Interregional phase-amplitude coupling between theta rhythm in the nucleus tractus solitarius and high-frequency oscillations in the hippocampus during REM sleep in rats

Abstract

Cross-frequency coupling (CFC) between theta and high frequency oscillations (HFOs) is predominant during active wakefulness, REM sleep and behavioral and learning tasks in rodent hippocampus. Evidence suggests that these state-dependent CFCs are linked to spatial navigation and memory consolidation processes. CFC studies currently include only the cortical and subcortical structures. To our knowledge, the study of nucleus tractus solitarius (NTS)-cortical structure CFC is still lacking. Here we investigate CFC in simultaneous local field potential recordings from hippocampal CA1 and the NTS during behavioral states in freely moving rats. We found a significant increase in theta (6-8 Hz)-HFO (120-160 Hz) coupling both within the hippocampus and between NTS theta and hippocampal HFOs during REM sleep. Also, the hippocampal HFOs were modulated by different but consistent phases of hippocampal and NTS theta oscillations. These findings support the idea that phase-amplitude coupling is both state- and frequency-specific and CFC analysis may serve as a tool to help understand the selective functions of neuronal network interactions in state-dependent information processing. Importantly, the increased NTS theta-hippocampal HFO coupling during REM sleep may represent the functional connectivity between these two structures which reflects the function of the hippocampus in visceral learning with the sensory information provided by the NTS. This gives a possible insight into an association between the sensory activity and REM-sleep dependent memory consolidation.

Phase–Amplitude Coupling between Theta Rhythm and High-Frequency Oscillations in the Hippocampus of Pigeons during Navigation

“…Different hippocampal neurons were found to be locked to different theta phases in rodents and humans [49,50]. At the same time, there were significant phase differences when interregional CFC occured [51]. Subsequently, we found that after changing the navigation path and goal, this PAC phenomenon remained unchanged, implying that PAC appears to be specifically related to the progression of navigation.…”