucher & Swaffield, 1969;Yngner et al, 1979a). This interpretation is supported by the fact that the activities of RNA polymerases I, II and III are elevated at 18 h after partial hepatectomy (Organtini et al, 1975;Yu, 1975b;Duceman & Jacob, 1980).…”

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confidence: 93%

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RNA synthesis in regenerating mouse liver evaluated by incorporation of [methyl-14C]methionine and by determination of RNA polymerase activity

Ljungquist¹,

Yngner²,

Lewan³

et al. 1984

Biochemical Journal

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The synthesis of RNA during mouse liver regeneration was studied by two different methods at 24 and 48 h after partial hepatectomy. Total chromatin-bound RNA polymerase activity showed an increase of 32% at 24 h after partial hepatectomy. At 48 h a slight increase in total activity was also observed in regenerating liver, but the difference was not significant. The increase in total RNA polymerase activity was due to a rise in RNA polymerase I plus III activity. This enzyme activity was increased at both 24 and 48 h. The increase was 57% at 24 h and 51% at 48 h. When [methyl-14C]methionine was used for labelling of methyl groups in rRNA, there was an increased specific radioactivity of this class of RNA at both 24 h and 48 h. The increases were 263 and 103% at 24 and 48 h respectively. Thus both methods revealed an increased synthesis of rRNA during mouse liver regeneration. The results are discussed in relation to previous results from this laboratory [Yngner, Carlberg, Lewan & Engelbrecht (1979) Hoppe-Seyler's Z. Physiol. Chem. 360, 1069-1074; Yngner, Engelbrecht, Lewan & Annerfeldt (1979) Biochem. J. 178, 1-8; Yngner, Bengtsson, Carlberg, Engelbrecht & Wieslander (1980) Exp. Cell. Biol. 48, 393-403], which have shown that the incorporation of orotic acid or uridine into RNA is not increased in mouse liver regenerating after partial hepatectomy.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 83%

mentioning

confidence: 93%

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RNA synthesis in regenerating mouse liver evaluated by incorporation of [methyl-14C]methionine and by determination of RNA polymerase activity

Ljungquist¹,

Yngner²,

Lewan³

et al. 1984

Biochemical Journal

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“…An almost 2-fold increased [14C]-orotic acid incorporation in total acid-soluble fraction and uridine triphosphate (UTP) was observed in regenerating rat liver 24 h after operation (34). The RNA labelling increased more than that of UTP, thus indicating an increased RNA biosynthesis (8).…”

Section: Introductionmentioning

confidence: 98%

Possible Significance of Changes in the Energy Metabolism for the Release of Liver Lactate Dehydrogenase and for the Uptake and Incorporation of [14C]-Orotic Acid into Liver Ribonucleic Acid after Partial Hepatectomy

Yngner¹,

Bengtsson²,

Carlberg³

et al. 1980

Pathobiology

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The mouse liver revealed no increased incorporation of [¹⁴C]-orotic acid into either the total acid-soluble fraction, the uridine triphosphate or the RNA at 6 and 24 h after partial hepatectomy. In regenerating mouse and rat liver, the concentration of adenosine triphosphate was decreased 15–20% at 6 h, but was in the same range as that of the controls at 24 h. The adenosine monophosphate concentration of mouse liver increased 4-fold and 2-fold at these times after partial hepatectomy, respectively. The results indicate no direct relationship between the energy metabolism and the uptake and incorporation of orotic acid into RNA of regenerating liver. The activity of mouse plasma lactate dehydrogenase 5 (LDH 5) was increased 12-fold at 6 h and 5-fold at 24 h after partial hepatectomy. In rat, the LDH 5 activity was increased 2-fold at 6 h but was not different from that of the controls at 24 h. An increased leakage of LDH 5, possibly related to the decreased energy content of the liver, was thus revealed by the partially hepatectomized mice.

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Effects of partial hepatectomy on rna polymerase activities in mouse liver

Ljungquist

Åström

1984

International Journal of Biochemistry

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Interrelations between Uptake of [¹⁴C]Orotic Acid and Labeling of UTP and RNA in Rat and Mouse Liver after Partial Hepatectomy

Cited by 9 publications

References 1 publication

RNA synthesis in regenerating mouse liver evaluated by incorporation of [methyl-14C]methionine and by determination of RNA polymerase activity

RNA synthesis in regenerating mouse liver evaluated by incorporation of [methyl-14C]methionine and by determination of RNA polymerase activity

Possible Significance of Changes in the Energy Metabolism for the Release of Liver Lactate Dehydrogenase and for the Uptake and Incorporation of [<sup>14</sup>C]-Orotic Acid into Liver Ribonucleic Acid after Partial Hepatectomy

Effects of partial hepatectomy on rna polymerase activities in mouse liver

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